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In vitro cytotoxic effects of modified zinc oxide quantum dots on breast cancer cell lines (MCF7), colon cancer cell lines (HT29) and various fungi

  • Zahra Fakhroueian
  • Alireza Mozafari Dehshiri
  • Fatemeh Katouzian
  • Pegah Esmaeilzadeh
Research Paper

Abstract

An important ideal objective of this study was to perform surface functionalization of fine (1–3 nm) ZnO quantum dot nanoparticles (QD NPs) in order to inhibit decomposition and agglomeration of nanoparticles in aqueous media. Polymers, oily herbal fatty acids, PEG (polyethylene glycol), and organosilanes are the main reagents used in these reactions, because they are completely soluble in water, and can be used as biological probes in nanomedicine. Vegetable fatty acid-capped ZnO (QD NPs) was fabricated by dissolving at a suitable pH after sol–gel method in the presence of nonionic surfactants as efficient templates with a particular HLB (hydrophilic-lipophilic balance) value (9.7 and 8.2). In the present research, we focused on the cellular toxicity of fine zinc oxide QD NPs containing particular blue fluorescence for targeted delivery of MCF7 and HT29 cancer cell lines. The IC50 values were determined as 10.66 and 5.75 µg/ml for MCF7 and HT29, respectively. These findings showed that ZnO QDs have low toxicity in normal cells (MDBK) and can display potential application in cancer chemotherapy in the near future. These properties could result in the generation of a promising candidate in the field of nanobiomedicine. The robust-engineered ZnO QD NPs showed their antibacterial and antifungal activities against Bacillus anthracis, Staphylococcus aureus, Klebsiella pneumonia, and Staphylococcus epidermidis bacteria and also different fungi such as Microsporum gypseum, Microsporum canis, Trichophyton mentagrophytes, Candida albicans, and Candida tropicalis, compared with the standard antibiotic agents like Gentamicin and Clotrimazol.

Keywords

ZnO quantum dots Surface modification Breast cancer lines MCF7 Colon cancer cell lines HT29 Cytotoxic MDBK test Selective toxicity Antifungal drug Anticancer 

Notes

Acknowledgments

The authors wish to express our sincere and deep gratitude to Professor Dr. A. Shafiekhani in physics, Professor Dr. A. Vatani for incessant encouragements and spiritual supports. Also, authors would like to thank Mr. F. Harsini for great aids to our scientific research area.

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Zahra Fakhroueian
    • 1
  • Alireza Mozafari Dehshiri
    • 2
  • Fatemeh Katouzian
    • 3
  • Pegah Esmaeilzadeh
    • 4
  1. 1.School of Chemical Engineering, College of EngineeringUniversity of TehranTehranIran
  2. 2.Department of Traditional Pharmacy, Faculty of Traditional MedicineShahid Beheshti University of Medical SciencesTehranIran
  3. 3.Microbiology DepartmentAzad University of Pharmaceutical ScienceTehranIran
  4. 4.Biomedical Materials Group, Institute of PharmacyMartin Luther UniversityHalle-WittenbergGermany

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