Journal of Nanoparticle Research

, Volume 10, Issue 1, pp 151–162 | Cite as

Hydrophobically modified chitosan/gold nanoparticles for DNA delivery

  • Shanta Raj Bhattarai
  • Remant Bahadur K.C.
  • Santosh Aryal
  • Narayan Bhattarai
  • Sun Young Kim
  • Ho Keun Yi
  • Pyoung Han Hwang
  • Hak Yong Kim
Research Paper


Present study dealt an application of modified chitosan gold nanoparticles (Nac-6-Au) for the immobilization of necked plasmid DNA. Gold nanoparticles stabilized with N-acylated chitosan were prepared by graft-onto approach. The stabilized gold nanoparticles were characterized by different physico-chemical techniques such as UV-vis, TEM, ELS and DLS. MTT assay was used for in vitro cytotoxicity of the nanoparticles into three different cell lines (NIH 3T3, CT-26 and MCF-7). The formulation of plasmid DNA with the nanoparticles corresponds to the complex forming capacity and in-vitro/in-vivo transfection efficiency was studied via gel electrophoresis and transfection methods, respectively. Results showed the modified chitosan gold nanoparticles were well-dispersed and spherical in shape with average size around 10∼12 nm in triple distilled water at pH 7.4, and showed relatively no cytotoxicity at low concentration. Addition of plasmid DNA on the aqueous solution of the nanoparticles markedly reduced surface potential (50.0∼66.6%) as well as resulted in a 13.33% increase in hydrodynamic diameters of the formulated nanoparticles. Transfection efficiency of Nac-6-Au/DNA was dependent on cell type, and higher β-galactosidase activity was observed on MCF-7 breast cancer cell. Typically, this activity was 5 times higher in 4.5 mg/ml nanoparticles concentration than that achieved by the nanoparticles of other concentrations (and/or control). However, this activity was lower in in-vitro and dramatically higher in in-vivo than that of commercially available transfection kit (Lipofectin®) and DNA. From these results, it can be expected to develop alternative new vectors for gene delivery.


Chitosan DNA delivery Gene therapy Gold nanoparticles Non viral vectors Nanomedicine 



This work was supported by the Regional Research Centers Program of the Korean Ministry of Educational and Human Resources Development through the center for Healthcare Technology Development.


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Copyright information

© Springer Science+Business Media, Inc. 2007

Authors and Affiliations

  • Shanta Raj Bhattarai
    • 1
  • Remant Bahadur K.C.
    • 1
  • Santosh Aryal
    • 1
  • Narayan Bhattarai
    • 2
  • Sun Young Kim
    • 3
  • Ho Keun Yi
    • 4
  • Pyoung Han Hwang
    • 3
  • Hak Yong Kim
    • 5
  1. 1.Department of Bionanosystem EngineeringChonbuk National UniversityChonjuRepublic of Korea
  2. 2.Department of Materials Science and EngineeringUniversity of WashingtonSeattleUSA
  3. 3.Department of Pediatrics, School of MedicineChonbuk National UniversityChonjuRepublic of Korea
  4. 4.Department of Biochemistry, School of DentistyChonbuk National UniversityChonjuRepublic of Korea
  5. 5.Department of Textile EngineeringChonbuk National UniversityChonjuRepublic of Korea

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