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New Forests

, Volume 31, Issue 3, pp 453–467 | Cite as

Effect of Scleroderma Spore Density and Age on Mycorrhiza Formation and Growth of Containerized Eucalyptus globulus and E. urophylla Seedlings

  • Ying Long Chen
  • Bernard Dell
  • Nicholas Malajczuk
Article

Abstract

Greenhouse trials with containerized Eucalyptus globulus and E. urophylla seedlings were made to determine effective inoculum spore densities (Scleroderma cepa from temperate Australia, and S. citrinum from sub-tropical China) and spore storage conditions (fresh spores or spores stored for 5 years at room temperature or at 4 °C; S. albidum, S. areolatum and S. cepa from Western Australia). Inoculation with 106 or 108 spores/seedling increased eucalypt growth by up to 46% in height and 42% in dry weight compared to non-inoculated seedlings at 12 weeks after inoculation. Mycorrhizal formation was poor at 102 spores/seeding. Spores stored at 4 °C for 5 years were as effective in forming mycorrhizas as freshly collected spores when a standard density of 106 spores/seedling was applied. It is recommended that spore densities ≥104 be used for inoculation of containerised eucalypts and that spores be stored at 4 °C until use.

Keywords

Ectomycorrhiza Eucalypt Nursery Scleroderma Spore density Spore storage 

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Copyright information

© Springer 2006

Authors and Affiliations

  • Ying Long Chen
    • 1
    • 2
  • Bernard Dell
    • 1
  • Nicholas Malajczuk
    • 1
  1. 1.School of Biological Sciences and BiotechnologyMurdoch UniversityPerthAustralia
  2. 2.Research Institute of Tropical ForestryChinese Academy of ForestryGuangzhouChina

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