Development of Dissociated Cells from Different CNS Rudiments in Rats after Transplantation into Injured Nerve
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The aim of the present work was to identify the potential for implantation of dissociated embryonic spinal cord and neocortex rudiment cells into the sciatic nerve of Wistar rats and to study their differentiation. Cell suspensions prepared by dissociation of fragments of the cervical segment of the spinal cord and anterior cerebral vesicle of rat fetuses at 15 days of development were injected into the proximal segment of previously injured sciatic nerve. Studies using the immunohistochemical marker for stem/progenitor cells (Msi-1) showed that implanted cells could be identified in nerve trunks one day after surgery. At 21 days, some were fully differentiated into NeuN-immunopositive neurons, though the numbers of these cells were small. Most cells in neocortical transplants, in contrast to those of spinal cord transplants, were ependymocytes at 21 days after surgery.
Keywordsnerve regeneration neural stem/progenitor cells ependymocytes
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