Neuroscience and Behavioral Physiology

, Volume 36, Issue 4, pp 409–413 | Cite as

Effects of glyprolines on stress-induced behavioral disorders in rats

  • S. E. Badmaeva
  • G. N. Kopylova
  • N. N. Abushinova
  • G. E. Samonina
  • B. A. Umarova


We report here studies on the antistress protective actions of three peptides of the glyproline family: Pro-Gly-Pro, Pro-Gly, and Gly-Pro. Stress (10 min forced swimming) evoked typical changes in the behavioral activity of rats in the elevated cross maze and hole board tests, providing evidence of a significant increase in anxiety and a decrease in the level of orientational-investigative activity. Prior (15 min before stress) i.p. administration of Pro-Gly-Pro and Gly-Pro at a dose of 3.7 μM/kg significantly decreased the stress-induced behavioral abnormalities. This demonstrates the possibility that peptides Pro-Gly-Pro and Gly-Pro may affect CNS structures involved in forming the body’s responses to stress-inducing factors. Peptide Pro-Gly, at an equimolar dose, had no marked protective effect and only slightly decreased the stress-induced abnormalities in the behavior of rats.

Key words

stress glyprolines behavior 


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  1. 1.
    I. P. Ashmarin, A. A. Kamenskii, L. A. Lyapina, N. F. Myasoedov, and G. E. Samonina, “Glyprolines as independent regulators and stabilizers of other peptides,” Vopr. Med. Biol. Farm. Khim., 1, 24–27 (2002).Google Scholar
  2. 2.
    I. P. Ashmarin, E. P. Karazeeva, L. A. Lyapina, and G. E. Samonina, “The regulatory activity of the very simple proline-containing peptides Pro-Gly, Gly-Pro, Pro-Gly-Pro, and Gly-Pro-Gly-Gly and possible sources for their biosynthesis,” Biokhimiya, 63, No. 2, 149–155 (1998).Google Scholar
  3. 3.
    Z. V. Bakaeva, K. E. Badmaeva, I. Yu. Sergeev, and G. E. Samonina, “The effects of glyprolines on the noradrenaline tone of isolated ring segments of rat aorta,” Byull. Éksperim. Biol. Med., 135, No. 4, 390–393 (2003).Google Scholar
  4. 4.
    B. V. Vas’kovskii, Yu. A. Zolotarev, S. E. Zhuikova, G. E. Samonina, I. P. Ashmarin, N. F. Myasoedov, L. A. Lyapina, V. E. Pastorova, and Z. V. Bakaeva, “Studies of the distribution of [3H]PRO-GLY-PRO in the rat,” Vopr. Med. Biol. Farm. Khim., 3, 45–47 (2003).Google Scholar
  5. 5.
    T. A. Gudasheva, S. S. Boiko, V. Kh. Akparov, R. U. Ostrovskaya, G. G. Rozantsev, T. A. Boronina, V. P. Zherdev, and S. B. Seredenin, “Identification of cycloprolylglycine, a new endogenous peptide with nootropic activity, in the rat brain,” Dokl. Ros. Akad. Nauk., 350, No. 6, 834–836 (1996).Google Scholar
  6. 6.
    T. A. Gudasheva, M. A. Konstantinopol’skii, R. U. Ostrovskaya, and S. B. Seredenina, “The anxiogenic activity of the endogenous nootropic dipeptide cycloprolylglycine in an elevated cross maze test,” Byull. Éksperim. Biol. Med., 131, No. 5, 547–550 (2001).Google Scholar
  7. 7.
    S. E. Zhuikova, K. E. Badmaeva, G. E. Samonina, and L. G. Plesskaya, “Semax and a number of glyproline peptides accelerate the healing of acetate ulcers in rats,” Éksp. Klin. Gastroénterol., 4, 88–92 (2003).PubMedGoogle Scholar
  8. 8.
    S. E. Zhuikova, Z. V. Bakaeva, and G. E. Samonina, “The differential antiulcer effects of possible metabolites of the peptide Pro-Gly-Pro — Pro-Gly and Gly-Pro — on standard and stress models of ulcers in rats,” Vestn. Mosk. Gos. Univ. Ser. 16 Biologiya, 2, 20–22 (2003).Google Scholar
  9. 9.
    S. E. Zhuikova, R. P. Khropycheva, Yu. A. Zolotarev, S. A. Polenov, and G. E. Samonina, “New peptide regulators of gastric secretion in rats,” Éksp. Klin. Gastroénterol., 2, 86–90 (2003).Google Scholar
  10. 10.
    Yu. A. Zolotarev, S. E. Zhuikova, I. P. Ashmarin, N. F. Myasoedov, B. V. Vas’kovskii, and G. E. Samonina, “Metabolism of the peptide Pro-Gly-Pro after different routes,” Byull. Éksperim. Biol. Med., 135, No. 4, 423–426 (2003).Google Scholar
  11. 11.
    M. M. Kozlovskaya, I. I. Kozlovskii, E. A. Val’dman, and S. B. Seredenin, “Selank and short peptides of the tuftsin family in the control of adaptive behavior in stress,” Zh. Vyssh. Nerv. Deyat., 88, No. 6, 751–761 (2002).Google Scholar
  12. 12.
    G. N. Kopylova, E. A. Smirnova, L. Ts. Sanzhieva, B. A. Umarova, T. V. Lelekova, and G. E. Samonina, “Glyprolines and Semax decrease stress-induced impairments of the peritoneal microcirculation,” Byull. Éksperim. Biol. Med., 136, No. 11, 497–499 (2003).Google Scholar
  13. 13.
    N. G. Levitskaya, N. V. Latysheva, L. A. Andreeva, and A. A. Kamenskii, “Behavioral effects of the tripeptide Pro-Gly-Pro,” Vestn. Mosk. Gos. Univ. Ser. 16 Biologiya, 2, 17–22 (2000).Google Scholar
  14. 14.
    V. E. Pastorova, L. A. Lyapina, T. Yu. Smolina, and I. P. Ashmarin, “The anticoagulant and fibrinolytic effects of short proline-containing peptides,” Izv. Ros. Akad. Nauk Ser. Biol., 3, 390–394 (1998).Google Scholar
  15. 15.
    G. E. Samonina, G. N. Kopylova, V. I. Sergeev, S. E. Zhuikova, and Z. V. Bakaeva, “Correction of gastric blood flow as a possible mechanism for the antiulcer effects of short proline-containing peptides,” Ros. Fiziol. Zh. im. I. M. Sechenova, 87, No. 11, 1488–1492 (2001).Google Scholar
  16. 16.
    S. B. Seredenin, T. A. Gudasheva, S. S. Boiko, G. I. Kovalev, M. V. Voronin, and M. A. Yarkova, “The endogenous dipeptide cycloprolylglycine has selective anxiolytic activity in animals with marked fear reactions,” Byull. Éksperim. Biol. Med., 133, No. 4, 417–419 (2002).Google Scholar
  17. 17.
    N. M. Timofeeva, N. N. Iezuitova, and L. V. Gromova, “Current concepts on the absorption of monosaccharides, amino acids, and peptides in the small intestine of mammals,” Usp. Fiziol. Nauk., 31, No. 4, 24–37 (2000).PubMedGoogle Scholar
  18. 18.
    B. A. Umarova, G. N. Kopylova, E. A. Smirnova, A. A. Guseva, and S. E. Zhuikova, “The secretory activity of mast cells in stress — the effects of the peptides prolyl-glycyl-proline and Semax,” Byull. Éksperim. Biol. Med., 136, No. 1, 371–373 (2003).Google Scholar
  19. 19.
    J. P. Bai and G. L. Amidon, “Structural specificity of mucosal-cell transport and metabolism of peptide drugs: implication for oral peptide drug delivery,” Pharm. Res., 9, No. 8, 969–978 (1992).CrossRefPubMedGoogle Scholar
  20. 20.
    G. E. Samonina, I. P. Ashmarin, and L. A. Lyapina, “Glyproline peptide family: review on bioactivity and possible origins,” Pathophysiol., 8, 229–234 (2002).Google Scholar
  21. 21.
    G. E. Samonina, L. A. Lyapina, G. N. Kopylova, V. E. Pastorova, Z. V. Bakaeva, N. Y. Jelliaznik, S. E. Zuikova, and I. P. Ashmarin, “Protection of gastric mucosal integrity by gelatin and simple proline-containing peptides,” Pathophysiol., 7, 69–73 (2000).Google Scholar

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • S. E. Badmaeva
    • 1
  • G. N. Kopylova
    • 1
  • N. N. Abushinova
    • 1
  • G. E. Samonina
    • 1
  • B. A. Umarova
    • 1
  1. 1.M. V. Lomonosov Moscow State University, Vorob’evy GoryMoscowRussia

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