Neuroscience and Behavioral Physiology

, Volume 35, Issue 2, pp 147–151 | Cite as

Aftereffects of microinjections of Neurotensin into the Substantia Nigra of the brain on conditioned motor responses in Rats with lesions to Serotoninergic neurons

  • N. P. Shugalev
  • G. Khartmann
  • E. Kertesh


The effects of neurotensin microinjections into the substantia nigra of the brain on the performance of motor reactions in response to positive (food reinforcement) and negative (unreinforced) conditioned signals were studied, along with the characteristic aftereffects of these microinjections in rats with lesions to serotoninergic neurons in the dorsal cervical nucleus. Lesions were produced by local administration of the selective neurotoxin 5,7-dihydroxytryptamine. Microinjections of neurotensin were found to weaken the disinhibitory effects of neurotoxin on extinction of intersignal responses and extinction of conditioned motor reactions in response to presentation of negative stimuli, but to have no marked effect on the performance of reactions evoked by positive conditioned signals. Changes persisted into subsequent experiments without peptide microinjections. Measurements of the level of preference of the animals for one quadrant of the experimental chamber showed that operated rats avoided occupying the test quadrant and preferred to locate themselves in the opposite quadrant. Neurotensin released this effect of the neurotoxin. These results lead to the conclusion that these behavioral effects of neurotensin are associated with its normalizing influences on the motivational-emotional state of the animals with lesions to serotoninergic neurons and may also be due to the formation in the animals of a contextual conditioned emotional state, which aids optimization of the adaptive functions of the brain.


neurotensin neurotoxin dopamine serotonin substantia nigra conditioned reflex motor activity aftereffects conditioned reflex preference 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Gromova, E. A. 1980Emotional Memory and Its MechanismsNaukaMoscow[in Russian]Google Scholar
  2. 2.
    Shugalev, N. P., Ol’shanskii, A. S., Khartmann, G. 2001Effects of neurotensin microinjections into the caudate nucleus of the rat brain on the performance and extinction of a conditioned motor reflexZh. Vyssh. Nerv. Deyat.51473476Google Scholar
  3. 3.
    Shugalev, N. P., Ol’shanskii, A. S., Khartmann, G. 2000Behavioral effects of neurotensin administered into the formations of the nigrostriatal system of the brainProceedings of the XXX Congress on Questions in Higher Nervous Activity15455[in Russian]Google Scholar
  4. 4.
    Cadet, J. L., Kajoko, K., Carlson, E., Epstein, C. J. 1993Autoradiographic distribution of 3[H] neurotensin receptors in the brain of superoxide dismutase transgenic miceSynapse142433Google Scholar
  5. 5.
    Dilts, R. P., Novitzki, M. R., Phan, T. H.,  et al. 1996Neurotensin inhibits the activation of midbrain serotoninergic neurons produced by random inescapable soundBrain Res.742294298Google Scholar
  6. 6.
    Ford, A. P., Marsden, C. A. 1990In vivo neurochemical and behavioral effects of intracerebrally administered neurotensin and D-Trp 11-neurotensin on mesolimbic and nigrostriatal dopaminergic function in the ratBrain Res.534243250Google Scholar
  7. 7.
    Fuxe, K., O’Connor, W., Antonelli, T. 1992Evidence for substrate of neuronal plasticity based on pre-and postsynaptic neurotensindopamine receptor interaction in the neostriatumProc. Natl. Acad. Sci. USA8955915594Google Scholar
  8. 8.
    Glimcher, P. W., Margolin, D. H., Giovano, A. A., Hoebel, B. J. 1984Neurotensin: A new reward peptideBrain Res.291119124Google Scholar
  9. 9.
    Hokfelt, R. E., Everitt, B. J., Thedorsson-Norhrim, E., Goldstein, M. 1984Occurrence of neurotensin-like immunoreactivity in subpopulations of hypothalamic, mesencephalic and medullary catecholamine neuronsJ. Comp. Neurol.222543559Google Scholar
  10. 10.
    Jolash, T., Aghajanian, G. K. 1997Neurotensin and the serotoninergic systemProgr. Neurobiol.52455458Google Scholar
  11. 11.
    Jolicoeur, F. B., Gagne, M. A., Rivest, R., Drumheller, A., St.-Pierre, S. 1993A typical neuroleptic-like behavioral effects of neurotensinBrain Res. Bull.32487491Google Scholar
  12. 12.
    Kalivas, P. W., Nemeroff, C. B., Prang, A. J. 1982Neuroanatomical site specific modulation of spontaneous motor activity by neurotensinEur. J. Pharmacol.78471474Google Scholar
  13. 13.
    Levant, B., Nemeroff, C. B. 1992Further studies on the modulation of regional brain neurotensin concentration by antipsychotic drugs: focus on haloperidol and BMY 14802J. Pharmacol. Exptl. Ther.262348355Google Scholar
  14. 14.
    Rompre, P. P. 1997Repeated activation of neurotensin receptors sensitizes to the stimulant effect of amphetamineEur. J. Pharmacol.328131134Google Scholar
  15. 15.
    Shi, W. X., Bunney, B. S. 1991Effects of neurotensin on midbrain dopamine neurones: are they mediated by formation of neurotensindopamine complex?Synapse9157164Google Scholar
  16. 16.
    Stove, Z. N., Nemeroff, C. B. 1991The electrophysiological action of neurotensin in the central nervous systemLife Sci.499871002Google Scholar
  17. 17.
    Werkman, T. R., Kruse, C. G., Nievelstein, H.,  et al. 2000Neurotensin attenuates the quinpirol-induced inhibition of the firing rate of dopamine neurons in the rat substantia nigra pars compacta and the ventral tegmental areaNeurosci.95417423Google Scholar

Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • N. P. Shugalev
    • 1
    • 2
  • G. Khartmann
    • 1
    • 2
  • E. Kertesh
    • 1
    • 2
  1. 1.Science Research Institute of the BrainRussian Academy of Medical SciencesMoscow
  2. 2.Neurophysiology Study Group, Hungarian Academy of Sciences at the Institute of PhysiologyPecs UniversityHungary

Personalised recommendations