Organometallic approach to polymer-protected antibacterial silver nanoparticles: optimal nanoparticle size-selection for bacteria interaction

  • Julian Crespo
  • Jorge García-Barrasa
  • José M. López-de-Luzuriaga
  • Miguel Monge
  • M. Elena Olmos
  • Yolanda Sáenz
  • Carmen Torres
Research Paper


The optimal size-specific affinity of silver nanoparticles (Ag NPs) towards E. coli bacteria has been studied. For this purpose, Ag NPs coated with polyvinylpyrrolidone (PVP) and cellulose acetate (CA) have been prepared using an organometallic approach. The complex NBu4[Ag(C6F5)2] has been treated with AgClO4 in a 1:1 molar ratio giving rise to the nanoparticle precursor [Ag(C6F5)] in solution. Addition of an excess of PVP (1) or CA (2) and 5 h of reflux in tetrahydrofuran (THF) at 66 °C leads to Ag NPs of small size (4.8 ± 3.0 nm for PVP-Ag NPs and 3.0 ± 1.2 nm for CA-Ag NPs) that coexist in both cases with larger nanoparticles between 7 and 25 nm. Both nanomaterials display a high antibacterial effectiveness against E. coli. The TEM analysis of the nanoparticle–bacterial cell membrane interaction shows an optimal size-specific affinity for PVP-Ag NPs of 5.4 ± 0.7 nm in the presence of larger size silver nanoparticles.

Graphical Abstract

An organometallic approach permits the synthesis of small size silver nanoparticles (ca 5 nm) as a main population in the presence of larger size nanoparticles. Optimal silver nanoparticle size-selection (5.4 nm) for the interaction with the bacterial membrane is achieved.


Silver nanoparticles Polymers Antibacterial TEM 



The D.G.I. (MEC)/FEDER (CTQ2010-20500-C02-02) and European Commission, POCTEFA (METNANO, EFA 17/08) projects are acknowledged for financial support. J. Crespo thanks the Comunidad Autónoma de La Rioja (CAR) for a grant. We thank the Servicio de Microscopía Electrónica de Materiales (Servicio General de Apoyo a la Investigación), Universidad de Zaragoza and at the Universidad de Cantabria (SERMET) for microscopy facilities.

Supplementary material

11051_2012_1281_MOESM1_ESM.pdf (6.9 mb)
Electronic Supplementary Information Size histograms for PVP-Ag NPs (1) alone and in the presence of E. coli; additional TEM micrographs of PVP-Ag NPs (1) alone and in the presence of bacteria. (PDF 7075 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Julian Crespo
    • 1
  • Jorge García-Barrasa
    • 1
  • José M. López-de-Luzuriaga
    • 1
  • Miguel Monge
    • 1
  • M. Elena Olmos
    • 1
  • Yolanda Sáenz
    • 2
  • Carmen Torres
    • 2
    • 3
  1. 1.Departamento de QuímicaUniversidad de La Rioja, Centro de Investigación en Síntesis Química (CISQ)LogroñoSpain
  2. 2.Área de Microbiología MolecularCentro de Investigación Biomédica de La RiojaLogroñoSpain
  3. 3.Departamento de Agricultura y Alimentación, Área de Bioquímica y Biología MolecularUniversidad de La RiojaLogroñoSpain

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