Studies of the Dynamics of Transmitter Contents in Neurons in the Sympathetic Ganglia in Cats in Early Ontogeny

Objectives. To determine the location and immunohistochemical characteristics of neurons in the superior cervical (SCG) and stellate (CG) ganglia of the sympathetic part of the peripheral nervous system in cats. Materials and methods. Studies were carried out on cats aged 1, 10, 20, and 30 days using retrograde axonal transport of fast blue (FB) given into the muscles of the neck, along with immunohistochemical methods. Results. FB-labeled neurons were detected in both of the sympathetic ganglia studied in kittens from birth. Most of these neurons were immunoreactive (IR) for tyrosine hydroxylase in all age groups. The proportion of labeled neurons simultaneously reacting to antibody to TH and neuropeptide Y in both ganglia increased in the first 10 days of life. The percentage content of calbindin-IR neurons in the SCG decreased from birth to day 30 of life while the number in the CG increased in the first 10 days and then decreased by day 30. Reactions for antibody to choline acetyltransferase, vasoactive intestinal polypeptide, and neuronal nitric oxide synthase were seen in fewer than 1% of labeled neurons in kittens of all age groups. Somatostatin-IR-labeled neurons were not seen in the SCG. Conclusions. The composition of neurons in the sympathetic ganglia completed ontogenetic rearrangement in kittens by day 20 of life.

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References

  1. 1.

    P. M. Maslyukov, “Connections between neurons in the stellate ganglion of cats with target organs during postnatal ontogeny,” Ros. Fiziol. Zh., 86, No. 6, 703–710 (2000).

    Google Scholar 

  2. 2.

    P. M. Maslyukov, A. A. Korobkin, V. V. Konovalov, et al., “Agerelated development of calbindin-immunopositive neurons in the sympathetic ganglia in rats,” Morfologiya, 141, No. 1, 77–80 (2012).

    Google Scholar 

  3. 3.

    P. M. Maslyukov, A. I. Emanuilov, and A. D. Nozdrachev, “Agerelated changes in the neurotransmitter component of neurons in the sympathetic ganglia,” Usp. Gerontol., 29, No. 3, 442–453 (2016).

    Google Scholar 

  4. 4.

    P. M. Maslyukov, A. D. Nozdrachev, and A. I. Emanuilov, “Agerelated characteristics of the expression of calcium-binding proteins in neurons in the ganglia of the autonomic nervous system,” Usp. Gerontol., 29, No. 2, 247–253 (2016).

    Google Scholar 

  5. 5.

    A. D. Nozdrachev and P. M. Maslyukov, “Neuropeptide Y and the autonomic nervous system,” Zh. Évolyuts. Biokhim. Fiziol., 47, No. 2, 105–112 (2011).

    CAS  Google Scholar 

  6. 6.

    C. R. Anderson, R. M. McAllen, and S. L. Edwards, “Nitric oxide synthase and chemical coding in cat sympathetic postganglionic neurons,” Neuroscience, 68, No. 1, 255–264 (1995), doi: https://doi.org/10.1016/0306-4522(95)00143-7.

    CAS  Article  PubMed  Google Scholar 

  7. 7.

    W. H. Chan, C. R. Anderson, and D. G. Gonsalvez, “From proliferation to target innervation: signaling molecules that direct sympathetic nervous system development,” Cell Tissue Res., 372, No. 2, 171–193 (2018), doi: https://doi.org/10.1007/s00441-017-2693-x.

    CAS  Article  PubMed  Google Scholar 

  8. 8.

    U. Ernsberger and H. Rohrer, “Sympathetic tales: subdivisons of the autonomic nervous system and the impact of developmental studies,” Neural Dev., 13, No. 1, 20 (2018), doi: https://doi.org/10.1186/s13064-018-0117-6.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  9. 9.

    I. Fliniaux, E. Germain, V. Farfariello, and N. Prevarskaya, “TRPs and Ca2+ in cell death and survival,” Cell Calcium, 69, 4–18 (2018), doi: https://doi.org/10.1016/j.ceca.2017.07.002.

    CAS  Article  PubMed  Google Scholar 

  10. 10.

    R. J. Gasperini, M. Pavez, A. C. Thompson, et al., “How does calcium interact with the cytoskeleton to regulate growth cone motility during axon pathfinding?” Mol. Cell. Neurosci., 84, 29–35 (2017), doi: https://doi.org/10.1016/j.mcn.2017.07.006.

    CAS  Article  PubMed  Google Scholar 

  11. 11.

    P. M. Masliukov, “Sympathetic neurons of the cat stellate ganglion in postnatal ontogenesis: morphometric analysis,” Auton. Neuroscientist, 89, No. 1–2, 48–53 (2001).

    CAS  Article  Google Scholar 

  12. 12.

    P. M. Masliukov, A. I. Emanuilov, K. Moiseev, et al., “Development of noncatecholaminergic sympathetic neurons in para- and prevertebral ganglia of cats,” Int. J. Dev. Neurosci., 40, 76–84 (2015), doi: https://doi.org/10.1016/j.ijdevneu.2014.12.004.

    CAS  Article  PubMed  Google Scholar 

  13. 13.

    P. M. Masliukov, V. V. Konovalov, A. I. Emanuilov, and A. D. Nozdrachev, “Development of neuropeptide Y-containing neurons in sympathetic ganglia of rats,” Neuropeptides, 46, No. 6, 345–352 (2012), doi: https://doi.org/10.1016/j.npep.2012.08.003.

    CAS  Article  PubMed  Google Scholar 

  14. 14.

    P. M. Maslyukov, M. B. Korzina, A. I. Emanuilov, and V. V. Shilkin, “Neurotransmitter composition of neurons in the cranial cervical and celiac sympathetic ganglia in postnatal ontogenesis,” Neurosci. Behav. Physiol., 40, No. 2, 143–147 (2010), doi: https://doi.org/10.1007/s11055-009-9247-y.

    CAS  Article  PubMed  Google Scholar 

  15. 15.

    R. Saraf, F. Mahmood, R. Amir, and R. Matyal, “Neuropeptide Y is an angiogenic factor in cardiovascular regeneration,” Eur. J. Pharmacol., 776, 64–70 (2016), doi: https://doi.org/10.1016/j.ejphar.2016.02.033.

    CAS  Article  PubMed  Google Scholar 

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Correspondence to A. I. Emanuilov.

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Translated from Morfologiya, Vol. 156, No. 5, pp. 44–48, September–October, 2019.

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Emanuilov, A.I. Studies of the Dynamics of Transmitter Contents in Neurons in the Sympathetic Ganglia in Cats in Early Ontogeny. Neurosci Behav Physi 50, 650–654 (2020). https://doi.org/10.1007/s11055-020-00948-5

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Keywords

  • autonomic nervous system
  • sympathetic ganglia
  • ontogeny