Neuroscience and Behavioral Physiology

, Volume 40, Issue 3, pp 263–267 | Cite as

Regulation of Food Consumption during Pregnancy and Lactation in Mice


The aim of the present work was to assess the expression of agouti-like protein and neuropeptide Y in pregnant and lactating mice and to compare this with the leptin level and food consumption. Food consumption, blood leptin levels, and agouti-like protein and neuropeptide Y mRNA levels in the hypothalamus of C57Bl/6J mice were assessed on days 7, 13, and 18 of pregnancy and on days 10 and 21 of lactation, and in virgin females of the same ages. During pregnancy, food consumption and leptin levels decreased on day 7 and increased in day 18 of pregnancy, while neuropeptide Y mRNA levels increased on day 13 and then remained unaltered, and the agouti-like protein level increased on day 18. After parturition, food consumption continued to increase, while leptin levels and neuropeptide Y mRNA levels decreased to normal. Thus, hyperphagia in pregnancy was due to sequential activation of the expression of neuropeptide Y and agouti-like protein, while in lactation hyperphagia resulted from mechanisms not associated with changes in the expression of these neuropeptides.

Key words

pregnancy lactation mice appetite leptin neuropeptide Y agouti-like protein 


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  1. 1.
    E. N. Makarova, A. Yu. Shevchenko, T. V. Yakovleva, and N. M. Bazhan, “Dynamics of food consumption and blood hormone levels in mice during pregnancy and lactation,” Ros. Fiziol. Zh. im. I. M. Sechenova, 93, No. 10, 1196–1205 (2007).Google Scholar
  2. 2.
    R. A. Augustine, S. R. Ladyman, and D. R. Grattan, “From feeding one to feeding many: hormone-induced changes in bodyweight homeostasis during pregnancy,” J. Physiol., 586, No. 2, 387–397 (2008).CrossRefPubMedGoogle Scholar
  3. 3.
    P. J. Brunton and J. A. Russell, “The expectant brain: adapting for motherhood,” Nat. Rev. Neurosci., 9, No. 1, 11–25 (2008).CrossRefPubMedGoogle Scholar
  4. 4.
    P. Chen, C. Li, C. Haskell-Luevano, R. D. Cone, and M. S. Smith, “Altered expression of agouti-related protein and its colocalization with neuropeptide Y in the arcuate nucleus of the hypothalamus during lactation,” Endocrinology, 140, No. 6, 2645–2650 (1999).CrossRefPubMedGoogle Scholar
  5. 5.
    P. Chen and M. S. Smith, “Regulation of hypothalamic neuropeptide Y messenger ribonucleic acid expression during lactation: role of prolactin,” Endocrinology, 145, No. 2, 823–829 (2004).CrossRefPubMedGoogle Scholar
  6. 6.
    W. R. Crowley, G. Ramoz, R. Torto, and S. P. Kalra, “Role of leptin in orexigenic neuropeptide expression during lactation in rats,” J. Neuroendocrinol., 16, No. 7, 637–644 (2004).CrossRefPubMedGoogle Scholar
  7. 7.
    W. R. Crowley, G. Ramoz, R. Torto, K. A. Keefe, J. J. Wang, and S. P. Kalra, “Neuroendocrine actions and regulation of hypothalamic neuropeptide Y during lactation,” Peptides, 28, No. 2, 447–452 (2007).CrossRefPubMedGoogle Scholar
  8. 8.
    M. Garcia, M. López, O. Gualillo, L. M. Soane, C. Diéguez, and R. M. Señarís, “Hypothalamic levels of NPY, MCH, and preproorexin mRNA during pregnancy and lactation in the rat: role of prolactin,” FASEB J., 17, No. 11, 1392–1400 (2003).CrossRefPubMedGoogle Scholar
  9. 9.
    M. D. Garcia, F. F. Casanueva, C. Diéguez, and R. M. Señarís, “Gestational profile of leptin messenger ribonucleic acid (mRNA) content in the placenta and adipose tissue in the rat, and regulation of the mRNA levels of the leptin receptor subtypes in the hypothalamus during pregnancy and lactation,” Biol. Reprod., 62, No. 3, 698–703 (2000).CrossRefPubMedGoogle Scholar
  10. 10.
    D. R. Grattan, S. R. Ladyman, and R. A. Augustine, “Hormonal induction of leptin resistance during pregnancy,” Physiol. Behav., 91, No. 4, 366–374 (2007).CrossRefPubMedGoogle Scholar
  11. 11.
    P. J. Havel, “Peripheral signals conveying metabolic information to the brain: short-term and long-term regulation of food intake and energy homeostasis,” Exp. Biol. Med. (Maywood), 226, No. 11, 963–977 (2001).Google Scholar
  12. 12.
    M. C. Henson and V. D. Castracane, “Leptin in pregnancy: an update,” Biol. Reprod., 74, No. 2, 218–229 (2006).CrossRefPubMedGoogle Scholar
  13. 13.
    J. W. Hill and J. E. Levine, “Abnormal response of the neuropeptide Y-deficient mouse reproductive axis to food deprivation but not lactation,” Endocrinology, 144, No. 5, 1780–1786 (2003).CrossRefPubMedGoogle Scholar
  14. 14.
    E. Jéquier, “Leptin signaling, adiposity, and energy balance,” Ann. N.Y. Acad. Sci., 967, 379–388 (2002).PubMedCrossRefGoogle Scholar
  15. 15.
    S. P. Kalra, M. G. Dube, S. Pu, B. Xu, T. L. Horvath, and P. S. Kalra, “Interacting appetite-regulating pathways in the hypothalamic regulation of body weight,” Endocr. Rev., 20, No. 1, 68–100 (1999).CrossRefPubMedGoogle Scholar
  16. 16.
    N. Kronfeld-Schor, J. Zhao, B. A. Silvia, E. Bicer, P. T. Mathews, R. Urban, S. Zimmerman, T. H. Kunz, and E. P. Widmaier, “Steroiddependent up-regulation of adipose leptin secretion in vitro during pregnancy in mice,” Biol. Reprod., 63, No. 1, 274–280 (2000).CrossRefPubMedGoogle Scholar
  17. 17.
    S. R. Ladyman and D. R. Grattan, “Suppression of leptin receptor messenger ribonucleic acid and leptin responsiveness in the ventromedial nucleus of the hypothalamus during pregnancy in the rat,” Endocrinology, 146, No. 9, 3868–3874 (2005).CrossRefPubMedGoogle Scholar
  18. 18.
    S. F. Leibowitz and K. E. Wortley, “Hypothalamic control of energy balance: different peptides, different functions,” Peptides, 25, No. 3, 473–504 (2004).CrossRefPubMedGoogle Scholar
  19. 19.
    T. M. Mizuno and C. V. Mobbs, “Hypothalamic agouti-related protein messenger ribonucleic acid is inhibited by leptin and stimulated by fasting,” Endocrinology, 140, No. 2, 814–817 (1999).CrossRefPubMedGoogle Scholar
  20. 20.
    A. Oberto, P. Mele, F. Zammaretti, G. Panzica, and C. Eva, “Evidence of altered neuropeptide Y content and neuropeptide Y1 receptor gene expression in the hypothalamus of pregnant transgenic mice,” Endocrinology, 144, No. 11, 4826–4830 (2003).CrossRefPubMedGoogle Scholar
  21. 21.
    M. Rocha, C. Bing, G. Williams, and M. Puerta, “Pregnancy-induced hyperphagia is associated with increased gene expression of hypothalamic agouti-related peptide in rats,” Regul. Pept., 114, No. 2–3, 159–165 (2003).CrossRefPubMedGoogle Scholar
  22. 22.
    R. M. Seeber, J. T. Smith, and B. J. Waddell, “Plasma leptin-binding activity and hypothalamic leptin receptor expression during pregnancy and lactation in the rat,” Biol. Reprod., 66, No. 6, 1762–1767 (2002).CrossRefPubMedGoogle Scholar
  23. 23.
    T. Shimokawa, M. V. Kumar, and M. D. Lane, “Effect of a fatty acid synthase inhibitor on food intake and expression of hypothalamic neuropeptides,” Proc. Natl. Acad. Sci. USA, 99, No. 1, 66–71 (2002).CrossRefPubMedGoogle Scholar
  24. 24.
    M. S. Smith and K. L. Grove, “Integration of the regulation of reproductive function and energy balance: lactation as a model,” Front. Neuroendocrinol., 23, No. 3, 225–256 (2002).CrossRefPubMedGoogle Scholar
  25. 25.
    R. G. Vernon, R. G. Denis, A. Sorensen, and G. Williams, “Leptin and the adaptations of lactation in rodents and ruminants,” Horm. Metab. Res., 34, No. 11–12, 678–685 (2002).CrossRefPubMedGoogle Scholar
  26. 26.
    Q. Wang, C. Bing, K. Al-Barazanji, D. E. Mossakowaska, X. M. Wang, D. L. McBay, W. A. Neville, M. Taddayon, L. Pickavance, S. Dryden, M. E. Thomas, M. T. McHale, I. S. Gloyer, S. Wilson, R. Buckingham, J. R. Arch, P. Trayhurn, and G. Williams, “Interactions between leptin and hypothalamic neuropeptide Y neurons in the control of food intake and energy homeostasis in the rat,” Diabetes, 46, No. 3, 335–341 (1997).CrossRefPubMedGoogle Scholar
  27. 27.
    J. P. Wilding, M. O. Ajala, P. D. Lambert, and S. R. Bloom, “Additive effects of lactation and food restriction to increase hypothalamic neuropeptide Y mRNA in rats,” J. Endocrinol., 152, No. 3, 365–369 (1997).CrossRefPubMedGoogle Scholar
  28. 28.
    S. C. Woods, R. J. Seeley, D. Porte, Jr., and M. W. Schwartz, “Signals that regulate food intake and energy homeostasis,” Science, 280, No. 5368, 1378–1383 (1998).CrossRefPubMedGoogle Scholar
  29. 29.
    M. Ziotopoulou, C. S. Mantzoros, S. M. Hileman, and J. S. Flier, “Differential expression of hypothalamic neuropeptides in the early phase of diet-induced obesity in mice,” Am. J. Physiol. Endocrinol., 279, No. 4, E838–E845 (2000).Google Scholar

Copyright information

© Springer Science+Business Media, Inc. 2010

Authors and Affiliations

  • E. N. Makarova
    • 1
  • E. D. Kochubei
    • 1
  • N. M. Bazhan
    • 1
  1. 1.Institute of Cytology and Genetics, Siberian BranchRussian Academy of SciencesNovosibirskRussia

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