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Studies on interaction and illumination damage of CS-Fe3O4@ZnS:Mn to bovine serum albumin

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Abstract

In this study, the interaction of chitosan (CS) coated CS-Fe3O4@ZnS:Mn magnetic-fluorescent nanoparticles (MFNPs) with bovine serum albumin (BSA) was studied by means of ultraviolet–visible and fluorescence spectra; evidences for the damage of BSA molecule in the presence of CS-Fe3O4@ZnS:Mn MFNPs under UV illumination were also obtained. The results show that the dominating fluorescence quenching mechanism of CS-Fe3O4@ZnS:Mn MFNPs with BSA belongs to static quenching. Fluorescence resonance energy transfer (FRET) occurred from BSA to CS-Fe3O4@ZnS:Mn MFNPs. The thermodynamic parameters indicated that electrostatic interaction play major roles in CS-Fe3O4@ZnS:Mn-BSA, while binding processes exist spontaneously. In addition, the damage of CS-Fe3O4@ZnS:Mn MFNPs to BSA molecule under UV illumination was studied under various experimental parameters. It was proved that: the damage of BSA is prone to happen in the presence of CS-Fe3O4@ZnS:Mn MFNPs under UV illumination, there is synergic effect of oxygen and UV illumination on the damage of BSA, and the fluorescence quenching of BSA by CS-Fe3O4@ZnS:Mn MFNPs under UV illumination is mainly a result of a photo-induced free radical procedure.

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Acknowledgments

This work is supported by Development Program of China (863 program, grant number 2007AA06Z418) and the Fundamental Research Funds for the Central Universities.

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Correspondence to Ling Xiao.

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Liu, L., Xiao, L., Zhu, HY. et al. Studies on interaction and illumination damage of CS-Fe3O4@ZnS:Mn to bovine serum albumin. J Nanopart Res 15, 1394 (2013). https://doi.org/10.1007/s11051-012-1394-3

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  • DOI: https://doi.org/10.1007/s11051-012-1394-3

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