Isolation and Characterization of an Unknown Chrysosporium sp. Producing Subcutaneous Mycosis in an Immunocompromised Patient
- 144 Downloads
We report a case of subcutaneous fungal abscess over the great toe caused by a keratinophilic fungus, an unknown Chrysosporium sp., in a 60-year-old diabetic female who was treated successfully with oral fluconazole. The fungus was isolated from aspirated pus, and septate hyphae were seen in fine needle aspiration cytology. Ovoid- to club-shaped hyaline one-celled conidia (aleuriconidia) with broad truncated bases were seen, and sequencing of ITS1-5.8S-ITS2 rDNA revealed belonging to the order Onygenales and most closely related to Chrysosporium spp. isolated from a fowl. Of the 65 species within the genus Chrysosporium, very few have been reported as pathogenic.
KeywordsChrysosporium sp. Keratinophilic Subcutaneous DNA sequencing
We thank the staff of Centre of Advance Research in Medical Mycology, WHO Collaborating Centre, National Culture Collection of Pathogenic Fungi, Postgraduate institute of medical education and research (PGIMER), Chandigarh, India, for their assistance in identification of the fungus till species level.
Conflict of interest
The authors declare they have no conflict of interest.
All procedures performed in the studies involving human participants were in accordance with the ethical standards of the institution and/or national research committee and with the Declaration of Helsinki 1964 and its amendments or comparable ethical standards.
Informed consent was obtained.
- 1.Sigler L. Chrysosporium and molds resembling dermatophytes. In: Kane J, editor. Laboratory handbook of dermatophytes: a clinical guide and laboratory manual of dermatophytes and other filamentous fungi from skin, hair and nails. Belmont: Star Publishing Company; 1997. p. 261–311.Google Scholar
- 8.Seifert K, Morgan-Jones G, Gams W, Kenderick B. The genera of Hyphomycetes. Ultrecht: Centraalbureau voor Schimmelcultures; 2011.Google Scholar
- 9.Vidal P, Vinuesa MDLA, Sanchez-Puelles JM, Guarro J. Phylogeny of the anamorphic genus Chrysosporium and related taxa based on rDNA internal transcribed spacer sequences. In: Kushwaha RKS, Guarro J, editors. Biology of dermatophytes and other keratinophilic fungi. 17th ed. Bilbao: Revista Iberoamericana de Micologia; 2000. p. 22–9.Google Scholar
- 11.Rizzo L, Sutton DA, Wiederhold NP, Thompson EH, Friedman R, Wickes BL, Cano-Lira JF, Stchigel AM, Guarro J. Isolation and characterisation of the fungus Spiromastix asexualis sp. nov. from discospondylitis in a German Shepherd dog, and review of Spiromastix with the proposal of the new order Spiromastixales (Ascomycota). Mycoses. 2014;57:419–28.CrossRefPubMedGoogle Scholar