Effects of Dynorphin A (1-13) on Lipid Peroxidation and the Activity of Antioxidant Enzymes in Immobilization Stress of Different Durations
Experiments on rats showed that the selective opioid κ receptor agonist dynorphin A (1-13) had marked antioxidant actions in immobilization stress of different durations, apparent as reductions in lipid peroxidation products in liver tissues. The effects of this peptide on the activities of the antioxidant defense enzymes superoxide dismutase and catalase depended on the duration of stress. While the peptide had a notable stimulatory effect on catalase activity in animals subjected to immobilization for 3 h, this effect was significantly smaller in rats exposed to stress for 6 or 12 h. These studies demonstrated that dynorphin A (1-13) has a stimulatory action on superoxide dismutase activity in animals exposed to immobilization for 6 or 12 h, this being seen at all study time points. Peptide had no effect on the activity of this enzyme in rats exposed to immobilization stress for 3 h.
Keywordsstress lipid peroxidation antioxidant enzymes dynorphin
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- 2.I. S. Vyborova, Udval Khandzhav, L. S. Vasil’eva, and N. G. Makarova, “Liver structure during immobilization stress,” Sib. Med. Zh., No. 3, 30–33 (2005).Google Scholar
- 5.I. A. Zborovskaya and M. V. Bannikova, “The antioxidant system of the body, its signifi cance in metabolism, and clinical aspects,” Vestn. Ros. Akad. Med. Nauk., No. 6, 53–60 (1995).Google Scholar
- 6.A. V. Zor’kina, V. I. Inchina, and Ya. V. Kostin, “The antioxidant actions of cytochrome c in conditions of prolonged immobilization stress,” Byull. Eksperim. Biol. Med., 123, No. 6, 642–644 (1997).Google Scholar
- 7.M. A. Korolyuk, L. I. Ivanova, I. G. Maiorova, and V. E. Tokarev, “A method for assay of catalase activity,” Lab. Delo, No. 1, 216–19 (1988).Google Scholar
- 8.Yu. B. Lishmanov and L. N. Maslov, Opioid Neuropeptides, Stress, and Adaptive Protection of the Heart, Tomsk University Press, Tomsk (1994).Google Scholar
- 9.E. V. Makarenko, “Complex assay of superoxide dismutase and glutathione reductase activities in erythrocytes of patients with chronic liver diseases,” Lab. Delo, No. 11, 48–50 (1988).Google Scholar
- 10.A. P. Simonenkov and V. D. Fedorov, “The current concept of stress and adaption, with recognition of new data on the origin of tissue hypoxia,” Vestn. Ros. Akad. Med. Nauk., No. 5, 7–14 (2008).Google Scholar
- 11.A. V. Solin and Yu. D. Lyashev, “Effects of opioid peptides on levels of LPO products and the activity of the antioxidant system in the liver of rats subjected to immobilization stress,” Byull. Eksperim. Biol. Med., 153, No. 6, 803–805 (2012).Google Scholar
- 12.A. V. Solin and Yu. D. Lyashev, “Lipid peroxidation in immobilization stress of different durations,” Ros. Fiziol. Zh., 99, No. 6, 751–7556 (2013).Google Scholar
- 14.A. Negre-Salvayre, N. Aude, V. Ayala, et al., “Pathological aspects of lipid peroxidation,” Free Radic. Res., 48, No. 10, 2747–2753 (2010).Google Scholar
- 17.A. Wigger, P. Lorscher, I. Oehler, et al., “Nonresponsiveness of the rat hypothalamo-pituitary-adrenocortical axis to parturition-related events: inhibitory action of endogenous opioids,” Endocrinol., 140, No. 6, 2843–2849 (1999).Google Scholar