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Neuroscience and Behavioral Physiology

, Volume 46, Issue 1, pp 100–105 | Cite as

Developmental Characteristics of the Hypothalamo-Hypophyseal-Adrenal System in Chronic Heterotypical Stress

  • V. V. Khlebnikov
  • S. L. Kuznetsov
  • D. A. Chernov
  • A. M. Agrytskov
  • A. Ahmad
  • M. N. K. Nor-Ashikin
  • M. Ullah
  • M. Yu. Kapitonova
Article
  • 30 Downloads

Experimental studies of the developmental characteristics of the adaptation of the hypothalamo-hypophyseal-adrenal system (HHAS) to the actions of heterotypical stressors in the context of stress-associated behavioral reactions were studied. Sprague–Dawley rats aged three (young), six (adult), and 12 (aged) months (total number of animals = 36) were exposed to changing stressors for seven days, after which their behavioral reactions were assessed. Histological changes in the hypothalamus, hypophysis, and adrenals were studied and compared with age-matched controls, and immunochemical reactions to corticoliberin (CRF), adrenocorticotropic hormone (ACTH), ED1, PCNA, and caspase-3 were performed, with subsequent image analysis. In aged animals, as compared with young and adult animals, the levels of activation of the components of the HHAS provided evidence of dissociation in its central component and blockade of the adaptive desensitization typical of young and adult animals. In particular, aged animals showed a high level of CRF expression in the hypothalamus in stress, with a relatively low level of ACTH expression in the adenohypophysis and a high level of adrenal activity. Decreases in HHAS plasticity in aged rats as compared with other age groups corresponded to behavioral changes seen in these animals, demonstrating decreases in the ability of aged animals to adapt to chronic exposure to unpredictably changing stressors.

Keywords

hypothalamo-hypophyseal-adrenal system aging immunohistochemistry heterotopic stressor chronic stress 

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References

  1. 1.
    N. D. Goncharov, A. V. Shamlii, V. Yu. Marenin, and S. A. Smelkova, “The hypothalamo-hypophyseal-adrenal system and the enzymes of the glutathione-dependent antioxidant system in aging and stress,” Byull. Eksperim. Biol., 144, No. 7, 574–577 (2007).Google Scholar
  2. 2.
    M. Yu. Kapitonova, M. Ullah, S. L. Kuznetsov, et al., “Developmental morphofunctional chromosomes of folliculostellate cells in the rat hypophysis in stress,” Vestn. Ros. Akad. Med. Nauk, 11, 98–103 (2013).CrossRefGoogle Scholar
  3. 3.
    S. K. Sudakov, G. A. Nazarova, E. V. Alekseeva, and V. G. Bashkatova, “Identification of levels of anxiety in rats: divergence of results in the open field, plus maze, and Vogel tests,” Byull. Eksperim. Biol., 155, No. 3, 268–270 (2013).Google Scholar
  4. 4.
    V. G. Shalyapina, V. V. Rakitskaya, and E. I. Petrova, “The role of corticotropin-releasing hormone in behavioral disorders after unavoidable stress in active and passive rats,” Zh. Vyssh. Nerv. Deyat., No. 2, 241–246 (2005).Google Scholar
  5. 5.
    A. Armario, R. M. Escorihuela, and R. Nadal, “Long-term neuroendocrine and behavioural effects of a single exposure to stress in adult animals,” Neurosci. Behav. Rev., 32, 1121–1135 (2008).CrossRefGoogle Scholar
  6. 6.
    A. Avital, E. Ram, R. Maayan, et al., “Effects of early-life stress on behavior and neurosteroid levels in the rat hypothalamus and entorhinal cortex,” Brain Res. Bull., 68, 419–424 (2006).CrossRefPubMedGoogle Scholar
  7. 7.
    S. H. Booij, E. M. Bouma, P. De Jong, et al., “Chronicity of depressive problems and the cortisol response to psycho-social stress in adolescents: the TRAILS study,” Psychoneuroendocrinology, 38, No. 5, 659–666 (2013).CrossRefPubMedGoogle Scholar
  8. 8.
    B. K. Choudhury, X. Z. Shi, and S. K. Sarna, “Norepinephrine mediates the transcriptional effects of heterotypic chronic stress on colonic motor function,” Am. J. Physiol. Gastrointest. Liver Physiol., 296, No. 6, 1238–1247 (2009).CrossRefGoogle Scholar
  9. 9.
    G. Dagyte, E. A. Van der Zee, F. Postema, et al., “Chronic but not acute foot-shock stress leads to temporary suppression of cell proliferation in rat hippocampus,” Neuroscience, 162, No. 4, 904–913 (2009).CrossRefPubMedGoogle Scholar
  10. 10.
    A. Gądek-Michalska, J. Spyrka, P. Rachwalska, et al., “Influence of chronic stress on brain corticosteroid receptors and HPA axis activity,” Pharmacol. Rep., 65, No. 5, 1163–1175 (2013).CrossRefPubMedGoogle Scholar
  11. 11.
    A. Martocchia, M. Stefanelli, G. M. Falaschi, et al., “Targets of antiglucocorticoid therapy for stress-related diseases,” Recent Pat. CNS Drug Discov., 8, No. 1, 79–87 (2013).CrossRefPubMedGoogle Scholar
  12. 12.
    V. Mironova, E. Rybnikova, and S. Pivina, “Effect of inescapable stress in rodent models of depression and posttraumatic stress disorder on CRH and vasopressin immunoreactivity in the hypothalamic paraventricular nucleus,” Acta Physiol. Hung., 100, No. 4, 395–410 (2013).CrossRefPubMedGoogle Scholar
  13. 13.
    T. M. Segar, J. W. Kasckow, J. A. Welge, and J. P. Herman, “Hete rogeneity of neuroendocrine stress responses in aging rat strains II,” Physiol. Behav., 96, No. 1, 6–11 (2009).PubMedCentralCrossRefPubMedGoogle Scholar
  14. 14.
    M. B. Solomon, K. Jones, B. A. Packard, and J. P. Herman, “The medial amygdala modulates body weight but not neuroendocrine responses to chronic stress,” J. Neuroendocrinol., 22, No. 1, 13–23 (2010).CrossRefPubMedGoogle Scholar
  15. 15.
    I. Zalachoras, R. Hottman, E. Atucha, et al., “Differential targeting of brain stress circuits with a selective glucocorticoid receptor modulator,” Proc. Natl. Acad. Sci. USA, 110, No. 19, 7910–7915 (2013).PubMedCentralCrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • V. V. Khlebnikov
    • 1
    • 2
  • S. L. Kuznetsov
    • 3
  • D. A. Chernov
    • 3
  • A. M. Agrytskov
    • 2
  • A. Ahmad
    • 4
  • M. N. K. Nor-Ashikin
    • 4
  • M. Ullah
    • 5
  • M. Yu. Kapitonova
    • 4
  1. 1.Department of Internal Diseases, Faculty of MedicineMalaysian International Islamic UniversityKuantanMalaysia
  2. 2.Department of NeurologyVolgograd State Medical UniversityVolgogradRussia
  3. 3.Department of Histology, Embryology, and CytologySechenov First Moscow State Medical UniversityMoscowRussia
  4. 4.Department of Anatomy, Faculty of MedicineMARA University of TechnologyShah AlamMalaysia
  5. 5.Department of Anatomy, Faculty of DentistryMARA University of TechnologyShah AlamMalaysia

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