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Targeted polymeric nanoparticles containing gold nanorods: a therapeutic approach against glioblastoma

  • Erica Locatelli
  • Wolfgang Bost
  • Marc Fournelle
  • Jordi Llop
  • Larraitz Gil
  • Francesca Arena
  • Vito Lorusso
  • Mauro Comes Franchini
Research Paper

Abstract

Chlorotoxin-targeted polymeric nanoparticles containing entrapped gold nanorods as potential therapeutic agent for glioblastoma multiforme have been developed and evaluated. In first proof of concept experiments, in vitro specific uptake in cancer cells and selective laser-induced cell death have been shown. In vivo studies with optical imaging showed increased retention of targeted NPs in the tumor.

Keywords

Nanorods Glioblastoma Brain tumor Chlorotoxin Polymeric micelles Laser irradiation Nanomedicine 

Supplementary material

11051_2014_2304_MOESM1_ESM.doc (4 mb)
Supplementary material 1 (DOC 4120 kb)

References

  1. Allen TM, Cullis PR (2004) Drug delivery systems: entering the mainstream. Science 303:1818–1822CrossRefGoogle Scholar
  2. Bonini BF, Camaggi CM, Comes Franchini M, Gentili D, Pession A, Rani M, Strocchi E (2010) Design and synthesis of novel 3,4-disubstituted pyrazoles for nanomedicine applications against malignant gliomas. Eur J Med Chem 45:2024–2033CrossRefGoogle Scholar
  3. Bost W, Lemor R, Fournelle M (2012) Comparison of the optoacoustic signal generation efficiency of different nanoparticular contrast agents. Appl Opt 51:8041–8046CrossRefGoogle Scholar
  4. Chen J, Wang D, Xi J, Au L, Siekkinen A, Warsen A, Li ZY, Zhang H, Xia Y, Li X (2007) Immuno gold nanocages with tailored optical properties for targeted photothermal destruction of cancer cells. Nano Lett 7:1318–1322CrossRefGoogle Scholar
  5. Chen J, Glaus C, Laforest R, Zhang Q, Yang M, Gidding M, Welch MJ, Xia Y (2010) Gold nanocages as photothermal transducers for cancer treatment. Small 6:811–817CrossRefGoogle Scholar
  6. Comes Franchini M, Ponti J, Lemor R, Fournelle M, Broggi F, Locatelli E (2010) Polymeric entrapped thiol-coated gold nanorods: cytotoxicity and suitability as molecular optoacoustic contrast agent. J Mater Chem 20:10908–10912CrossRefGoogle Scholar
  7. Duncan R (2003) The dawning era of polymer therapeutics. Nat Rev Drug Discov 2:347–360CrossRefGoogle Scholar
  8. Gazelle GS, Goldberg SN, Solbiati L, Livraghi T (2000) Tumor ablation with radio-frequency energy. Radiology 217:633–646CrossRefGoogle Scholar
  9. Gentili D, Ori G, Comes Franchini M (2009) Double phase transfer of gold nanorods for surface functionalization and entrapment into PEG-based nanocarriers. Chem Comm 45:5874–5876CrossRefGoogle Scholar
  10. Goldberg SN (2001) Radiofrequency tumor ablation: principles and techniques. Eur J Ultrasound 13:129–147CrossRefGoogle Scholar
  11. Hirsch LR, Stafford RJ, Bankson JA, Sershen SR, Rivera B, Price RE, Hazle JD, Halas NJ, West JL (2003) Nanoshell-mediated near-infrared thermal therapy of tumors under magnetic resonance guidance. Proc Natl Acad Sci USA 100:13549–13554CrossRefGoogle Scholar
  12. Hobbs SK, Monsky WL, Yuan F, Roberts WG, Griffith L, Torchilin VP, Jain RK (1998) Regulation of transport pathways in tumor vessels: role of tumor type and microenvironment. Proc Natl Acad Sci USA 95:4607–4612CrossRefGoogle Scholar
  13. Huang X, El-Sayed IH, Qian W, El-Sayed MA (2006) Cancer cell imaging and photothermal therapy in the near-infrared region by using gold nanorods. J Am Chem Soc 128:2115–2120CrossRefGoogle Scholar
  14. Huang Y, He S, Cao W, Cai K, Liang XJ (2012) Biomedical nanomaterials for imaging-guided cancer therapy. Nanoscale 4:6135–6149CrossRefGoogle Scholar
  15. Huber P, Debus J, Jenne J, Jochle K, van Kaick G, Lorenz WJ, Wannenmacher M (1996) Therapeutic ultrasound in tumor therapy. Principles, applications and new developments. Radiologe 36:64–71CrossRefGoogle Scholar
  16. Jana NR, Gearheart L, Murphy CJ (2001) Seed-mediated growth approach for shape-controlled synthesis of spheroidal and rod-like gold nanoparticles using a surfactant template. Adv Mater 13:1389–1393CrossRefGoogle Scholar
  17. Jemal A, Siegel R, Xu J, Ward E (2010) Cancer statistics. CA Cancer J Clin 60:277–300CrossRefGoogle Scholar
  18. Kremkau FW (1979) Cancer therapy with ultrasound: a historical review. J Clin Ultrasound 7:287–300CrossRefGoogle Scholar
  19. Levy-Nissenbaum E, Khan W, Pawar RP, Tabakman R, Naftali E, Winkler I, Kaufman O, Klapper L, Domba AJ (2012) Pharmacokinetic and efficacy study of cisplatin and paclitaxel formulated in a new injectable poly(sebacic-co-ricinoleic acid) polymer. Eur J Pharm Biopharm 82:85–93CrossRefGoogle Scholar
  20. Locatelli E, Comes Franchini M (2012) Biodegradable PLGA-b-PEG polymeric nanoparticles: synthesis, properties, and nanomedical applications as drug delivery system. J Nanopart Res 14:1316–1333CrossRefGoogle Scholar
  21. Locatelli E, Broggi F, Ponti J, Marmorato P, Lena S, Comes Franchini M (2012) Lipophilic silver nanoparticles and their polymeric entrapment into targeted-PEG-based micelles for the treatment of glioblastoma. Adv Healthcare Mater 1:342–347CrossRefGoogle Scholar
  22. Melancon MP, Lu W, Yang Z, Zhang R, Cheng Z, Elliot AM, Stafford J, Olson T, Zhang JZ, Li C (2008) In vitro and in vivo targeting of hollow gold nanoshells directed at epidermal growth factor receptor for photothermal ablation therapy. Mol Cancer Ther 7:1730–1739CrossRefGoogle Scholar
  23. Nikoobakht B, El-Sayed MA (2003) Preparation and growth mechanism of gold nanorods (NRs) using seed-mediated growth method. Chem Mater 15:1957–1962CrossRefGoogle Scholar
  24. Oyewumi MO, Yokel RA, Jay M, Coakley T, Mumper RJ (2004) Comparison of cell uptake, biodistribution and tumor retention of folate-coated and PEG-coated gadolinium nanoparticles in tumor-bearing mice. J Control Release 95:613–626CrossRefGoogle Scholar
  25. Sato M, Watanabe Y, Ueda S, Iseki S, Abe Y, Sato N, Kimura S, Okubo K, Onji M (1996) Microwave coagulation therapy for hepatocellular carcinoma. Gastroenterology 110:1507–1514CrossRefGoogle Scholar
  26. Seo HW, Kim DY, Kwon DY, Kwon JS, Jin LM, Lee B, Kim JH, Min BH, Kim MS (2013) Injectable intratumoral hydrogel as 5-fluorouracil drug depot. Biomaterials 34:2748–2757CrossRefGoogle Scholar
  27. Soroceanu L, Gillespie Y, Khazaeli MB, Sontheimer H (1998) Use of chlorotoxin for targeting of primary brain tumors. Cancer Res 58:4871–4879Google Scholar
  28. Veiseh O, Veiseh O, Sun C, Gunn J, Kohler N, Gabikian P, Lee D, Bhattarai N, Ellenbogen R, Sze R, Hallahan A, Olson J, Zhang M (2005) Optical and MRI multifunctional nanoprobe for targeting gliomas. Nano Lett 5:1003–1008CrossRefGoogle Scholar
  29. Veiseh O, Gunn JW, Kievit FM, Sun C, Fang CH, Lee JS, Zhang M (2009) Inhibition of tumor-cell invasion with chlorotoxin-bound superparamagnetic nanoparticles. Small 5:256–264CrossRefGoogle Scholar
  30. Wei J, Cheang T, Tang B, Xia H, Xing Z, Chen Z, Fang Y, Chen W, Xu A, Wang S, Luo J (2013) The inhibition of human bladder cancer growth by calcium carbonate/CaIP6 nanocomposite particles delivering AIB1 siRNA. Biomaterials 34:1246–1254CrossRefGoogle Scholar
  31. Xie J, Lee S, Chen X (2010) Nanoparticle-based theranostic agents. Adv Drug Deliv Rev 62:1064–1079CrossRefGoogle Scholar
  32. Yu MK, Park J, Jon S (2012) Targeting strategies for multifunctional nanoparticles in cancer imaging and therapy. Theranostics 2:3CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Erica Locatelli
    • 1
  • Wolfgang Bost
    • 2
  • Marc Fournelle
    • 2
  • Jordi Llop
    • 3
  • Larraitz Gil
    • 3
  • Francesca Arena
    • 4
  • Vito Lorusso
    • 5
  • Mauro Comes Franchini
    • 1
  1. 1.Department of Industrial Chemistry “Toso Montanari”University of BolognaBolognaItaly
  2. 2.Group Biomedical Ultrasound ResearchFraunhofer IBMTSankt IngbertGermany
  3. 3.Radiochemistry Department, Molecular Imaging UnitCIC BiomaGUNESan SebastiánSpain
  4. 4.Department of Molecular Biotechnology and Health Sciences-Molecular Biotechnology Center and Center of Preclinical ImagingUniversity of TorinoColleretto GiacosaItaly
  5. 5.Ephoran Multi Imaging SolutionsColleretto GiacosaItaly

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