Abstract
An ultrasound-adsorption method was used to prepare Bcl-2-SiRNA-loaded PS-mPEG/CaP nanospheres. The size and zeta potential were 18.41 ± 4.31 nm (n = 5) and −23.5 ± 0.6 mV, respectively. The entrapment efficiency of SiRNA was 92.86 %. MTT assay results confirmed that the blank nanospheres demonstrated a negligible cytotoxicity response in H1299 cells. Flow cytometer analysis results demonstrated that PS-mPEG/CaP NSs could carry SiRNA into the cells effectively. RT-PCR experiments and apoptosis assay results approved that, compared with free SiRNA, SiRNA-loaded PS-mPEG/CaP NSs could silence Bcl-2 gene and induce cell apoptosis effectively. In vivo distribution results confirmed PS-mPEG/CaP NSs could carry SiRNA enter the tumor tissue effectively. Taken together, these results suggest that the Bcl-2-SiRNA-loaded PS-mPEG/CaP nanospheres have great potential to be used to cure lung cancer.
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This study was supported by the National Science Foundation of People’s Republic of China (No. 81272568).
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Wang, Q., Qin, L., Sun, Y. et al. Study of SiRNA-loaded PS-mPEG/CaP nanospheres on lung cancer. J Nanopart Res 16, 2421 (2014). https://doi.org/10.1007/s11051-014-2421-3
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DOI: https://doi.org/10.1007/s11051-014-2421-3