This paper systematically studied the extraction process of total flavonoids from quinoa and the kinetics of macroporous resin purification. On the basis of single-factor experiments, the optimum extraction and purification process of total flavonoids from quinoa was studied by orthogonal experiments. The results showed that the optimum extraction conditions were as follows: ultrasonic time 25 min, temperature 200 °F, solid-liquid ratio 1:10 (g/mL), reflux time 2 h, and ethanol concentration 90%. Under this condition, the total flavonoids extracted from quinoa was 3.859 mg/g. Fifteen percent NaCl was added to salt out protein from flavonoid solution. The best macroporous resin purification process was as follows: pH 2, flow rate 2 BV/h, 0.3 mg/mL flavonoid solution loading, 8 BV water wash with acetone elution. Under this condition, the adsorption rate of purified flavonoids was 80.89%. The purity can be increased from 7.09% of the initial extract to 28.48%.
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This work was supported by the Key Laboratory of Coarse Cereal Processing, Ministry of Agriculture and Rural Affairs, Chengdu University (No. 2017Y0012).
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Xiaoyong, W., Yuan, T., Yanxia, S. et al. Extraction of flavonoids and kinetics of purification by macroporous resins from quinoa. J Nanopart Res 22, 181 (2020). https://doi.org/10.1007/s11051-020-04906-7
- Total flavonoids
- Macroporous resin