Neuroscience and Behavioral Physiology

, Volume 45, Issue 5, pp 490–492 | Cite as

Distribution of GABAergic Neurons in the Neocortex in Rats during the Postnatal Period after Perinatal Hypoxia

  • L. I. Khozhai
  • V. A. Otellin

The distribution of GABAergic neurons in different areas of the neocortex (frontal, sensorimotor, and visual) was studied in Wistar rats at different time points in the postnatal period of development after exposure to perinatal hypoxia. These neurons were detected using antibodies to GAD-67, a marker for GABAergic neurons. Perinatal hypoxia was found to lead to significant decreases in the numbers of GAD-67-expressing neurons in both the upper and the deep layers of the cortex at juvenile age (at day 20 of postnatal development), persisting to the pubertal period (day 40). The number of GAD-67-synthesizing neurons in each of the neocortical layers in experimental animals on day 40 were half those in controls. The sharp decrease in the number of GABAergic neurons in the neocortex is probably due to the harmful action of acute perinatal hypoxia on precursor cell migration from the subventricular zone or on the synthesis of the factors controlling these migration processes and the maturation of GABAergic neurons, and thus decreasing the late expression of GAD-67.


neocortex GABAergic neurons perinatal hypoxia 


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  1. 1.
    V. A. Otellin, L. I. Khozhai, L. A. Vataeva, and T. T. Shishko, “Longterm consequences of hypoxia during the prenatal period of development on the structural-functional characteristics of the brain in rats,” Ros. Fiziol. Zh., 97, No. 10, 1092–1100 (2011).Google Scholar
  2. 2.
    V. A. Otellin, L. I. Khozhai, and L. A. Vataeva, “The effects of hypoxia in early perinatal ontogeny on behavior and the structural characteristics of the brain,” Zh. Evolyuts. Biokhim., 46, No. 5, 467–473 (2012).Google Scholar
  3. 3.
    V. A. Otellin, L. I. Khozhai, and T. T. Shishko, “Responses of the nerve elements of the neocortex on the effects of hypoxia during the early neonatal period in rats,” Zh. Evolyuts. Biokhim., 50, No. 2, 148–154 (2014).Google Scholar
  4. 4.
    A. J. Bolteus and A. Bordey, “GABA release and uptake regulate neuronal precursor migration in the postnatal subventricular zone,” J. Neurosci., 24, 7623–7631 (2004).CrossRefPubMedGoogle Scholar
  5. 5.
    D. L. Kaufman, C. R. Houser, and A. J. Tobin, “Two forms of the gamma-aminobutyric acid synthetic enzyme of glutamate decarboxylase have distinct interneuronal distribution and cofactor interactions,” J. Neurochem., 56, No. 2, 720–723 (1991).CrossRefPubMedGoogle Scholar
  6. 6.
    C. Le Magueresse, J. Alfonso, K. Khodosevich, et al., “‘Small axonless neuron:’ postnatally generated neocortical interneurons with delayed functional maturation,” J. Neurosci., 31, No. 46, 16731–16747 (2011).CrossRefPubMedGoogle Scholar
  7. 7.
    O. Marín and J. L. Rubenstain, “Cell migration in the forebrain,” Annu. Rev. Neurosci., 26, 441–43 (2003).CrossRefPubMedGoogle Scholar

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© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Laboratory for the Ontogeny of the Nervous System, Pavlov Institute of PhysiologyRussian Academy of SciencesSt. PetersburgRussia

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