Neuroscience and Behavioral Physiology

, Volume 42, Issue 1, pp 89–96 | Cite as

Contribution of Glucocorticoid Hormones to the Gastroprotective Effect of Ischemic Preconditioning in Rats in Normal Conditions and In Prostaglandin Deficiency

  • P. Yu. Bobryshev
  • T. R. Bagaeva
  • L. P. Filaretova

The aim of the present work was to study the contribution of glucocorticoid hormones to the gastroprotective effects of ischemic preconditioning in a gastric ischemia/reperfusion model in rats with normal and deficient prostaglandin production. The contribution of glucocorticoids was evaluated by adrenalectomy followed by corticosterone replacement therapy and transient inhibition of corticosterone synthesis with metyrapone. Prostaglandin synthesis was blocked with indomethacin. Gastric ischemia was created by compression of the celiac artery in anesthetized rats. Ischemic preconditioning (0.5 h ischemia/reperfusion) stimulated corticosterone production and decreased the formation of gastric erosions due to 3.5 h ischemia/reperfusion. Adrenalectomy and administration of metyrapone prevented the gastroprotective effect of ischemic preconditioning on the backgrounds of both normal and deficient prostaglandin production. Corticosterone replacement therapy restored the effect of ischemic preconditioning in adrenalectomized rats, even in conditions of blockade of prostaglandin synthesis. Thus, glucocorticoid hormones make a contribution to the gastroprotective effect of ischemic preconditioning in conditions of normal prostaglandin production, as well as in conditions of blockade of prostaglandin synthesis.


ischemic preconditioning ischemia/reperfusion stomach erosions glucocorticoid hormones gastroprotection rats 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    P. Yu. Bobryshev, T. R. Bagaeva, and L. P. Filaretova, “Involvement of glucocorticoid hormones in protecting the gastric mucosa in ischemia and ischemia/reperfusion,” Ros. Fiziol. Zh. im. I. M. Sechenova, 95, No. 11, 1225–1233 (2009).Google Scholar
  2. 2.
    L. P. Filaretova, “Stress and ulcer formation in the stomach: the gastroprotective role of glucocorticoid hormones,” Ros. Fiziol. Zh. im. I. M. Sechenova, 95, No. 10, 1160–1170 (2009).Google Scholar
  3. 3.
    P. Bobryshev, N. Podvigina, N. Maltcev, and L. Filaretova, “Gastric microcirculation as target of gastroprotective action of glucocorticoid hormones in rats with desensitization of capsaicin-sensitive neurons,” Inflammopharmacology, 14, No. 5–6, 236–242 (2006).PubMedCrossRefGoogle Scholar
  4. 4.
    T. Brzozowski, P. C. Konturek, J. J. Konturek, R. Pajdo, S. Kwiecien, M. Pawlik, D. Drozdowicz, Z. Sliwowski, and W. W. Pawlik, “Ischemic preconditioning of remote organs attenuates gastric ischemia-reperfusion injury through involvement of prostaglandins and sensory nerves,” Eur. J. Pharmacol., 499, No. 1–2, 201–213 (2004).PubMedCrossRefGoogle Scholar
  5. 5.
    T. Brzozowski, P. C. Konturek, S. J. Konturek, I. Brzozowska, and T. Pawlik, “Role of prostaglandins in gastroprotection and gastric adaptation,” J. Physiol. Pharmacol., 56, No. 5, 33–55 (2005).PubMedGoogle Scholar
  6. 6.
    L. P. Filaretova, “The hypothalamic-adrenocortical system: hormonal brain-gut interaction and gastroprotection,” Auton. Neurosci., 125, No. 1–2, 86–93 (2006).PubMedCrossRefGoogle Scholar
  7. 7.
    L. P. Filaretova, A. A. Filaretov, and G. B. Makara, “Corticosterone increase inhibits stress-induced gastric erosion in rats,” Am. J. Physiol., 274, No. 37, G1024–G1030 (1998).PubMedGoogle Scholar
  8. 8.
    L. Filaretova, T. Bagaeva, K. Amagase, and K. Takeuchi, “Contribution of glucocorticoids to protective influence of preconditioning mild stress against stress-induced gastric erosions,” Ann. N.Y. Acad. Sci., 1148, 209–212 (2008).PubMedCrossRefGoogle Scholar
  9. 9.
    L. Filaretova, M. Maltcev, A. Bogdanov, and Y. Levkovich, “Role of gastric microcirculation in the gastroprotection by glucocorticoids released during water-restraint stress in rats,” Chin. J. Physiol., 42, No. 3, 145–152 (1999).PubMedGoogle Scholar
  10. 10.
    L. Filaretova, A. Tanaka, Y. Komoike, and K. Takeuchi, “Selective cyclooxygenase inhibitor induces gastric mucosal damage in adrenalectomized rats,” Inflammopharmacology, 10, No. 4–6, 413–422 (2002).CrossRefGoogle Scholar
  11. 11.
    E. Mikics, M. R. Kruk, and J. Haller, “Genomic and non-genomic effects of glucocorticoids on aggressive behavior in male rats,” Psychoneuroendocrinology, 29, No. 5, 618–635 (2004).PubMedCrossRefGoogle Scholar
  12. 12.
    C. E. Murry, R. B. Jennings, and K. A. Reimer, “Preconditioning with ischemia: a delay of lethal cell injury in ischemic myocardium,” Circulation, 74, No. 5, 1124–1136 (1986).PubMedCrossRefGoogle Scholar
  13. 13.
    R. Pajdo, T. Brzozowski, P. C. Konturek, S. Kwiecien, S. J. Konturek, Z. Sliwowski, M. Pawlik, A. Ptak, D. Drozdowicz, and E. G. Hahn, “Ischemic preconditioning, the most effective gastroprotective intervention: involvement of prostaglandins, nitric oxide, adenosine and sensory nerves,” Eur. J. Pharmacol., 427, No. 3, 263–276 (2001).PubMedCrossRefGoogle Scholar
  14. 14.
    J. Parratt and A. Vegh, “Pronounced antiarrhythmic effects of ischemic preconditioning,” Cardioscience, 5, No. 1, 9–18 (1994).PubMedGoogle Scholar
  15. 15.
    A. Robert, J. E. Nezamis, C. Lancaster, J. P. Davis, S. O. Field, and A. J. Hanchar, “Mild irritants prevent gastric necrosis through ‘adaptive cytoprotection’ mediated by prostaglandins,” Am. J. Physiol., 245, No. 1, G113–G121 (1983).PubMedGoogle Scholar
  16. 16.
    K. Takeuchi, H. Araki, M. Umeda, Y. Komoike, and K. Suzuki, “Adaptive gastric cytoprotection is mediated by prostaglandin EP1 receptors: a study using rats and knockout mice,” J. Pharmacol. Exp. Ther., 297, No. 3, 1160–1165 (2001).PubMedGoogle Scholar
  17. 17.
    A. Tanaka, R. Hatazawa, Y. Takahira, N. Izumi, L. Filaretova, and K. Takeuchi, “Preconditioning stress prevents cold restraint stressinduced gastric lesions in rats: roles of COX-1, COX-2, and PLA2,” Dig. Dis. Sci., 52, No. 2, 478–487 (2007).PubMedCrossRefGoogle Scholar
  18. 18.
    K. Tanaka, K. Nishimoto, W. Tomisato, S. Tsutsumi, T. Hoshino, T. Tsuchiya, and T. Mizushima, “Adaptive cytoprotection induced by pretreatment with ethanol protects against gastric cell damage by NSAIDs,” Dig. Dis. Sci., 49, No. 2, 210–217 (2004).PubMedCrossRefGoogle Scholar
  19. 19.
    K. Wada, Y. Kamisaki, M. Kitano, Y. Kishimoto, K. Nakamoto, and T. Itoh, “A new gastric ulcer model induced by ischemia-reperfusion in the rat: role of leukocytes on ulceration in rat stomach,” Life Sci., 59, No. 19, PL295–PL301 (1996).CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, Inc. 2011

Authors and Affiliations

  • P. Yu. Bobryshev
    • 1
  • T. R. Bagaeva
    • 1
  • L. P. Filaretova
    • 1
  1. 1.Experimental Endocrinology Laboratory, I. P. Pavlov Institute of PhysiologyRussian Academy of SciencesSt. PetersburgRussia

Personalised recommendations