Comparative Study of Catecholaminergic and Nitroxidergic Neurons in the Vasomotor Nuclei of the Caudal Part of the Brainstem in Rats
Immunohistochemical studies in 12 Wistar rats detected tyrosine hydroxylase (TH) and the neuronal form of nitric oxide synthase (nNOS) to address the distribution of catecholaminergic and nitroxidergic neurons, respectively, in the vasomotor nuclei of the medulla oblongata and pons. The highest frequency of TH expression was seen in neurons located in these nuclei and some reticular nuclei (the gigantocellular, paragigantocellular, and caudal nuclei of the pons), though the proportions of immunoreactive neurons in these structures were no greater than 8–14%. In other nuclei (the reticular parvocellular and oral nucleus of the pons, the spinal nucleus of the trigeminal nerve), this proportion ranged from 1 to 3%. Neurons of this type were consistently not detected in a large group of nuclei with confirmed vasomotor functions. Structures with many catecholaminergic neurons generally contained fewer nNOS-positive cells than nuclei with limited numbers of TH-positive neurons.
Keywordsmedulla oblongata pons vasomotor nuclei catecholaminergic and nitroxidergic neurons
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- 1.A. A. Afanas’ev, A. E. Kotsyuba, and V. M. Chertok, “Allego-MC, a system for the automated analysis of images of micro- and macrostructures,” Tikhookean. Med. Zh., No. 4, 65–68 (2002).Google Scholar
- 2.E. V. Babich, V. M. Chertok, and A. E. Kotsyuba, “Nitroxidergic neurons in the medulla oblongata in normo- and hypertensive rats,” Byull. Eksperim. Biol., 147, No. 8, 157–160 (2009).Google Scholar
- 3.A. E. Kotsyuba, V. M. Chertok, and E. V. Babich, “Nitroxidergic neurons in the bulbar vasomotor center in humans with arterial hypertension,” Zh. Nevrol. Psikhiat., No. 2, 61–65 (2010).Google Scholar
- 4.S. D. Mikhailova, “The involvement of serotonin in forming the activity of the bulbar cardiovascular center,” Vestn. Ross. Gos. Med. Univ., No. 1, 68–70 (2009).Google Scholar
- 5.V. A. Tsyrlin and R. S. Khrustaleva, “The role of the brainstem and spinal cord adrenergic mechanisms in the central regulation of the circulation,” Vestn. Aritmol., 22, 75–80 (2001).Google Scholar
- 6.V. M. Chertok and A. E. Kotsyuba, Structural Organization of the Bulbar Component of the Cardiovascular Center, Meditsina DV, Vladivostok (2013).Google Scholar
- 7.V. M. Chertok and A. E. Kotsyuba, “Distribution of NADPHdiaphorase and neuronal NO synthase in the nuclei of the medulla oblongata in rats,” Morfologiya, 144, No. 6, 9–14 (2013).Google Scholar
- 8.V. M. Chertok, A. E. Kotsyuba, and M. S. Startseva, “Use of the ‘pixel’ method in quantitative enzyme histochemistry,” Morfologiya, 142, No. 5, 71–75 (2012).Google Scholar
- 9.V. M. Chertok, A. E. Kotsyuba, and M. S. Startseva, “Use of a computer image superimposition method for topochemical mapping of neurons in the brain,” Tikhookean. Med. Zh., No. 3, 95–98 (2014).Google Scholar
- 12.A. Dahlstrom, “Evidence for the existence of monoamine containing neurons in the central nervous system,” Acta Physiol. Scand., 232, 1–15 (1964).Google Scholar
- 15.P. G. Guyenet, “Role of the ventral medulla oblongata in blood pressure regulation,” in: Central Regulation of Autonomic Functions, Oxford University Press, New York (1990), pp. 145–167.Google Scholar
- 21.K. P. Patel, Y.-F. Li, and Y. Hirooka, “Role of nitric oxide in central sympathetic outflow,” Exp. Biol. Med., 226, 814–824 (2001).Google Scholar