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Neuroscience and Behavioral Physiology

, Volume 37, Issue 9, pp 921–928 | Cite as

Effects of protein synthesis inhibitors during reactivation of associative memory in the common snail induces reversible and irreversible amnesia

  • S. V. Solntseva
  • V. P. Nikitin
  • S. A. Kozyrev
  • A. V. Shevelkin
  • A. V. Lagutin
  • V. V. Sherstnev
Article

Abstract

The effects of protein synthesis inhibitors on the reactivation of an associative skill consisting of refusing a particular food by common snails were studied. Animals were given single injections of a protein synthesis inhibitor (cycloheximide at 0.6 mg/snail or anisomycin at 0.4 mg) 24 h after three days of training, and were then presented with a “reminding” stimulus (the “conditioned reflex” food-banana) and tested for retention of the skill. Observations revealed an impairment of reproduction of the acquired skill 2.5 h after the “reminder, ” with spontaneous restoration at 4.5–5.5 h. Other snails were given single 1.8-mg doses of cycloheximide or three 0.6-mg doses with intervals of 2 h. “Reminders” were presented after each injection. In these conditions, impairment of reproduction of the conditioned reflex also appeared 2.5 h after the first “reminder, ” though amnesia lasted at least 30 days and repeat training of the animals produced only partial recovery of the skill. Thus, we have provided the first demonstration that recovery of a long-term memory “trace” on exposure to relatively low doses of protein synthesis inhibitors produces transient and short-lived amnesia, lasting 2–3 h, while long-term, irreversible amnesia occurrs after longer-lasting or more profound suppression of protein synthesis. These results suggest that the “reminding” process induces reconsolidation of the “ initial” memory, suppression of which by protein synthesis inhibitors leads to “erasure” of the memory “trace” and impairs consolidation on repeat training.

Key words

associative memory reactivation of memory reconsolidation protein synthesis inhibition stages of memory common snail 

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References

  1. 1.
    T. Kh. Gainutdinova, R. R. Tagirova, A. I. Ismailova, L. N. Muranova, Kh. L. Gainutdinov, and P. M. Balaban, “Protein synthesis-dependent reactivation of a contextual conditioned reflex in the common snail,” Zh. Vyssh. Nerv. Deyat., 54, No. 6, 785–790 (2004).Google Scholar
  2. 2.
    K. V. Anokhin, A. A. Tiunova, and S. P. R. Rose, “Reminder effects-reconsolidation or retrieval deficit? Pharmacological dissection with protein synthesis inhibitors following reminder for a passive-avoidance task in young chicks,” Eur. J. Neurosci., 15, No. 11, 1759–1765 (2002).PubMedCrossRefGoogle Scholar
  3. 3.
    P. M. Balaban, “Declarative and procedural memory in animals with simple nervous systems,” in: Psychology at the Turn of the Millennium, C. Hofsten (ed.), Academic Press, Stockholm (2002), pp. 1–28.Google Scholar
  4. 4.
    B. Bozon, S. Davis, and S. Laroche, “A requirement for the immediate early gene zif268 in reconsolidation of recognition memory after retrieval,” Neuron, 40, No. 4, 695–701 (2003).PubMedCrossRefGoogle Scholar
  5. 5.
    F. M. Child, H. T. Epstein, A. M. Kuzirian, and D. L. Alkon, “Memory reconsolidation in Hermissenda,” Biol. Bull., 205, No. 2, 218–219 (2003).PubMedCrossRefGoogle Scholar
  6. 6.
    H. P. Davis and L. R. Squire, “Protein synthesis and memory: a review,” Psychol. Bull., 96, No. 4, 519–559 (1984).Google Scholar
  7. 7.
    J. Debiec, J. E. LeDoux, and K. Nader, “Cellular and systems reconsolidation in the hippocampus,” Neuron, 36, No. 3, 527–538 (2002).PubMedCrossRefGoogle Scholar
  8. 8.
    Y. Dudai and M. Eisenberg, “Rites of passage of the engram: reconsolidation and the lingering consolidation hypothesis,” Neuron, 44, No. 1, 93–100 (2004).PubMedCrossRefGoogle Scholar
  9. 9.
    S. Duvarci and K. Nader, “Characterization of fear memory reconsolidation,” J. Neurosci., 24, No. 42, 9269–9275 (2004).PubMedCrossRefGoogle Scholar
  10. 10.
    L. M. Igaz, M. R. M. Vianna, J. H. Medina, and I. Izquierdo, “Two time periods of hippocampal mRNA synthesis are required for memory consolidation of fear-motivated learning,” J. Neurosci., 22, No. 15, 6781–6789 (2002).PubMedGoogle Scholar
  11. 11.
    M. C. Inda, J. M. Delgado-Garcia, and A. M. Carrion, “Acquisition, consolidation, reconsolidation, and extinction of eyelid conditioning responses require de novo protein synthesis,” J. Neurosci., 25, No. 8, 2070–2080 (2005).PubMedCrossRefGoogle Scholar
  12. 12.
    K. M. Lattal and T. Abel, “Behavioral impairments caused by injections of the protein synthesis inhibitor anisomycin after contextual retrieval reverse with time,” Proc. Natl. Acad. Sci. USA, 101, No. 13, 4667–4672 (2004).PubMedCrossRefGoogle Scholar
  13. 13.
    P. G. Montarolo, P. Goelet, V. F. Castellucci, J. Morgan, E. R. Kandel, and S. Schasher, “A critical period for macromolecular synthesis in long-term heterosynaptic facilitation in Aplysia,” Science, 234, No. 4781, 1249–1254 (1986).PubMedCrossRefGoogle Scholar
  14. 14.
    K. Nader, “Memory traces unbound,” Trends Neurosci., 26, No. 2, 65–72 (2003).PubMedCrossRefGoogle Scholar
  15. 15.
    M. E. Pedreira, L. M. Perez-Cuesta, and H. Maldonado, “Reactivation and reconsolidation of long-term memory in the crab Chasmagnathus: protein synthesis requirement and mediation by NMDA-type glutamatergic receptors,” J. Neurosci., 22, No. 18, 8305–8311 (2002).PubMedGoogle Scholar
  16. 16.
    S. Sangha, A. Scheibenstock, and K. Lukowiak, “Reconsolidation of a long-term memory in Lymnaea requires new protein and RNA synthesis and the soma of right pedal dorsal 1,” J. Neurosci., 23, No. 22, 8034–8040 (2003).PubMedGoogle Scholar
  17. 17.
    S. J. Sara, “Retrieval and reconsolidation: toward a neurobiology of remembering,” Learn. Mem., 7, No. 2, 73–84 (2000).PubMedCrossRefGoogle Scholar
  18. 18.
    M. J. Summer, S. F. Crowe, and K. T. Ng, “Memory retrieval in the day-old chick: a psychobiological approach,” Neurosci. Biobehav. Rev., 27, 219–231 (2003).CrossRefGoogle Scholar
  19. 19.
    A. Suzuki, S. A. Josselyn, P. W. Frankland, S. Masushige, A. J. Silva, and S. Kida, “Memory reconsolidation and extinction have distinct temporal and biochemical signatures,” J. Neurosci., 24, No. 20, 4787–4795 (2004).PubMedCrossRefGoogle Scholar
  20. 20.
    A. A. Tiunova, K. V. Anokhin, and S. P. Rose, “Two critical periods of protein and glycoprotein synthesis in memory consolidation for visual categorization learning in chick,” Learn. Mem., 4, No. 4, 401–410 (1998).PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, Inc. 2007

Authors and Affiliations

  • S. V. Solntseva
    • 1
  • V. P. Nikitin
    • 1
  • S. A. Kozyrev
    • 1
  • A. V. Shevelkin
    • 1
  • A. V. Lagutin
    • 1
  • V. V. Sherstnev
    • 1
  1. 1.P. K. Anokhin Research Institute of Normal PhysiologyMoscowRussia

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