Cerium oxide and platinum nanoparticles protect cells from oxidant-mediated apoptosis
Catalytic nanoparticles represent a potential clinical approach to replace or correct aberrant enzymatic activities in patients. Several diseases, including many blinding eye diseases, are promoted by excessive oxidant stress due to reactive oxygen species (ROS). Cerium oxide and platinum nanoparticles represent two potentially therapeutic nanoparticles that de-toxify ROS. In the present study, we directly compare these two classes of catalytic nanoparticles. Cerium oxide and platinum nanoparticles were found to be 16 ± 2.4 and 1.9 ± 0.2 nm in diameter, respectively. Using surface plasmon-enhanced microscopy, we find that these nanoparticles associate with cells. Furthermore, cerium oxide and platinum nanoparticles demonstrated superoxide dismutase catalytic activity, but did not promote hemolytic or cytolytic pathways in living cells. Importantly, both cerium oxide and platinum nanoparticles reduce oxidant-mediated apoptosis in target cells as judged by the activation of caspase 3. The ability to diminish apoptosis may contribute to maintaining healthy tissues.
KeywordsCatalytic nanoparticles Reactive oxygen metabolites Cell toxicity Apoptosis Nanomedicine
This work was supported by NIH grant EY 019986 to H.R.P.
- Asati A, Santra S, Kaittanis C, Nath S, Perez JM (2009) Oxidase-like activity of polymer-coated cerium oxide nanoparticles. Angew Chem Int Ed Engl 48:2308–2312Google Scholar
- Chen Q, Lu T, Xu M, Meng C, Hu Y, Sun K, Shlimak I (2011) Fabrication of uniform Ge-nanocrystals embedded in amorphous SiO2 films using Ge-ion implantation and neutron irradiation methods. Appl Phys Lett 98:073103-1–073103-3Google Scholar
- Elswaifi SF, Palmieri JR, Hockey KS, Rzigalinski BA (2009) Antioxidant nanoparticles for control of infectious disease. Infect Disord Drug Targets 9:445–452Google Scholar
- Kim Y-J, Kim D, Lee Y, Choi SY, Park J, Lee SY, Park JW, Kwon HJ (2009) Effects of nanoparticles saponin-platinum conjugates on 2,4-dinitrofluorobenzene-induced macrophage inflammatory protein-2 gene expression via oxygen species production in RAW 264.7 cells. BMB Rep 42:304–309CrossRefGoogle Scholar
- Osborn NM (2008) Involvement of oxidative stress in the pathogenesis of glaucoma. In: Cadenas E, Zierhut M, Rao NA (eds) Free radicals in ophthalmic disorders. Informa Healthcare, New York, NYGoogle Scholar
- Wu GS, Zhang J, Rao NA (1997) Peroxynitrite and oxidative damage in experimental autoimmune uveitis. Invest Ophthal Vis Sci 38:1333–1339Google Scholar