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Cerium oxide and platinum nanoparticles protect cells from oxidant-mediated apoptosis

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Abstract

Catalytic nanoparticles represent a potential clinical approach to replace or correct aberrant enzymatic activities in patients. Several diseases, including many blinding eye diseases, are promoted by excessive oxidant stress due to reactive oxygen species (ROS). Cerium oxide and platinum nanoparticles represent two potentially therapeutic nanoparticles that de-toxify ROS. In the present study, we directly compare these two classes of catalytic nanoparticles. Cerium oxide and platinum nanoparticles were found to be 16 ± 2.4 and 1.9 ± 0.2 nm in diameter, respectively. Using surface plasmon-enhanced microscopy, we find that these nanoparticles associate with cells. Furthermore, cerium oxide and platinum nanoparticles demonstrated superoxide dismutase catalytic activity, but did not promote hemolytic or cytolytic pathways in living cells. Importantly, both cerium oxide and platinum nanoparticles reduce oxidant-mediated apoptosis in target cells as judged by the activation of caspase 3. The ability to diminish apoptosis may contribute to maintaining healthy tissues.

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Acknowledgment

This work was supported by NIH grant EY 019986 to H.R.P.

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Authors and Affiliations

  1. Department of Ophthalmology and Visual Sciences, The University of Michigan Medical School, 1000 Wall Street, Ann Arbor, MI, 48105, USA

    Andrea Clark, Aiping Zhu & Howard R. Petty

  2. Department of Materials Science and Engineering, The University of Michigan, Ann Arbor, MI, 48109, USA

    Kai Sun

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  1. Andrea Clark
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  2. Aiping Zhu
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  3. Kai Sun
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  4. Howard R. Petty
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Correspondence to Howard R. Petty.

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Clark, A., Zhu, A., Sun, K. et al. Cerium oxide and platinum nanoparticles protect cells from oxidant-mediated apoptosis. J Nanopart Res 13, 5547–5555 (2011). https://doi.org/10.1007/s11051-011-0544-3

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  • DOI: https://doi.org/10.1007/s11051-011-0544-3

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