Advertisement

New Forests

, Volume 39, Issue 3, pp 261–273 | Cite as

Mating patterns in an indoor miniature Cryptomeria japonica seed orchard as revealed by microsatellite markers

  • Yoshinari Moriguchi
  • Yoko Yamazaki
  • Hideaki Taira
  • Yoshihiko Tsumura
Article

Abstract

To evaluate the practical application of an indoor seed orchard, we compared the seed production, final germination rates and mating patterns of potted Cryptomeria japonica D. Don clones in two miniature seed orchards: one in a glasshouse and the other outdoors. There was no statistically significant difference in total seed production between the orchards (P = 0.275, ANOVA). However, the final germination rate of seeds produced in the indoor orchard was significantly lower than that of seeds produced in the outdoor orchard (P < 0.01, Wilcoxon test). The average self-fertilization rate was higher in the indoor orchard (27.2%) than in the outdoor orchard (5.6%), and this parameter was strongly associated with the reduced final germination rates of the seeds harvested in the glasshouse. Pollen contamination was considerably lower in the indoor seed orchard (48.9% in the outdoor seed orchard and 4.4% in the indoor seed orchard). χ2 tests indicated that the paternal contributions of each constituent clone differed significantly in both orchards (P < 0.001 in both cases). The strong variations in paternal contributions among indoor orchard clones was significantly correlated with their respective pollen production capacities (Spearman ρ = 0.693, P < 0.001). To reduce the high self-fertilization rates, we recommend the use of orchard clones with similar pollen production capacity in indoor seed orchards.

Keywords

Male reproductive success Pollen contamination Molecular marker SSR Conifer 

Notes

Acknowledgments

We thank M. Saito for his helpful advice. This work was partly supported by Research Fellowships from the Japan Society for the Promotion of Science for Young Scientists and Grant-in-Aid (Development of Technologies for Control of Pollen Production by Genetic Engineering) from the Forest Agency of Japan and Program for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry.

References

  1. Adams WT, Hipkins VD, Burczyk J, Randall WK (1997) Pollen contamination trends in a maturing Douglas-fir seed orchard. Can J For Res 27:131–134CrossRefGoogle Scholar
  2. Aho ML, Pulkkinen P (1993) Evaluation of the frost hardiness of Scots pine seed orchard crops using early freezing tests. Rep Found For Tree Breed 7:1–12Google Scholar
  3. Apsit VJ, Nakamura RR, Wheeler NC (1989) Differential male reproductive success in Douglas fir. Theor Appl Genet 77:681–684CrossRefGoogle Scholar
  4. Botstein D, White R, Skolnick M, Davis RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32:314–331PubMedGoogle Scholar
  5. Burczyk J, Part D (1997) Male reproductive success in Psedotsuga menziesii (Mirb.) Franco: the effects of spatial structure and flowering characteristics. Heredity 79:638–647CrossRefGoogle Scholar
  6. Doerksen TK, Herbinger CM (2008) Male reproductive success and pedigree error in red spruce open-pollinated and polycross mating systems. Can J For Res 38:1742–1749CrossRefGoogle Scholar
  7. Durham OC (1946) The volumetric incidence of atmospheric allergens IV. A proposed standard method of gravity sampling, counting and volumetric interpolation of results. J Allergy 17:79–86CrossRefGoogle Scholar
  8. Erickson VJ, Adams WT (1989) Mating success in a coastal Douglas-fir seed orchard as affected by distance and floral phenology. Can J For Res 19:1248–1255Google Scholar
  9. Friedman ST, Adams WT (1985) Estimation of gene flow into two seed orchards of loblolly pine (Pinus taeda L.). Theor Appl Genet 69:609–615CrossRefGoogle Scholar
  10. Furukoshi T (1978) Studies on pollen control in a seed orchard of Sugi, Cryptomeria japonica D. Don. Bull For For Prod Res Inst 300:41–120 (in Japanese)Google Scholar
  11. Goto S, Miyahara F, Ide Y (2002a) Contribution of pollen parents to the nematode-resistance in seedlings of Japanese black pine. J Jpn For Soc 84:45–49 (Japanese with English summary)Google Scholar
  12. Goto S, Miyahara F, Ide Y (2002b) Monitoring male reproductive success in a Japanese black pine clonal seed orchard with RAPD markers. Can J For Res 32:983–988CrossRefGoogle Scholar
  13. Goto S, Takahashi M, Matsumoto A, Ieiri R, Tsumura Y (2008) Genetic relationships and origin of commercial clones of Nangouhi, a vegetatively propagated cultivar of hinoki cypress (Chamaecyparis obtusa). Breed Sci 58:411–418CrossRefGoogle Scholar
  14. Itoo S (1984) Techniques of seed production in seed orchard of Cryptomeria japonica D. Don (I)—flowering traits and seed production of constituent clones. J Niigata For Prod Res Cent 26:1–22 (in Japanese)Google Scholar
  15. Itoo S (1985) Techniques of seed production in seed orchard of Cryptomeria japonica D. Don (II)—the seed production in the miniature clonal seed orchard. J Niigata For Prod Res Cent 27:1–13 (in Japanese)Google Scholar
  16. Itoo S, Katsuta M (1986) Seed productivity in the miniature clonal seed orchard of Cryptomeria japonica D. Don. J Jpn For Soc 68:284–288Google Scholar
  17. Jones ME, Shepherd M, Henry R, Delves A (2008) Pollen flow in Eucalyptus grandis determined by paternity analysis using microsatellite markers. Tree Genet Genomes 4:37–47CrossRefGoogle Scholar
  18. Longman KA, Dick JM (1981) Can seed-orchards be miniaturized? In: Proceedings of symposium on flowering physiology XVII IUFRO world congress, Kyoto, Japan, pp 98–102Google Scholar
  19. Moriguchi Y, Iwata H, Ihara T, Yoshimura K, Taira H, Tsumura Y (2003) Development and characterization of microsatellite markers for Cryptomeria japonica D. Don. Theor Appl Genet 106:751–758PubMedGoogle Scholar
  20. Moriguchi Y, Tani N, Taira H, Tsumura Y (2004) Variation of paternal contribution in a seed orchard of Cryptomeria japonica D. Don determined using microsatellite markers. Can J For Res 34:1683–1690CrossRefGoogle Scholar
  21. Moriguchi Y, Tani N, Itoo S, Kanehira F, Tanaka K, Yomogida H, Taira H, Tsumura Y (2005a) Gene flow and mating system in five Cryptomeria japonica D. Don seed orchards as revealed by analysis of microsatellite markers. Tree Genet Genomes 1:174–183CrossRefGoogle Scholar
  22. Moriguchi Y, Goto S, Takahashi M (2005b) Genetic management of seed orchards based on information revealed by molecular markers (in Japanese with English summary). J Jpn For Res 87:161–169Google Scholar
  23. Moriguchi Y, Tsuchiya S, Iwata H, Itoo S, Tani N, Taira H, Tsumura Y (2007) Factors influencing male reproductive success in a Cryptomeria japonica seed orchard revealed by microsatellite marker analysis. Silvae Genet 56:207–214Google Scholar
  24. Moriguchi Y, Ishiduka D, Kaneko T, Itoo S, Taira H, Tsumura Y (2009) The contribution of pollen germination rates to uneven paternity among polycrosses of Cryptomeria japonica. Silvae Genet 58:139–144Google Scholar
  25. Murray M, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321–4325CrossRefPubMedGoogle Scholar
  26. Nagasaka K, Tabuchi K (1975) Difference among plus tree clones for flowering ability in Cryptomeria japonica D. Don. Ann Bull Kanto For Tree Breed Inst 11:189–213 (in Japanese, with English summary)Google Scholar
  27. Nakamura RR, Wheeler NC (1992) Pollen competition and paternal success in Douglas-fir. Evolution 46:846–851CrossRefGoogle Scholar
  28. Ng KKS, Lee SL, Ueno S (2009) Impact of selective logging on genetic diversity of two tropical tree species with contrasting breeding systems using direct comparison and simulation methods. For Ecol Manag 257:107–116CrossRefGoogle Scholar
  29. Ohba K (1993) Clonal forestry with sugi (Cryptomeria japonica). In: Ahuja MR, Libby WJ (eds) Clonal forestry II: conservation and application. Springer, Berlin, pp 66–90Google Scholar
  30. Okawa H, Sawagashira N (1998) Comparison of seed production for each clone selected from Aomori prefecture in miniature seed orchard of Cryptomeria japonica D. Don. J Aomori For Res Cent: 8-17 (in Japanese)Google Scholar
  31. Pakkanen A, Nikkanen T, Pulkkinen P (2000) Annual variation in pollen contamination and outcrossing in a Picea abies seed orchard. Scand J For Res 15:399–404CrossRefGoogle Scholar
  32. Ritland K, El-Kassaby YA (1985) The nature of inbreeding in a seed orchard of Douglas fir as shown by an efficient multilocus model. Theor Appl Genet 71:375–384CrossRefGoogle Scholar
  33. Rudin D, Muona O, Yazdani R (1986) Comparison of the mating system of Pinus sylvestris in natural stands and seed orchards. Hereditas 104:15–19CrossRefGoogle Scholar
  34. Saito M (2009) Effectiveness of an indoor miniature seed orchard arranged in lines for Cryptomeria japonica retaining male-sterile genes. J Jpn For Soc 91:168–172 (in Japanese with English summary)CrossRefGoogle Scholar
  35. Saito M, Taira H (2006) Characteristics and effectiveness of glasshouse miniature seed orchards in Cryptomeria japonica D. Don. J Jpn For Soc 88:187–191 (in Japanese with English summary)CrossRefGoogle Scholar
  36. Schoen DJ, Stewart SC (1986) Variation in male reproductive investment and male reproductive success in white spruce. Evolution 40:1109–1120CrossRefGoogle Scholar
  37. Shen HH, Rudin D, Lindgren D (1981) Study of the pollination pattern in a Scots pine seed orchard by means of isozyme analysis. Silvae Genet 30:7–15Google Scholar
  38. Stoehr MU, Newton CH (2002) Evaluation of mating dynamics in a lodgepole pine seed orchard using chloroplast DNA markers. Can J For Res 32:469–476CrossRefGoogle Scholar
  39. Stoehr MU, Orvar BL, Gawley JR, Webber JE, Newton CH (1998) Application of a chloroplast DNA marker in seed orchard management evaluations of Douglas-fir. Can J For Res 28:187–195CrossRefGoogle Scholar
  40. Tani N, Takahashi T, Ujino-Ihara T, Iwata H, Yoshimura K, Tsumura Y (2004) Development and characteristics of microsatellite markers for sugi (Cryptomeria japonica D. Don) from microsatellite enriched libraries. Ann For Sci 61:569–575CrossRefGoogle Scholar
  41. Tsumura Y, Yoshimura K, Tomaru N, Ohba K (1995) Molecular phylogeny of conifers using RFLP analysis of PCR-amplified specific chloroplast genes. Theor Appl Genet 91:1222–1236CrossRefGoogle Scholar
  42. Wang XR, Lindgren D, Szmidt AE, Yazdani R (1991) Pollen migration into a seed orchard of Pinus sylvestris L., and the methods of its estimation using allozyme markers. Scand J For Res 6:379–385CrossRefGoogle Scholar
  43. Weir BS (1996) Genetic data analysis II. Sinauer Associates, Sunderland, pp 209–211Google Scholar
  44. Wheeler NC, Jech KS (1992) The use of electrophoretic markers in seed orchard research. New Forests 6:311–328CrossRefGoogle Scholar
  45. Yazdani R, Lindgren D (1991) Variation of pollen contamination in a Scots pine seed orchard. Silvae Genet 40:243–246Google Scholar
  46. Yokoyama T (1977) The cause of self-sterility in Cryptomeria japonica D. Don. J Jpn For Soc 59:389–390 (in Japanese)Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Yoshinari Moriguchi
    • 1
  • Yoko Yamazaki
    • 2
  • Hideaki Taira
    • 2
  • Yoshihiko Tsumura
    • 1
  1. 1.Department of Forest GeneticsForestry and Forest Products Research InstituteTsukubaJapan
  2. 2.Graduate School of Science and TechnologyNiigata UniversityIgarashi 2-nochoJapan

Personalised recommendations