, Volume 182, Issue 3–4, pp 339–347 | Cite as

Genotyping and Persistence of Candida albicans from Pregnant Women with Vulvovaginal Candidiasis

  • Cecilia V. Tapia
  • Germán Hermosilla
  • Paula Fortes
  • Claudio Alburquenque
  • Sergio Bucarey
  • Hugo Salinas
  • Paula I. Rodas
  • María Cristina Díaz
  • Fabien Magne



To study Candida albicans genotypes using RAPD and their susceptibility to fluconazole in healthy pregnant women and in vulvovaginal candidiasis (VVC) patients after topical treatment with clotrimazole.


Vaginal swabs were collected at t = 0 and t = 1 (1 month later) in pregnant women (control group, n = 33), and before (t = 0), at 1 month (t = 1) and at 2 months (t = 2) after clotrimazole treatment in pregnant women with VVC.


Candida albicans was isolated in 30% of healthy pregnant women and 80% of patients with VVC. A high genetic heterogeneity was observed in C. albicans genotypes between individuals. In patients with VVC, topical antifungal treatment with clotrimazole was clinically effective, but only in a 62% C. albicans was eradicated. In patients in which C. albicans was not eradicated, this microorganism persisted for 1 or 2 months after the antifungal treatment. The persistent colonies were not associated with a specific genotype, but they were associated with higher MICs in comparison with colonies isolated from the control group.


Therapy with topical clotrimazole, despite a good clinical outcome, could not eradicate completely C. albicans allowing the persistence of genotypes, with higher MICs to fluconazole. More studies with higher number of patients are needed to validate this preliminary finding.


Genotypes Persistence RAPD Resistance C. albicans Vulvovaginal candidiasis Clotrimazole Pregnant women 


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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  1. 1.Programa de Microbiología y Micología, ICBM, Facultad de MedicinaUniversidad de ChileSantiagoChile
  2. 2.Laboratorio Clínica DávilaSantiagoChile
  3. 3.Escuela de Tecnología MédicaUniversidad MayorSantiagoChile
  4. 4.Departamento de Ciencias Biológicas Animales, Facultad de Ciencias Veterinarias y Pecuarias, Centro Biotecnológico BiovetecUniversidad de ChileSantiagoChile
  5. 5.Departamento de Obstetricia y Ginecología, Hospital ClínicoUniversidad de ChileSantiagoChile
  6. 6.Center for Integrative Medicine and Innovative Science, Facultad de MedicinaUniversidad Andrés BelloSantiagoChile
  7. 7.Laboratoire de BiologieConservatoire National des Arts et MétiersParisFrance

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