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Short-term effects of ultrahigh concentration cationic silica nanoparticles on cell internalization, cytotoxicity, and cell integrity with human breast cancer cell line (MCF-7)

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Abstract

High concentrations of cationic colloidal silica nanoparticles (CCS-NPs) have been widely used for the enrichment of plasma membrane proteins. However, the interaction between the CCS-NPs and cells under the required concentration for the isolation of plasma membrane are rarely investigated. We evaluated the internalization and toxicity of the 15 nm CCS-NPs which were exposed at high concentrations with short time in human breast cancer cells (MCF-7) with transmission electron microscopy, energy dispersive X-ray spectroscopy, inductively coupled plasma atomic emission spectroscopy, and colorimetric assays. The NPs were observed throughout the cells, particularly in the cytoplasm and the nucleus, after short incubation periods. Additionally, the NPs significantly influenced the membrane integrity of the MCF-7 cells.

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Authors and Affiliations

  1. Graduate School of Nanoscience and Technology, Korea Advanced Institute of Science and Technology, Daejeon, Korea

    Ji Hyun Seog, Dongheun Kim & Sang Bok Lee

  2. Corning Precision Materials, Asan, Korea

    Bokyung Kong

  3. Department of Chemistry and Biochemistry, University of Maryland, College Park, MD, 20742, USA

    Lauren M. Graham & Sang Bok Lee

  4. Department of Biochemistry, Chungnam National University, Daejeon, Korea

    Joon Sig Choi

Authors
  1. Ji Hyun Seog
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  2. Bokyung Kong
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  3. Dongheun Kim
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  4. Lauren M. Graham
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  5. Joon Sig Choi
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  6. Sang Bok Lee
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Correspondence to Sang Bok Lee.

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Seog, J.H., Kong, B., Kim, D. et al. Short-term effects of ultrahigh concentration cationic silica nanoparticles on cell internalization, cytotoxicity, and cell integrity with human breast cancer cell line (MCF-7). J Nanopart Res 17, 6 (2015). https://doi.org/10.1007/s11051-014-2823-2

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  • DOI: https://doi.org/10.1007/s11051-014-2823-2

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