Mycopathologia

, Volume 182, Issue 3–4, pp 305–313 | Cite as

In Vitro Antifungal Susceptibility of Neoscytalidium dimidiatum Clinical Isolates from Malaysia

  • Jasper Elvin James
  • Jacinta Santhanam
  • Mei Chen Lee
  • Choon Xian Wong
  • Parameswari Sabaratnam
  • Hamidah Yusoff
  • Mohd Nizam Tzar
  • Mohd Fuat Abdul Razak
Article

Abstract

Neoscytalidium dimidiatum is an opportunistic fungus causing cutaneous infections mostly, which are difficult to treat due to antifungal resistance. In Malaysia, N. dimidiatum is associated with skin and nail infections, especially in the elderly. These infections may be mistaken for dermatophyte infections due to similar clinical appearance. In this study, Neoscytalidium isolates from cutaneous specimens, identified using morphological and molecular methods (28 Neoscytalidium dimidiatum and 1 Neoscytalidium sp.), were evaluated for susceptibility towards antifungal agents using the CLSI broth microdilution (M38-A2) and Etest methods. Amphotericin B, voriconazole, miconazole and clotrimazole showed high in vitro activity against all isolates with MIC ranging from 0.0313 to 1 µg/mL. Susceptibility towards fluconazole and itraconazole was noted in up to 10% of isolates, while ketoconazole was inactive against all isolates. Clinical breakpoints for antifungal drugs are not yet available for most filamentous fungi, including Neoscytalidium species. However, the results indicate that clinical isolates of N. dimidiatum in Malaysia were sensitive towards miconazole, clotrimazole, voriconazole and amphotericin B, in vitro.

Keywords

Neoscytalidium dimidiatum Antifungal susceptibility Malaysia 

Notes

Acknowledgements

We would like to thank Pharmaniaga (Malaysia) for providing antifungal agents for this study. This work was fully funded by a Research University Grant UKM-GUP-2011-118 from Universiti Kebangsaan Malaysia.

Compliance with ethical standards

This study is in compliance with institutional ethical requirements of Universiti Kebangsaan Malaysia.

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Jasper Elvin James
    • 1
  • Jacinta Santhanam
    • 1
  • Mei Chen Lee
    • 1
  • Choon Xian Wong
    • 1
  • Parameswari Sabaratnam
    • 2
  • Hamidah Yusoff
    • 3
  • Mohd Nizam Tzar
    • 3
  • Mohd Fuat Abdul Razak
    • 2
  1. 1.Biomedical Science Programme, Faculty of Health SciencesUniversiti Kebangsaan MalaysiaKuala LumpurMalaysia
  2. 2.Bacteriology UnitInstitute for Medical ResearchKuala LumpurMalaysia
  3. 3.Department of Medical Microbiology and ImmunologyUniversiti Kebangsaan Malaysia Medical CentreCherasMalaysia

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