Abstract
In this paper, we report a fabrication of streptavidin-coated magnetic nanoparticles used for DNA detection. Initially, amino-functionalized Fe3O4 nanoparticles with high saturation magnetization are prepared by a photopolymerization method using allylamine as monomer. It is followed by covalent immobilization of streptavidin onto the particle surface via a two-step reaction using glutaraldehyde as coupling agent. Streptavidin-coated magnetic nanoparticles are characterized and further tested for their ability to capture DNA target after binding biotinylated oligonucleotide probes. The results show that the products (~27.2 nm) have a maximum biotin-binding capacity of 0.71 nmol mg−1 when the immobilization reaction is conducted with a mass ratio of streptavidin to magnetic carriers above 0.2 in phosphate buffered saline (pH 7.4) for 24 h. In addition, highly negative ζ-potential and good magnetic susceptibility of the nanocomposites make them applicable for DNA collection and detection, which is verified by the results from the preliminary application of streptavidin-coated magnetic nanoparticles in DNA detection. Therefore, the magnetic nanoparticles provide a promising approach for rapid collection and detection of gene.
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Acknowledgments
This study was sponsored by the National Natural Science Foundation of China (Grant No. 21201151), Natural Scientific Foundation of Zhejiang Province (No. Y4100074, Y4090636, and Y4110025), Sci-Tech Research Projects of Jinhua (No. 2010-1-120 and 2010-1-069), and SRF for ROCS, SEM.
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Gong, P., Peng, Z., Wang, Y. et al. Synthesis of streptavidin-conjugated magnetic nanoparticles for DNA detection. J Nanopart Res 15, 1558 (2013). https://doi.org/10.1007/s11051-013-1558-9
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DOI: https://doi.org/10.1007/s11051-013-1558-9