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Identification of Phosphoethanolamine and Phosphoserine in the Brain of the Pond Fish Perccottus Glehni (Eleotridae, Perciformes, Dyb. 1877)

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Significant modifications to the free amino acid pool and various other compounds in the bodies of poikilotherms in response to decreased temperature reflect significant alterations in their mechanisms of adaptation. The literature lacks data on the contributions of such free compounds to the low-temperature adaptation of the brains of poikilotherms. Our previous studies showed that acute cold shock induced the appearance of large quantities of two ninhydrin-positive compounds of unknown nature in the brain of the eurythermal pond fish Amur sleeper. The experiments reported here show that the brain accumulates these compounds by the beginning of the winter period. They were found to be the phospholipid metabolites phosphoethanolamine and phosphoserine. The winter phosphoethanolamine pool was 94 times greater than the summer level, while phosphoserine was present only in summer. It is suggested that accumulation of phosphoethanolamine and phosphoserine is associated with adaptive modifications of membrane phospholipids at low temperatures.

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References

  1. M. V. Karanova, “Changes in free amino acid contents in the tissue fluids of the freshwater mollusk Lymnaea stagnalis during seasonal adaptation to low positive temperatures,” Izv. Rus. Akad. Nauk. Ser. Biol., No. 6, 719–724 (2006).

  2. M. V. Karanova, “The free amino acid contents of blood and muscle in the Amur sleeper Perccottus glehni during preparation for and completion of hibernation,” Zh. Evolyuts. Biokhim. Fiziol., 45, No. 1, 59–67 (2009).

    CAS  Google Scholar 

  3. M. V. Karanova, “Effects of acute cold shock on free amino acid pools in the pond fish Amur sleeper Perccottus glehni (Eleotridae, Perciformes),” Izv. Rus. Akad. Nauk. Ser. Biol., No. 2, 1–9 (2011).

  4. M. V. Karanova and A. A. Andreev, “Free amino acids and reducing sugars in the amphipod Gammarus lacustris (Crustacea, Amphipoda) at the initial stage of preparation for the winter season,” Zh. Evolyuts. Biokhim. Fiziol., 46, No. 4, 279–283 (2010).

    CAS  Google Scholar 

  5. M. V. Karanova and E. N. Gakhova, “The molecular nature of the cryoresistance of the lake amphipod Gammarus lacustris,,” Biofizika, 47, No. 1, 116–124 (2002).

    CAS  PubMed  Google Scholar 

  6. M. V. Karanova and E. N. Gakhova, “Biochemical strategy for survival in the freshwater mollusk Lymnaea stagnalis at near-zero temperatures,” Zh. Evolyuts. Biokhim. Fiziol., 43, No. 3, 258–264 (2007).

    CAS  Google Scholar 

  7. R. A. Ahokas and G. Sorg, “Osmoregulation and muscle free amino acids in Fundulus diaphanous,” Comp. Biochem. Physiol., 56A, 101–105 (1977).

    Article  Google Scholar 

  8. K. A. Al-Badry and T. M. Hoda, “Hibernation hypothermia and metabolism in hedgehogs – changes in free amino acids and related compounds,” Comp. Biochem. Physiol., 72A, No. 3, 541–547 (1982).

    Article  CAS  Google Scholar 

  9. M. Bidri and P. Choay, “Taurine: a particular amino acid with multiple functions,” Ann. Pharm. Fr., 61, No. 6, 385–391 (2003).

    CAS  PubMed  Google Scholar 

  10. C. Buda, I. Dey, N. Baloght, et al., “Structural order of membranes and composition of phospholipids in fish brain cells during thermal acclimatization,” Proc. Natl. Acad. Sci. USA, 91, 8234–8238 (1994).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. A. Calabretti, F. Cateni, G. Procida, and L. G. Favretto, “Influence of environmental temperature on composition of lipids in edible flesh of rainbow trout (Oncorhynchus mykiss),” J. Science Food Agric., 83, No. 14, 1493–1498 (2003).

    Article  CAS  Google Scholar 

  12. M. C. J. Chang and B. I. Roots, “Influence of environmental temperature on the phospholipid composition of outer and inner mitochondrial membranes from goldfish (Carassius auratus L.) brain,” J. Therm. Biol., 13, No. 2, 61–66 (1988).

    Article  CAS  Google Scholar 

  13. T. A. Churchill and K. B. Storey, “Freezing survival of the garter snake Thamnophis sirtalis parietalis,” Can. J. Zool., 70, No. 1, 99–105 (1992).

    Article  Google Scholar 

  14. T. A. Churchill and K. B. Storey, “Metabolic responses to freezing and anoxia by the periwinkle Littorina littorea,” J. Therm. Biol., 21, No. 1, 57–63 (1996).

    Article  Google Scholar 

  15. F. Gibellini and T. Smith, “The Kennedy pathway – de novo synthesis of phosphatidylethanolamine and phosphatidylcholine,” Int. Union Biochem. Mol. Biol., 62, 414–428 (2010).

    CAS  Google Scholar 

  16. J. Gras, Y. Gudefi n, and F. Chagny, “Free amino-acids and ninhydrin-positive substances in fish. I. Muscle and skin of the rainbow trout (Salmo gairdnerii richardson),” Comp. Biochem. Physiol. B. Biochem. Mol. Biol., 60, No. 4, 369–372 (1978).

    Article  Google Scholar 

  17. R. Hanzal and A. Jegorov, “Changes in free amino acid composition in haemolymph of larvae of the wax moth, Galleria mellonella L. during cold acclimation,” Comp. Biochem. Physiol., 100A, 957–962 (1991).

    Article  CAS  Google Scholar 

  18. Y. Hirabayashi and S. Furuya, “Roles of L-serine and sphingolipid synthesis in brain development and neuronal survival,” Prog. Lipid Res., 47, No. 3, 188–203 (2008).

    Article  CAS  PubMed  Google Scholar 

  19. P. W. Hochachka and G. N. Somero, Biochemical Adaptation, Mechanism and Process in Physiological Evolution, Oxford University Press, Oxford (2002).

    Google Scholar 

  20. M. V. Karanova, “Influence of low temperature on the evolution of amino acid pools adaptive modifications in poikilothermic animals (review article),” Int. J. Biochem. Biophys., 1, No. 2, 33–40 (2013), DOI: 10.13189.ijbb.010202, www.hrpub.org.

    Google Scholar 

  21. H. Pasantes-Morales and A. Schousboe, “Role of taurine in osmoregulation in brain cells: Mechanisms and functional implications,” Amino Acids, 12, No. 3–4, 281–292 (1997).

    Article  CAS  Google Scholar 

  22. Z. Pavlovic and M. Bukovic, “Regulation of phosphatidylethanolamine homeostasis-the critical role of CTP:Phosphoethanolamine cytidylyltransferase (Pcyt2),” Int. J. Mol. Sci., 14, 2529–2550 (2013).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. R. Rosa, R. Calado, A. Andrade, et al., “Changes in amino acids and lipids during embryogenesis of European lobster, Homarus gammarus (Crustacea: Decapoda),” Comp. Biochem. Physiol., 149B, No. 2, 241–249 (2005).

    Article  Google Scholar 

  24. J. P. Slotte, “Biological functions of sphingomyelins,” Progr. Lipid Res., 52, No. 4, 424–437 (2013).

    Article  CAS  Google Scholar 

  25. D. H. Spachman, W. H. Stein, and S. Moore, “Automatic recording apparatus for use in the chromatography of amino acids,” Anal. Chem., 30, No. 7, 1190–1206 (1958).

    Article  Google Scholar 

  26. K. B. Storey and T. A. Churchill, “Metabolic responses to anoxia and freezing by the freeze-tolerant marine mussel Geukensia demissus,” J. Exp. Mar. Biol. Ecol., 188, No. 1, 99–114 (1995).

    Article  Google Scholar 

  27. K. B. Storey, J. M. Storey, S. P. J. Brooks, et al., “Hatchling turtles survive freezing during winter hibernation,” Proc. Natl. Acad. Sci. USA, 85, 8350–8354 (1988).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  28. C. Vines, “Phospholipase C,” Adv. Exp. Med. Biol., 740, 235–254 (2012).

    Article  CAS  PubMed  Google Scholar 

  29. J. Vance, “Phosphatidylserine and phosphatidylethanolamine in mammalian cells: Two metabolically-related aminophospholipids,” J. Lipid Res., 49, 1377–1387 (2008).

    Article  CAS  PubMed  Google Scholar 

  30. J. Vance and G. Tasseva, “Formation and function of phosphatidylserine and phosphatidylethanolamine in mammalian cells,” Biochim. Biophys. Acta, 1831, No. 3, 543–554 (2013).

    Article  CAS  PubMed  Google Scholar 

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Karanova, M.V. Identification of Phosphoethanolamine and Phosphoserine in the Brain of the Pond Fish Perccottus Glehni (Eleotridae, Perciformes, Dyb. 1877). Neurosci Behav Physi 46, 803–807 (2016). https://doi.org/10.1007/s11055-016-0314-x

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  • DOI: https://doi.org/10.1007/s11055-016-0314-x

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