New Forests

, Volume 44, Issue 2, pp 281–295 | Cite as

Growth response and nitrogen use physiology of Fraser fir (Abies fraseri), red pine (Pinus resinosa), and hybrid poplar under amino acid nutrition

  • Alexa R. Wilson
  • Pascal Nzokou
  • Deniz Güney
  • Şemsettin Kulaç


Plants can assimilate amino acids from soils. This has been demonstrated in controlled environments and soils of various forest ecosystems. However, the role of root-absorbed amino acids in plant nitrogen nutrition is still poorly understood. We investigated the agroecological performance and nutrient use physiology of two conifers (Abies fraseri and Pinus resinosa) and one hardwood species (hybrid poplar) under amino acid fertilization. Arginine fertilizer (arGrow® Complete) was applied at varying rates (0, 56, 112, 224, and 336 kg N/ha) and compared to an inorganic control treatment (ammonium sulfate 112 kg N/ha). Parameters monitored included tree growth response, foliar nitrogen concentration, and inorganic nitrogen leaching below the rootzone. Results obtained indicate a significant growth and foliar nitrogen response to amino acid treatments, with increasing amino acid application leading to greater growth and foliar nitrogen. However, rates two to three times higher than that of the inorganic control were necessary to provide similar growth and foliar nitrogen responses. These observations were suggested to be due to competition with soil microbes for organic nitrogen, growth inhibition due to the presence of large concentrations of amino acids, or adsorption to cation exchange sites. Amino acid applications did not increase the leaching of inorganic nitrogen due either to the binding of positively charged arginine cations to exchange sites or rapid mineralization followed by plant assimilation. Mineral nitrogen collected in leachate samples increased with the application rate suggesting at least some mineralization in high amino acid application rates. We conclude that growth response and nitrogen use physiology of these species when treated with arginine are largely controlled by soil processes including microbial competition and adsorption. Further studies are being conducted to confirm these hypotheses.


Organic nitrogen Arginine Nitrogen leaching Tree nutrition Short rotation cropping systems 



Special thank to SweTree Technologies for providing the Amino Acid Fertilizer used in this study and for technical support. This study was financially supported by the MSU AgBioResearch Station and the Michigan Seedlings Growers Association.


  1. Andresen LC, Michelsen A, Jonasson S, Beier C, Ambus P (2009) Glycine uptake in heath plants and soil microbes responds to elevated temperature, CO2 and drought. Acta Oecol 35:786–796CrossRefGoogle Scholar
  2. Bonner CA, Jensen RA (1997) Recognition of specific patterns of amino acid inhibition of growth in higher plants, uncomplicated by glutamine-reversible ‘general amino acid inhibition’. Plant Sci 130:133–143CrossRefGoogle Scholar
  3. Dannenmann M, Simon J, Gasche R, Holst J, Naumann PS, Kogel-Knabner I, Knicker H, Mayer H, Schloter M, Pena R, Polle A, Rennenberg H, Papen H (2009) Tree girdling provides insight on the role of labile carbon in nitrogen partitioning between soil microorganisms and adult European beech. Soil Biol Biochem 41:1622–1631CrossRefGoogle Scholar
  4. Dickmann DI (2006) Silviculture and biology of short-rotation woody crops in temperate regions: then and now. Biomass Bioenergy 30:696–705CrossRefGoogle Scholar
  5. Gonod LV, Jones DL, Chenu C (2006) Sorption regulates the fate of amino acids lysine and leucine in soil aggregates. Eur J Soil Sci 57:320–329CrossRefGoogle Scholar
  6. Griffin KL, Winner WE, Strain BR (1995) Growth and dry matter portioning in Loblolly and Ponderosa pine seedlings in response to carbon and nitrogen availability. New Phytol 129:547–556CrossRefGoogle Scholar
  7. Harrison KA, Bol R, Bardgett RD (2008) Do plant species with different growth strategies vary in their ability to compete with soil microbes for chemical forms of nitrogen? Soil Biol Chem 40:228–237CrossRefGoogle Scholar
  8. Hawkins BJ, Robbins S (2010) pH affects ammonium, nitrate and proton fluxes in the apical region of conifer and soybean roots. Physiol Plant 138:238–247PubMedCrossRefGoogle Scholar
  9. Jones DL (1999) Amino acid biodegradation and its potential effects on organic nitrogen capture by plants. Soil Biol Biochem 31:613–622CrossRefGoogle Scholar
  10. Jones DL, Shannon D, Junvee-Fortune T, Farrar JF (2005) Plant capture of free amino acids is maximized under high soil amino acid concentrations. Soil Biol Biochem 37:179–181CrossRefGoogle Scholar
  11. Kielland K (1994) Amino acid absorption by arctic plants: implications for plant nutrition and nitrogen cycling. Ecology 75:2373–2383CrossRefGoogle Scholar
  12. Kielland K (1995) Patterns of free amino acids in arctic tundra soils. Biogeochemistry 31:85–98CrossRefGoogle Scholar
  13. Kielland K, McFarland JW, Ruess RW, Olson K (2007) Rapid cycling of organic nitrogen in taiga forest ecosystems. Ecosystems 10:360–368CrossRefGoogle Scholar
  14. Lipson D, Näsholm T (2001) The unexpected versatility of plants: organic nitrogen use and availability in terrestrial ecosystems. Oecologica 128:305–316CrossRefGoogle Scholar
  15. Liu X, Ko K, Kim S, Lee K (2008) Effect of amino acid fertilization on nitrate assimilation of leafy radish and soil chemical properties in high nitrate soil. Commun Soil Sci Plant Anal 39:269–281CrossRefGoogle Scholar
  16. Lloyd JE, Herms DA, Rose MA, Van Wagoner J (2006) Fertilization rate and irrigation scheduling in the nursery influence growth, insect performance, and stress tolerance of ‘Sutyzan’ Crabapple in the landscape. HortScience 41:442–445Google Scholar
  17. Näsholm T, Ekblad A, Nordin A, Giesler R, Hogberg M, Hogberg P (1998) Boreal forest plants take up organic nitrogen. Nature 392:914–916CrossRefGoogle Scholar
  18. Näsholm T, Huss-Danell K, Hogberg P (2000) Uptake of organic nitrogen in the field by four agriculturally important plant species. Ecology 81:1155–1161Google Scholar
  19. Näsholm T, Kielland K, Ganeteg U (2009) Uptake of organic nitrogen by plants. New Phytol 182:31–48PubMedCrossRefGoogle Scholar
  20. Nikiema P, Nzokou P, Rothstein D (2011) Effects of groundcover management on soil properties, tree physiology, foliar chemistry and growth in a newly established Fraser fir (Abies fraseri [Pursh] Poir) plantation in Michigan, United States of America. New Forests. doi: 10.1007/s11056-011-9274-8 Google Scholar
  21. Öhlund J, Näsholm T (2001) Growth of conifer seedlings on organic and inorganic nitrogen sources. Tree Physiol 21:1319–1326PubMedCrossRefGoogle Scholar
  22. Öhlund J, Näsholm T (2002) Low nitrogen losses with a new source of nitrogen for cultivation of conifer seedlings. Environ Sci Technol 36:4854–4859PubMedCrossRefGoogle Scholar
  23. Persson J, Näsholm T (2001) Amino acid uptake: a widespread ability among boreal forest plants. Ecol Lett 4:434–438CrossRefGoogle Scholar
  24. Persson J, Näsholm T (2002) Regulation of amino acid uptake in conifers by exogenous and endogenous nitrogen. Planta 215:639–644PubMedCrossRefGoogle Scholar
  25. Raab TK, Lipson DA, Monson RK (1996) Non-mycorrhizal uptake of amino acids by roots of the alpine sedge Kobresia myosuroides: implications for the alpine nitrogen cycle. Oecologia 108:488–494CrossRefGoogle Scholar
  26. Reeve JR, Smith JL, Carpenter-Boggs L, Reganold JP (2008) Soil-based cycling and differential uptake of amino acids by three species of strawberry (Frageria spp.) plants. Soil Biol Biochem 40:2457–2552CrossRefGoogle Scholar
  27. Rothstein DE, Cregg BM (2005) Effects of nitrogen form on nutrient uptake and physiology of Fraser fir (Abies fraseri). For Ecol Manage 219:69–80CrossRefGoogle Scholar
  28. Sauheitl L, Glaser B, Weigelt A (2009) Uptake of intact amino acids by plants depends on soil amino acid concentrations. Environ Exp Bot 66:145–152CrossRefGoogle Scholar
  29. Schobert C, Kockenberger W, Komor E (1988) Uptake of amino acids by plants from soil: a comparative study with castor bean seedlings grown under natural and axenic soil conditions. Plant Soil 109:181–188CrossRefGoogle Scholar
  30. Smith SE, Read DJ (2008) Mycorrhyzal symbiosis, 3rd edn. Academic Press, New YorkGoogle Scholar
  31. Timmer VR (1991) Interpretation of seedling analysis and visual symptoms. In: van den Driessche R (ed) Mineral nutrition of conifer seedlings. CRC Press, Boca Raton, pp 113–134Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Alexa R. Wilson
    • 1
  • Pascal Nzokou
    • 1
  • Deniz Güney
    • 2
  • Şemsettin Kulaç
    • 3
  1. 1.Department of ForestryMichigan State UniversityEast LansingUSA
  2. 2.Department of ForestryKaradeniz Technical UniversityTrabzonTurkey
  3. 3.Faculty of ForestryDüzce UniversityDüzceTurkey

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