Study of SiRNA-loaded PS-mPEG/CaP nanospheres on lung cancer
- 160 Downloads
An ultrasound-adsorption method was used to prepare Bcl-2-SiRNA-loaded PS-mPEG/CaP nanospheres. The size and zeta potential were 18.41 ± 4.31 nm (n = 5) and −23.5 ± 0.6 mV, respectively. The entrapment efficiency of SiRNA was 92.86 %. MTT assay results confirmed that the blank nanospheres demonstrated a negligible cytotoxicity response in H1299 cells. Flow cytometer analysis results demonstrated that PS-mPEG/CaP NSs could carry SiRNA into the cells effectively. RT-PCR experiments and apoptosis assay results approved that, compared with free SiRNA, SiRNA-loaded PS-mPEG/CaP NSs could silence Bcl-2 gene and induce cell apoptosis effectively. In vivo distribution results confirmed PS-mPEG/CaP NSs could carry SiRNA enter the tumor tissue effectively. Taken together, these results suggest that the Bcl-2-SiRNA-loaded PS-mPEG/CaP nanospheres have great potential to be used to cure lung cancer.
KeywordsUltrasonic-absorption method Bcl-2-SiRNA Gene silencing Targeting Lung cancer Nanomedicine
This study was supported by the National Science Foundation of People’s Republic of China (No. 81272568).
- Bradley G, Juranka PF, Ling V (1998) Mechanism of multidrug resistance. Biochim Biophys Acta 948:87–128Google Scholar
- Tsamandas AC, Kardamakis D, Tsiamalos P (2009) The potential role of Bcl-2 expression, apoptosis and cell proliferation (Ki-67 expression) in cases of gastric carcinoma and correlation with classic prognostic factors and patient outcome. Anticancer Res 29:703–709Google Scholar
- Wang Q, Liu PF, Sun Y, He ZL, Gong T, Sun X, Zhang ZR, Duan YR (2014c) Preparation and properties of biocompatible PS-PEG/calcium phosphate nanospheres. Nanotoxicology. doi: 10.3109/17435390.2014.911381