Skip to main content
SpringerLink
Log in

Development of Dissociated Cells from Different CNS Rudiments in Rats after Transplantation into Injured Nerve

  • Published:
Neuroscience and Behavioral Physiology Aims and scope Submit manuscript

The aim of the present work was to identify the potential for implantation of dissociated embryonic spinal cord and neocortex rudiment cells into the sciatic nerve of Wistar rats and to study their differentiation. Cell suspensions prepared by dissociation of fragments of the cervical segment of the spinal cord and anterior cerebral vesicle of rat fetuses at 15 days of development were injected into the proximal segment of previously injured sciatic nerve. Studies using the immunohistochemical marker for stem/progenitor cells (Msi-1) showed that implanted cells could be identified in nerve trunks one day after surgery. At 21 days, some were fully differentiated into NeuN-immunopositive neurons, though the numbers of these cells were small. Most cells in neocortical transplants, in contrast to those of spinal cord transplants, were ependymocytes at 21 days after surgery.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. M. N. Karagyaur, T. V. Lopatina, D. V. Stambolskii, et al., “Mesenchymal stem cells stimulate recovery of peripheral nerves due to secretion of neurotrophic factors,” in: Proc. All-Russ. Conf. Regenerative Biology and Medicine, Narkonet Press, Moscow (2011), pp. 78–79.

    Google Scholar 

  2. D. E. Korzhevskii, M. M. Lentsman, O. V. Kirik, and V. A. Otellin, “Vimentin-immunopositive cells in the rat telencephalon after experimental ischemic stroke,” Morfologiya, 132, No. 5, 23–27 (2007).

    CAS  Google Scholar 

  3. E. S. Petrova, “Use of stem cells to stimulate the regeneration of injured nerves,” Tsitologiya, 54, No. 7, 525–540 (2012).

    CAS  Google Scholar 

  4. E. S. Petrova and V. A. Otellin, “Characteristics of the development of homo- and heterotopic allotransplants of embryonic rat neocortex,” Tsitologiya, 42, No. 8, 750–757 (2000).

    CAS  Google Scholar 

  5. K. K. Sukhinich, O. V. Podgornyi, and M. A. Aleksandrova, “Immunocytochemical analysis of the development of suspension and tissue neurotransplants,” Izv. Ros. Akad. Nauk. Ser. Biol., No. 6,659–668 (2011).

    Google Scholar 

  6. Yu. A. Chelyshev, Regeneration in the Nervous System: Guidelines for Histology [in Russian], SpetsLit, St. Petersburg (2011), Vol. 1, pp. 656–665.

    Google Scholar 

  7. E. I. Chumasov and E. S. Petrova, “Implantation of embryonic neocortical and spinal cord rudiments into injured peripheral nerves in adult rats,” Byull. Eksperim. Biol., 108, No. 8, 198–201 (1990).

    Google Scholar 

  8. R. V. Bellamkonda, “Peripheral nerve regeneration: an opinion on channels, scaffolds and anisotropy,” Biomaterials, 27, No. 19, 3515–3518 (2006).

    CAS  PubMed  Google Scholar 

  9. I. Bystron, V. A. Otellin, T. Wierzba-Bobrowicz, and J. Dymecki, “Development of human fetal substantia nigra grafts in the brain of non-immunosuppressed rats,” Folia Neuropathol., 35, No. 2, 87–93 (1997).

    CAS  PubMed  Google Scholar 

  10. C. Cheng and D. W. Zochodne, “In vivo proliferation, migration and phenotypic changes of Schwann cells in the presence of myelinated fibers,” Neurosci., 115, No. 1, 321–329 (2002).

    Article  CAS  Google Scholar 

  11. A. K. Chojnacki, G. K. Mak, and S. Weiss, “Identity crisis for adult periventricular neural stem cells: subventricular zone astrocytes, ependymal cells or both?” Nat. Rev. Neurosci., 10,. No. 4, 153–163 (2009).

    Article  CAS  PubMed  Google Scholar 

  12. S. Geuna, S. Nicolino, S. Raimondo, et al., “Nerve regeneration along bioengineered scaffolds,” Microsurgery, 27, No. 5, 429–438 (2007).

    Article  CAS  PubMed  Google Scholar 

  13. J. Mokry and J. Karbanová, “Foetal mouse neural stem cells give rise to ependymal cells in vitro,” Folia Biol (Praha), 52, No. 5, 149–155 (2006).

    CAS  Google Scholar 

  14. S. Walsh and R. Midha, “Use of stem cells to augment nerve injury repair,” Neurosurgery, 65, No. 4, 80–86 (2009).

    Article  Google Scholar 

  15. G. Xiong, N. Ozaki, and Y. Sugiura, “Transplanted embryonic spinal tissue promotes severed sciatic nerve regeneration in rats,” Archaeological. Histol. Cytol., 72, No. 2, 127–138(2009).

    Article  Google Scholar 

  16. W. M. Zawada, D. J. Zastrow, E. D. Clarkson, et al., “Growth factors improve immediate survival of embryonic dopamine neurons after transplantation into rats,” Brain Res., 786, No. 1–2, 96–103 (1998).

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Central and Peripheral Nervous System Functional Morphology Laboratory, Department of General and Special Morphology, Research Institute of Experimental Medicine, North-West Branch, Russian Academy of Medical Sciences, 12 Academician Pavlov Street, 197376, St. Petersburg, Russia

    E. S. Petrova & D. E. Korzhevskii

  2. Immunopharmacology Laboratory, Research Institute of Specially Pure Biological Preparations, 7 Pudozhskaya Street, 197110, St. Petersburg, Russia

    E. N. Isaeva

Authors
  1. E. S. Petrova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. N. Isaeva
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. E. Korzhevskii
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to D. E. Korzhevskii.

Additional information

Translated from Morfologiya, Vol. 143, No. 2, pp. 30–34, March–April, 2013.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Petrova, E.S., Isaeva, E.N. & Korzhevskii, D.E. Development of Dissociated Cells from Different CNS Rudiments in Rats after Transplantation into Injured Nerve. Neurosci Behav Physi 44, 478–481 (2014). https://doi.org/10.1007/s11055-014-9936-z

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11055-014-9936-z

Keywords

Search

Navigation