Cellular stages of root formation, root system quality and survival of Pinus elliottii var. elliottii × P. caribaea var. hondurensis cuttings in different temperature environments
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Time to first root in cuttings varies under different environmental conditions and understanding these differences is critical for optimizing propagation of commercial forestry species. Temperature environment (15, 25, 30 or 35 ± 2°C) had no effect on the cellular stages in root formation of the Slash × Caribbean Pine hybrid over 16 weeks as determined by histology. Initially callus cells formed in the cortex, then tracheids developed and formed primordia leading to external roots. However, speed of development followed a growth curve with the fastest development occurring at 25°C and slowest at 15°C with rooting percentages at week 12 of 80 and 0% respectively. Cutting survival was good in the three cooler temperature regimes (>80%) but reduced to 59% at 35°C. Root formation appeared to be dependant on the initiation of tracheids because all un-rooted cuttings had callus tissue but no tracheids, irrespective of temperature treatment and clone.
KeywordsPropagation Stem anatomy Root anatomy Callus Cuttings Cortex Abiotic Pinus
We wish to thank Forestry Plantations Queensland for supporting this work with both cash and in kind resources and Santi Krisantini for providing valuable assistance with the preparation of the manuscript.
- Aimers-Halliday J, Menzies MI, Faulds T et al (2003) Nursery systems to control maturation in Pinus radiata cuttings comparing hedging and serial propagation. N Z J Sci 33:135–155Google Scholar
- Bredmose N, Kristiansen K, Nielsen B (2004) Propagation temperature, PPFD, auxin treatment, cutting size and cutting position affect root formation axillary bud growth and shoot development in miniature rose (Rosa hybrida L.) plants and alter homogeneity. J Hortic Sci Biotechnol 79:458–465Google Scholar
- Geneve RL, Hackett WP, Swanson BT (1998) Adventitious root formation in de-bladed petiols from juvenile and mature phases in English Ivy. J Am Soc Hortic Sci 113:6310–6635Google Scholar
- Harding KJ, Copley TR (2000) Review of wood property variation in queensland grown trials of Pinus elliottii var elliottii × Pinus caribaea var hondurensis hybrids. In: Dungey HS, Dieters MJ, Nikles DG (eds) Symposium on hybrid breeding and genetics. QFRI/CRC-SPF, Noosa Lakes Resort, Queensland, AustraliaGoogle Scholar
- Horgan K, Skudder D, Holden G (1997) Clonal storage and rejuvenation. IUFRO 97 genetics of radiata pine, vol 203. FRI Bulletin, Rotorua, New Zealand, pp 273–280Google Scholar
- Howard BW, Nahlawi N (1965) Factors affecting the rooting of plum hardwood cuttings. J Hortic Sci 44:303–310Google Scholar
- Kristiansen K, Bredmose N, Nielsen B (2005) Influence of propagation temperature, photosynthetic photon flux density, auxin treatment and cutting position on root formation, axillary bud growth and shoot development in Schlumbergera ‘Russian Dancer’. J Hortic Sci Biotechnol 80:297–302Google Scholar
- Mitchell RG (2002) The effect of bottom heat on rooting Pinus patula and Pinus elliottii × Pinus caribaea stem cuttings in South Africa. Sth Afr For J 2:1–25Google Scholar
- Nikles DG (1996) The first 50 years of the evolution of forest tree improvement in Queensland. In: Dieters MJ, Matheson AC, Nikles DG, Harwood CE, Walker SM (eds) Tree improvement for sustainable tropical forestry. QFRI-IUFRO, Caloundra, Queensland, Australia, pp 51–64Google Scholar
- Nikles DG (2000) Experience with some Pinus hybrids in Queensland, Australia. In: Dungey HS, Dieters MJ, Nikles DG (eds) Hybrid breeding and genetics of forest trees. Proceedings of QFRI/CRC-SPF Symposium, Noosa, Queensland, AustraliaGoogle Scholar
- Ying CC, Bagley WT (1977) Variation in rooting capabilities of Populus deltoides. Silvae Genet 26:204–207Google Scholar