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Study of SiRNA-loaded PS-mPEG/CaP nanospheres on lung cancer

  • Qi Wang
  • Liubin Qin
  • Ying Sun
  • Ming Shen
  • Yourong Duan
Research Paper

Abstract

An ultrasound-adsorption method was used to prepare Bcl-2-SiRNA-loaded PS-mPEG/CaP nanospheres. The size and zeta potential were 18.41 ± 4.31 nm (n = 5) and −23.5 ± 0.6 mV, respectively. The entrapment efficiency of SiRNA was 92.86 %. MTT assay results confirmed that the blank nanospheres demonstrated a negligible cytotoxicity response in H1299 cells. Flow cytometer analysis results demonstrated that PS-mPEG/CaP NSs could carry SiRNA into the cells effectively. RT-PCR experiments and apoptosis assay results approved that, compared with free SiRNA, SiRNA-loaded PS-mPEG/CaP NSs could silence Bcl-2 gene and induce cell apoptosis effectively. In vivo distribution results confirmed PS-mPEG/CaP NSs could carry SiRNA enter the tumor tissue effectively. Taken together, these results suggest that the Bcl-2-SiRNA-loaded PS-mPEG/CaP nanospheres have great potential to be used to cure lung cancer.

Keywords

Ultrasonic-absorption method Bcl-2-SiRNA Gene silencing Targeting Lung cancer Nanomedicine 

Notes

Acknowledgments

This study was supported by the National Science Foundation of People’s Republic of China (No. 81272568).

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Qi Wang
    • 1
    • 2
  • Liubin Qin
    • 1
  • Ying Sun
    • 1
  • Ming Shen
    • 1
  • Yourong Duan
    • 1
  1. 1.State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, School of MedicineShanghai Jiao Tong UniversityShanghaiChina
  2. 2.Key Laboratory of Drug Targeting and Novel Drug Delivery Systems, Ministry of Education, West China School of PharmacySichuan UniversityChengduChina

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