Neuroscience and Behavioral Physiology

, Volume 41, Issue 3, pp 228–232 | Cite as

Transcriptional Activity of Nuclear Factor Kappa B (NF-κB) in Post-Traumatic Sensory Neurons (a histochemical study)

  • S. V. Gushchina
  • O. V. Volkova
  • P. P. Kruglyakov
  • C. B. Magoulas

Nuclear factor kappa B (NF-κB) is a transcription factor that regulates the expression of a large set of genes involved in immune and inflammatory processes. It has been suggested that the release of specific cytokines following nerve damage has a stimulatory action, activating NF-κB in neurons in the spinal ganglia (SG), which may have protective influences on sensory neurons. However, the complexity of this factor has led to contradictory conclusions on the role of NF-κB in damaged SG neurons. The aim of the present work was to determine whether NF-κB is activated in sensory neurons in mature SG in conditions of peripheral nerve damage using transgenic reporter mice in which activation of NF-κB leads to expression of the lac-z reporter gene. These experiments showed that expression of β-galactosidase (β-gal) did not occur either in damaged SG neurons or in neurons on the contralateral side. However, powerful β-gal expression was seen in damaged muscle tissue. This may reflect the regressive influence of additional signal molecules on NF-κB activity in sensory neurons.

Key words

neuron transcription factor NF-κB spinal ganglia reporter gene 


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  1. 1.
    Yu. A. Chelyshev, “Factors maintaining the regeneration of peripheral nerve conductors,” Usp. Fiziol. Nauk., 26, No. 2, 57–77 (1995).PubMedGoogle Scholar
  2. 2.
    B. P. Ashburner, S. D. Westerheide, and A. S. Baldwin, Jr., “The p65 (RelA) subunit of NF-kappaB interacts with the histone deacetylase (HDAC) corepressors HDAC1 and HDAC2 to negatively regulate gene expression,” Mol. Cell. Biol., 21, No. 20, 7065–7077 (2001).PubMedCrossRefGoogle Scholar
  3. 3.
    A. S. Baldwin, Jr., “The NF-kappa B and I kappa B proteins: new discoveries and insights,” Annu. Rev. Immunol., No. 14, 649–683 (1996).Google Scholar
  4. 4.
    A. L. Bhakar, L. L. Tannis, C. Zeindler, et al., “Constitutive nuclear factor-kappa B activity is required for central neuron survival,” J. Neurosci., 22, No. 19, 8466–8475 (2002).PubMedGoogle Scholar
  5. 5.
    H. Carlsen, J. O. Moskaug, S. H. Fromm, et al., “In vivo imaging of NF-kappa B activity,” J. Immunol., 168, No. 3, 1441–1446 (2002).PubMedGoogle Scholar
  6. 6.
    J. J. Inglis, A. Nissim, D. M. Lees, et al., “The differential contribution of tumour necrosis factor to thermal and mechanical hyperalgesia during chronic inflammation,” Arthritis Res. Ther., 7, No. 4, 807–816 (2005).CrossRefGoogle Scholar
  7. 7.
    W. Ma and M. A. Bisby, “Increased activation of nuclear factor kappa B in rat lumbar dorsal root ganglion neurons following partial sciatic nerve injuries,” Brain Res., 797, No. 2, 243–254 (1998).PubMedCrossRefGoogle Scholar
  8. 8.
    S. Mehmet, “NF-kappaB functions in the nervous system: from development to disease,” Biochem. Pharmacol., 72, No. 9, 1180–1195 (2006).CrossRefGoogle Scholar
  9. 9.
    P. G. Murphy, L. S. Borthwick, R. A. Johnston, et al., “Nature of the retrograde signal from injured nerves that induces interleukin-6 mRNA in neurons,” J. Neurosci., 19, No. 10, 3791–3800 (1999).PubMedGoogle Scholar
  10. 10.
    H. L. Pahl, “Activators and target genes of Rel/NF-kappaB transcription factors,” Oncogene, 18, No. 49, 6853–6866 (1999).PubMedCrossRefGoogle Scholar
  11. 11.
    G. Pollock, K. R. Pennypacker, S. Memet, et al., “Activation of NF-kappaB in the mouse spinal cord following sciatic nerve transection,” Exp. Brain Res., 165, No. 4, 470–477 (2005).PubMedCrossRefGoogle Scholar
  12. 12.
    G. Raivich and M. Makwana, “The making of successful axonal regeneration: genes, molecules and signal transduction pathways,” Brain Res. Rev., 53, No. 2, 287–311 (2007).PubMedCrossRefGoogle Scholar
  13. 13.
    R. N. Saha, M. Jana, K. Pahan, et al., “MAPK p38 regulates transcriptional activity of NF-kappaB in primary human astrocytes via acetylation of p65,” J. Immunol., 179, No. 10, 7101–7109 (2007).PubMedGoogle Scholar
  14. 14.
    R. Schmidt-Ullrich, S. Memet, A. Lilienbaum, et al., “NF-kappaB activity in transgenic mice: developmental regulation and tissue specificity,” Development, 122, No. 7, 2117–2128 (1996).PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, Inc. 2011

Authors and Affiliations

  • S. V. Gushchina
    • 1
  • O. V. Volkova
    • 1
  • P. P. Kruglyakov
    • 2
  • C. B. Magoulas
    • 3
  1. 1.Department of Histology and Embryology (Director: Academician of the Russian Academy of Medical Sciences Professor O. V. Volkova), Faculty of PediatricsRussian State Medical UniversityMoscowRussia
  2. 2.Department of Histology, Cytology, and Embryology (Director: Corresponding Member of the Russian Academy of Medical Sciences Prof. S. L. Kuznetsov)Sechenov Moscow Medical AcademyMoscowRussia
  3. 3.Neurosciences CenterUniversity of London (Director: Prof. G. Giovannoni)LondonEngland

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