Neuroscience and Behavioral Physiology

, Volume 44, Issue 5, pp 542–545 | Cite as

Advantages and Disadvantages of Zinc-Ethanol-Formaldehyde as a Fixative for Immunocytochemical Studies and Confocal Laser Microscopy

  • D. E. Korzhevskii
  • E. G. Sukhorukova
  • E. G. Gilerovich
  • E. S. Petrova
  • O. V. Kirik
  • I. P. Grigor’ev

We present here an analysis of our own results obtained by fixing various tissues in zinc-ethanol-formaldehyde (ZEF). As compared with other fixation methods, fixation in ZEF was found to provide greater sensitivity for immunocytochemical reactions for many study antigens, in many cases also allowing thermal demasking of antigens to be avoided and giving higher image quality using fluorescence and confocal laser microscopy. However, studies of low molecular weight antigens showed diffusion of antigens from their initial locations. These data lead to the conclusion that fixation of specimens in ZEF has potential for use both in immunocytochemical studies, including use of fluorescence and confocal laser microscopy, and in general histological practice.


immunocytochemistry fixation methods confocal laser microscopy 


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • D. E. Korzhevskii
    • 1
  • E. G. Sukhorukova
    • 1
  • E. G. Gilerovich
    • 1
  • E. S. Petrova
    • 1
  • O. V. Kirik
    • 1
  • I. P. Grigor’ev
    • 1
  1. 1.Laboratory for the Functional Morphology of the Central and Peripheral Nervous System, Department of General and Special Morphology, Research Institute of Experimental Medicine, North-West BranchRussian Academy of Medical SciencesSt. PetersburgRussia

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