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Gene Disruption in Scedosporium aurantiacum: Proof of Concept with the Disruption of SODC Gene Encoding a Cytosolic Cu,Zn-Superoxide Dismutase

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Abstract

Scedosporium species are opportunistic pathogens responsible for a large variety of infections in humans. An increasing occurrence was observed in patients with underlying conditions such as immunosuppression or cystic fibrosis. Indeed, the genus Scedosporium ranks the second among the filamentous fungi colonizing the respiratory tracts of the CF patients. To date, there is very scarce information on the pathogenic mechanisms, at least in part because of the limited genetic tools available. In the present study, we successfully developed an efficient transformation and targeted gene disruption approach on the species Scedosporium aurantiacum. The disruption cassette was constructed using double-joint PCR procedure, and resistance to hygromycin B as the selection marker. This proof of concept was performed on the functional gene SODC encoding the Cu,Zn-superoxide dismutase. Disruption of the SODC gene improved susceptibility of the fungus to oxidative stress. This technical advance should open new research areas and help to better understand the biology of Scedosporium species.

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Acknowledgements

TEM experiments were performed at SCIAM (Service Commun d’Imagerie et d’Analyses Microscopiques) platform, in Angers University.

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Authors and Affiliations

  1. Groupe d’Etude des Interactions Hôte-Pathogène, EA 3142, UNIV Angers, UNIV Brest, Université Bretagne-Loire, Angers, France

    Victoire Pateau, Bienvenue Razafimandimby, Jean-Philippe Bouchara & Sandrine Giraud

  2. Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Angers, France

    Patrick Vandeputte & Jean-Philippe Bouchara

  3. Biosciences, College of Life and Environmental Sciences, University of Exeter, Stocker Road, Exeter, EX4 4QD, UK

    Christopher R. Thornton

  4. IRHS, INRA, AGROCAMPUS-Ouest, Université Bretagne-Loire, Angers, SFR 4207 QUASAV, 42 rue Georges Morel, 49071, Beaucouzé Cedex, France

    Thomas Guillemette

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  1. Victoire Pateau
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Correspondence to Sandrine Giraud.

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Pateau, V., Razafimandimby, B., Vandeputte, P. et al. Gene Disruption in Scedosporium aurantiacum: Proof of Concept with the Disruption of SODC Gene Encoding a Cytosolic Cu,Zn-Superoxide Dismutase. Mycopathologia 183, 241–249 (2018). https://doi.org/10.1007/s11046-017-0204-y

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  • DOI: https://doi.org/10.1007/s11046-017-0204-y

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