, Volume 182, Issue 3–4, pp 365–370 | Cite as

Isolation of Cryptococcus gattii from a Castanopsis argyrophylla tree hollow (Mai-Kaw), Chiang Mai, Thailand

  • Kantarawee Khayhan
  • Ferry Hagen
  • Treepradab Norkaew
  • Tanpalang Puengchan
  • Teun Boekhout
  • Pojana Sriburee


The pathogenic yeast Cryptococcus gattii was isolated from a tree hollow of a Castanopsis argyrophylla King ex Hook.f. (Fagaceae) in Chiang Mai, Thailand. Molecular characterization with amplified fragment length polymorphism analysis and multi-locus sequence typing showed that this isolate belonged to genotype AFLP4/VGI representing C. gattii sensu stricto. Subsequent comparison of the environmental isolate with those from clinical samples from Thailand showed that they grouped closely together in a single cluster.


Cryptococcus gattii AFLP genotyping Multi-locus sequence typing Environmental sampling Thailand 


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Copyright information

© Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  • Kantarawee Khayhan
    • 1
    • 2
  • Ferry Hagen
    • 3
  • Treepradab Norkaew
    • 4
  • Tanpalang Puengchan
    • 5
  • Teun Boekhout
    • 2
    • 6
  • Pojana Sriburee
    • 4
  1. 1.Department of Microbiology and Parasitology, Faculty of Medical SciencesUniversity of PhayaoMuangThailand
  2. 2.CBS-KNAW Fungal Biodiversity CentreBasidiomycete and Yeast ResearchUtrechtThe Netherlands
  3. 3.Department of Medical Microbiology and Infectious DiseasesCanisius-Wilhelmina HospitalNijmegenThe Netherlands
  4. 4.Department of Microbiology, Faculty of MedicineChiang Mai UniversityChiang MaiThailand
  5. 5.Queen Sirikit Botanic GardenChiang MaiThailand
  6. 6.Institute of Biodiversity and Ecosystem Dynamics (IBEA)University of AmsterdamAmsterdamThe Netherlands

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