Neuroscience and Behavioral Physiology

, Volume 40, Issue 5, pp 557–564 | Cite as

Characteristics and Interaction of GABAergic and Glycinergic Processes in Frog Spinal Cord Neurons

  • D. V. Amakhin
  • N. P. Veselkin

Whole-cell patch clamp recordings from isolated spinal cord neurons from the frog Rana temporaria were made to study the interaction of processes induced by application of GABA and glycine. The amplitudes of currents evoked by application of glycine did not change with time, while the amplitudes of GABAmediated currents decreased two-fold during the first 15 min of the experiment and stabilized at the new level. Neuron responses to simultaneous application of GABA and glycine were always smaller than the sum of the responses to separate application of these neurotransmitters. On application of GABA and glycine at the same concentration (5 mM), the amplitude of the response to simultaneous application decreased with time, reaching the level of the glycine-mediated response. A mixture of glycine and GABA at 8 μM and 5 mM, respectively, gave settled responses which were larger than the largest individual response by more than obtained with other mixtures. These data provide evidence that frog motoneurons may express receptors activated by both GABA and glycine.

Key words

GABA glycine score motoneuron patch clamp 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    N. I. Kalinina, G. G. Kurchavyi, D. V. Amakhin, and N. P. Veselkin, “Differences in the activation of inhibitory GABA and glycine motoneuron receptors in the frog Rana ridibunda and their interaction,” Ros. Fiziol. Zh. im. I. M. Sechenova, 94, No. 9, 1005–1016 (2008).Google Scholar
  2. 2.
    Yu. A. Polina, D. V. Amakhin,V. M. Kozhanov, G. G. Kurchavyi, and N. P. Veselkin, “Three types of inhibitory miniature potentials in frog spinal cord motoneurons: possible GABA and glycine co-transmission,” Ros. Fiziol. Zh. im. I. M. Sechenova, 92, No. 1, 18–26 (2006).Google Scholar
  3. 3.
    E. A. Tsvetkov and N. P. Veselkin, “Interaction of the postsynaptic effects of glycine and GABA in spinal cord neurons in the frog Rana temporaria,” Ros. Fiziol. Zh. im. I. M. Sechenova, 93, No. 7, 735–745 (2007).Google Scholar
  4. 4.
    K. V. Baev, K. I. Rusin, and B. V. Safronov, “Primary receptor for inhibitory transmitters in lamprey spinal cord neurons,” Neurosci., 46, No. 4, 931–941 (1992).CrossRefGoogle Scholar
  5. 5.
    Q. X. Chen, A. Stelzer, A. R. Kay, and R. K. S. Wong, “GABAA receptor function is regulated by phosphorylation in acutely dissociated guinea-pig hippocampal neurones,” J. Physiol., 420, 207–221 (1990).PubMedGoogle Scholar
  6. 6.
    S. De Saint Jan, B. David-Watine, H. Korn, and P. Bregetovski, “Activation of human αl and α2 homomeric glycine receptors by taurine and GABA,” J. Physiol., 535, No. 3, 741–755 (2001).CrossRefPubMedGoogle Scholar
  7. 7.
    S. Fucile, D. de Saint Jan, B. David-Watine, H. Korn, and P. Bregetovski, “Comparison of glycine and GABA actions on the zebrafish homomeric glycine receptor,” J. Physiol., 517, No. 2, 369–383 (1999).CrossRefPubMedGoogle Scholar
  8. 8.
    P. Jonas, J. Bischofberger, and J. Sandkuhler, “Corelease of two fast neurotransmitters at a central synapse,” Science, 281, 419–424 (1998).CrossRefPubMedGoogle Scholar
  9. 9.
    P. Li and M. Slaughter, “Glycine receptor subunit composition alters the action of GABA antagonists,” Vis. Neurosci., 24, 513–521 (2007).CrossRefPubMedGoogle Scholar
  10. 10.
    Y. Li, L.-J. Wu, P. Legendre, and T.-L. Xu, “Asymmetric cross-inhibition between GABAA and glycine receptors in rat spinal dorsal horn neurons,” J. Biol. Chem., 278, No. 40, 38637–38645 (2003).CrossRefPubMedGoogle Scholar
  11. 11.
    Y. Li and T.-L. Xu, “State-dependent cross-inhibition between anionic GABAA and glycine ionotropic receptors in rat hippocampal CAI neurons,” NeuroReport, 13, No. 2, 223–226 (2002).CrossRefPubMedGoogle Scholar
  12. 12.
    J. A. O’Brien and A. J. Berger, “Cotransmission of GABA and glycine to brain stem motoneurons,” J. Neurophysiol., 82, 1638–1641 (1999).PubMedGoogle Scholar
  13. 13.
    M. Russier, I. L. Kopysova, N. Ankri, N. Ferrand, and D. Debanne, “GABA and glycine core-lease optimizes functional inhibition in rat brainstem motoneurons in vitro,” J. Physiol., 541, No. 1, 123–137 (2002).CrossRefPubMedGoogle Scholar
  14. 14.
    T. Shirasaki, K. Aibara, and N. Akaike, “Direct modulation of GAB AA receptor by intracellular ATP in dissociated nucleus tractus solitarii neurons of rat,” J. Physiol., 449, 551–572 (1992).PubMedGoogle Scholar
  15. 15.
    P. Q. Trombley, B. J. Hill, and M. S. Horning, “Interactions between GABA and glycine at inhibitory amino acid receptors on rat olfactory bulb neurons,” J. Neurophysiol., 82, 3417–3422 (1999).PubMedGoogle Scholar
  16. 16.
    V. S. Vorobjov, I. N. Sharonova, and H. L. Haas, “A simple perfusion system for patchclamp studies,” J. Neurosci. Meth., 68, 303–307 (1996).CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, Inc. 2010

Authors and Affiliations

  1. 1.I. M. Sechenov Institute of Evolutionary Physiology and BiochemistryRussian Academy of SciencesSt. PetersburgRussia

Personalised recommendations