Mesoporous silica-coated superparamagnetic particles prepared by pseudomorphic transformation and their application in purification of plasmid DNA
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Mesoporous silica-coated superparamagnetic particles were prepared via pseudomorphic transformation of pre-made amorphous silica-coated Fe3O4–polymer composite particles using n-cetyltrimethylammonium bromide (CTAB) and 1,3,5-trimethyl benzene (MES) as template. The mesoporous particles presented almost the same size, shape, and magnetic property as the original amorphous particles but an ordered mesoporous shell with wormhole-like pore structure. The pore size of the shells increased from 2.4 to 3.1 and 4.2 nm as the molar ratio of MES/CTAB increased from 0 to 1.0 and 1.5. DNA extraction experiments showed the mesoporous particles were qualified for purification of plasmid DNA from bacterial lysate.
KeywordsSilica Mesoporous Superparamagnetic Pseudomorphic transformation DNA Nanomedicine
This study was supported by National Basic Research Program of China (No. 2011CB935800) and the National Nature Science Foundation of China (No. 51072064, 50825202).
- Kim J, Lee JE, Lee J, Yu JH, Kim BC, An K, Hwang Y, Shin CH, Park JG, Kim J, Hyeon T (2006) Magnetic fluorescent delivery vehicle using uniform mesoporous silica spheres embedded with monodisperse magnetic and semiconductor nanocrystals. J Am Chem Soc 128:688–689. doi: 10.1021/ja0565875 CrossRefGoogle Scholar
- Kim J, Kim HS, Lee N, Kim T, Kim H, Yu T, Song IC, Moon W, Hyeon T (2008) Multifunctional uniform nanoparticles composed of a magnetite nanocrystal core and a mesoporous silica shell for magnetic resonance and fluorescence imaging and for drug delivery. Angew Chem Int Ed 47:8438–8441. doi: 10.1002/anie.200802469 CrossRefGoogle Scholar
- Lee JE, Lee N, Kim H, Kim J, Choi SH, Kim JH, Kim T, Song IC, Park SP, Moon WK, Hyeon T (2010) Uniform mesoporous dye-doped silica nanoparticles decorated with multiple magnetite nanocrystals for simultaneous enhanced magnetic resonance imaging, fluorescence imaging, and drug delivery. J Am Chem Soc 132:552–557. doi: 10.1021/ja905793q CrossRefGoogle Scholar
- Martin T, Galarneau A, Di Renzo F, Fajula F, Plee D (2002) Morphological control of MCM-41 by pseudomorphic synthesis. Angew Chem Int Ed 41:2590–2592. doi: 10.1002/1521-3773(20020715)41:14<2590:AID-ANIE2590>3.0.CO;2-3 CrossRefGoogle Scholar
- Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, New YorkGoogle Scholar