A Method for Detecting Histaminergic Neurons in the Hypothalamus

Objective. To develop an immunohistochemical method for detecting histaminergic neurons in the rat hypothalamus. Materials and methods. Experiments were performed on hypothalamic specimens from 10 mongrel white male rats aged 45 and 90 days. Selective detection of histaminergic neurons on paraffin hypothalamic sections was by an immunohistochemical reaction for antibodies to monoamine oxidase type B. Results. A method for the selective detection of histaminergic neurons in the hypothalamus on paraffin sections using an immunohistochemical reaction for antibodies to monoamine oxidase type B is described. Conclusions. The method provided for detection of histaminergic neurons in paraffin sections of the hypothalamus.

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Correspondence to S. M. Zimatkin.

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Translated from Morfologiya, Vol. 156, No. 4, pp. 102–105, July–August, 2019.

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Zimatkin, S.M., Zaerko, A.V. A Method for Detecting Histaminergic Neurons in the Hypothalamus. Neurosci Behav Physi (2020). https://doi.org/10.1007/s11055-020-00949-4

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Keywords

  • histaminergic neurons
  • hypothalamus
  • rat brain
  • monoamine oxidase B