Peroxidase-like nanozyme sensing arrays for versatile analytes


Nanozyme biosensors are generally constructed by using the conventional lock-and-key strategy, which may not be available for some emerging analytes without specific recognition elements. In addition, it is difficult to carry out multiplex detection. To address these challenges, array-based sensing platforms have been constructed by using peroxidase-like nanozymes (i.e., “nanozyme sensor arrays”). In this review, we highlight the newly developed nanozyme sensor arrays and their applications for detection and discrimination of various analytes, including bioactive small molecules, proteins, and cancer cells. Moreover, we discuss the use of nanozyme sensor arrays for monitoring the enzymatic hydrolysis process by tracking patterns. They were successfully used for probing the hydrolytic processes of adenosine 5′-triphosphate disodium salt (ATP) and pyrophosphate (PPi) catalyzed by apyrase and PPase, respectively. To further enhance the robustness of output signals, ratiometric fluorescent sensor arrays were also constructed by using C3N4-based fluorescent nanozymes. Finally, some insights on future development for nanozyme sensor arrays were provided.

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  1. Cheng H, Liu Y, Hu Y, Ding Y, Lin S, Cao W, Wang Q, Wu J, Muhammad F, Zhao X, Zhao D, Li Z, Xing H, Wei H (2017) Monitoring of heparin activity in live rats using metal-organic framework nanosheets as peroxidase mimics. Anal Chem 89:11552–11559.

    CAS  Article  Google Scholar 

  2. Diehl KL, Anslyn EV (2013) Array sensing using optical methods for detection of chemical and biological hazards. Chem Soc Rev 42:8596–8611.

    CAS  Article  Google Scholar 

  3. Gao L, Zhuang J, Nie L, Zhang J, Zhang Y, Gu N, Wang T, Feng J, Yang D, Perrett S, Yan X (2007) Intrinsic peroxidase-like activity of ferromagnetic nanoparticles. Nat Nanotechnol 2:577–583.

    CAS  Article  Google Scholar 

  4. Geng Y, Peveler WJ, Rotello VM (2019) Array-based “chemical nose” sensing in diagnostics and drug discovery. Angew Chem Int Ed 58:5190–5200.

    CAS  Article  Google Scholar 

  5. Hatai J, Motiei L, Margulies D (2017) Analyzing amyloid beta aggregates with a combinatorial fluorescent molecular sensor. J Am Chem Soc 139:2136–2139.

    CAS  Article  Google Scholar 

  6. Hizir MS, Robertson NM, Balcioglu M, Alp E, Rana M, Yigit MV (2017) Universal sensor array for highly selective system identification using two-dimensional nanoparticles. Chem Sci 8:5735–5745.

    CAS  Article  Google Scholar 

  7. Huang W, Deng Y, He Y (2017) Visual colorimetric sensor array for discrimination of antioxidants in serum using MnO2 nanosheets triggered multicolor chromogenic system. Biosens Bioelectron 91:89–94.

    CAS  Article  Google Scholar 

  8. Huang Y, Ren J, Qu X (2019) Nanozymes: classification, catalytic mechanisms, activity regulation, and applications. Chem Rev 119:4357–4412.

    CAS  Article  Google Scholar 

  9. Li X, Kong C, Chen Z (2019) Colorimetric sensor arrays for antioxidant discrimination based on the inhibition of the oxidation reaction between 3,3 ',5,5 '-tetramethylbenzidine and hydrogen peroxides. ACS Appl Mater Interfaces 11:9504–9509.

    CAS  Article  Google Scholar 

  10. Li X, Wen F, Creran B, Jeong Y, Zhang X, Rotello VM (2012) Colorimetric protein sensing using catalytically amplified sensor arrays. Small 8:3589–3592.

    CAS  Article  Google Scholar 

  11. Pode Z, Peri-Naor R, Georgeson JM, Ilani T, Kiss V, Unger T, Markus B, Barr HM, Motiei L, Margulies D (2017) Protein recognition by a pattern-generating fluorescent molecular probe. Nat Nanotechnol 12:1161–1168.

    CAS  Article  Google Scholar 

  12. Qin L, Wang X, Liu Y, Wei H (2018) 2D-metal-organic-framework-nanozyme sensor arrays for probing phosphates and their enzymatic hydrolysis. Anal Chem 90:9983–9989.

    CAS  Article  Google Scholar 

  13. Qiu H, Pu F, Ran X, Liu C, Ren J, Qu X (2018) Nanozyme as artificial receptor with multiple readouts for pattern recognition. Anal Chem 90:11775–11779.

    CAS  Article  Google Scholar 

  14. Rana S, Elci SG, Mout R, Singla AK, Yazdani M, Bender M, Bajaj A, Saha K, Bunz UH, Jirik FR, Rotello VM (2016) Ratiometric array of conjugated polymers-fluorescent protein provides a robust mammalian cell sensor. J Am Chem Soc 138:4522–4529.

    CAS  Article  Google Scholar 

  15. Wang Q, Wei H, Zhang Z, Wang E, Dong S (2018a) Nanozyme: an emerging alternative to natural enzyme for biosensing and immunoassay. Trac-Trend Anal Chem 105:218–224.

  16. Wang X, Cao W, Qin L, Lin T, Chen W, Lin S, Yao J, Zhao X, Zhou M, Hang C, Wei H (2017) Boosting the peroxidase-like activity of nanostructured nickel by inducing its 3+ oxidation state in LaNiO3 perovskite and its application for biomedical assays. Theranostics 7:2277–2286.

    CAS  Article  Google Scholar 

  17. Wang X et al. (2019a) e g occupancy as an effective descriptor for the catalytic activity of perovskite oxide-based peroxidase mimics. Nat Commun 10:704.

  18. Wang X, Hu Y, Wei H (2016) Nanozymes in bionanotechnology: from sensing to therapeutics and beyond. Inorg Chem Front 3:41–60.

    CAS  Article  Google Scholar 

  19. Wang X, Qin L, Lin M, Xing H, Wei H (2019b) Fluorescent graphitic carbon nitride-based nanozymes with peroxidase-like activities for ratiometric biosensing. Anal Chem 91:10648–10656.

    CAS  Article  Google Scholar 

  20. Wang X, Qin L, Zhou M, Lou Z, Wei H (2018b) Nanozyme sensor arrays for detecting versatile analytes from small molecules to proteins and cells. Anal Chem 90:11696–11702.

    CAS  Article  Google Scholar 

  21. Wei X, Chen Z, Tan L, Lou T, Zhao Y (2017) DNA-catalytically active gold nanoparticle conjugates-based colorimetric multidimensional sensor array for protein discrimination. Anal Chem 89:556–559.

    CAS  Article  Google Scholar 

  22. Wu J, Wang X, Wang Q, Lou Z, Li S, Zhu Y, Qin L, Wei H (2019) Nanomaterials with enzyme-like characteristics (nanozymes): next-generation artificial enzymes (II). Chem Soc Rev 48:1004–1076.

    CAS  Article  Google Scholar 

  23. Zhang Z, Zhang X, Liu B, Liu J (2017) Molecular imprinting on inorganic nanozymes for hundred-fold enzyme specificity. J Am Chem Soc 139:5412–5419.

    CAS  Article  Google Scholar 

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This work was supported by the China Postdoctoral Science Foundation (2019TQ0144, and 2019M661786), National Natural Science Foundation of China (21722503, 21874067, and 91859112), 973 Program (2015CB659400), PAPD Program, Shuangchuang Program of Jiangsu Province, Open Funds of the State Key Laboratory of Analytical Chemistry for Life Science (SKLACLS1704), Open Funds of the State Key Laboratory of Coordination Chemistry (SKLCC1819), Open Funds of Key Laboratory of Analytical Chemistry for Biology and Medicine (Wuhan University), Ministry of Education (ACBM2019001), and Fundamental Research Funds for the Central Universities (021314380145).

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Wang, X., Wei, H. Peroxidase-like nanozyme sensing arrays for versatile analytes. J Nanopart Res 22, 22 (2020).

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  • Nanozymes
  • Sensor array
  • Peroxidase-like activity
  • Biosensing