Journal of Nanoparticle Research

, Volume 11, Issue 2, pp 279–288 | Cite as

Extracellular biosynthesis of monodispersed gold nanoparticles by a SAM capping route

  • Li Wen
  • Zhonghua Lin
  • Pingying Gu
  • Jianzhang Zhou
  • Bingxing Yao
  • Guoliang Chen
  • Jinkun Fu
Research Paper


Monodispersed gold nanoparticles capped with a self-assembled monolayer of dodecanethiol were biosynthesized extracellularly by an efficient, simple, and environmental friendly procedure, which involved the use of Bacillus megatherium D01 as the reducing agent and the use of dodecanethiol as the capping ligand at 26 °C. The kinetics of gold nanoparticle formation was followed by transmission electron microscope (TEM) and UV-vis spectroscopy. It was shown that reaction time was an important parameter in controlling the morphology of gold nanoparticles. The effect of thiol on the shape, size, and dispersity of gold nanoparticles was also studied. The results showed that the presence of thiol during the biosynthesis could induce the formation of small size gold nanoparticles (<2.5 nm), hold the shape of spherical nanoparticles, and promote the monodispersity of nanoparticles. Through the modulation of reaction time and the use of thiol, monodispersed spherical gold nanoparticles capped with thiol of 1.9 ± 0.8 nm size were formed by using Bacillus megatherium D01.


Gold nanoparticles Extracellular biosynthesis Monodispersity Control of particle size Self-assembled monolayer Nanobiotechnology 



The project was supported by the National Natural Science Foundation of China (20433040 and 20423002) and the State Key Laboratory for Physical Chemistry of the Solid Surface, Xiamen University of China (200408).


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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Li Wen
    • 1
  • Zhonghua Lin
    • 2
  • Pingying Gu
    • 1
  • Jianzhang Zhou
    • 2
  • Bingxing Yao
    • 3
  • Guoliang Chen
    • 1
  • Jinkun Fu
    • 1
  1. 1.Department of Chemistry, College of Chemistry and Chemical EngineeringXiamen UniversityXiamenChina
  2. 2.State Key Laboratory of Physical Chemistry of Solid SurfacesXiamen UniversityXiamenChina
  3. 3.School of Life SciencesXiamen UniversityXiamenChina

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