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Studies on interaction and illumination damage of CS-Fe3O4@ZnS:Mn to bovine serum albumin

  • Li Liu
  • Ling Xiao
  • Hua-Yue Zhu
  • Xiao-Wen Shi
Research Paper

Abstract

In this study, the interaction of chitosan (CS) coated CS-Fe3O4@ZnS:Mn magnetic-fluorescent nanoparticles (MFNPs) with bovine serum albumin (BSA) was studied by means of ultraviolet–visible and fluorescence spectra; evidences for the damage of BSA molecule in the presence of CS-Fe3O4@ZnS:Mn MFNPs under UV illumination were also obtained. The results show that the dominating fluorescence quenching mechanism of CS-Fe3O4@ZnS:Mn MFNPs with BSA belongs to static quenching. Fluorescence resonance energy transfer (FRET) occurred from BSA to CS-Fe3O4@ZnS:Mn MFNPs. The thermodynamic parameters indicated that electrostatic interaction play major roles in CS-Fe3O4@ZnS:Mn-BSA, while binding processes exist spontaneously. In addition, the damage of CS-Fe3O4@ZnS:Mn MFNPs to BSA molecule under UV illumination was studied under various experimental parameters. It was proved that: the damage of BSA is prone to happen in the presence of CS-Fe3O4@ZnS:Mn MFNPs under UV illumination, there is synergic effect of oxygen and UV illumination on the damage of BSA, and the fluorescence quenching of BSA by CS-Fe3O4@ZnS:Mn MFNPs under UV illumination is mainly a result of a photo-induced free radical procedure.

Keywords

Magnetic-fluorescent nanoparticles Bovine serum albumin (BSA) Interaction UV illumination Reactive oxygen species (ROS) 

Notes

Acknowledgments

This work is supported by Development Program of China (863 program, grant number 2007AA06Z418) and the Fundamental Research Funds for the Central Universities.

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Li Liu
    • 1
    • 2
  • Ling Xiao
    • 1
    • 2
  • Hua-Yue Zhu
    • 1
    • 2
  • Xiao-Wen Shi
    • 1
    • 2
  1. 1.College of Resource and Environmental Science, Hubei Biomass-Resource Chemistry and Environmental Biotechnology Key LaboratoryWuhan UniversityWuhanPeople’s Republic of China
  2. 2.The Luojia Mountain of WuchangWuhanPeople’s Republic of China

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