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Mycopathologia

, Volume 176, Issue 3–4, pp 299–302 | Cite as

Serological Survey of Paracoccidioidomycosis in Cats

  • Gabriela Gonçalves de Oliveira
  • Donizeti Rodrigues Belitardo
  • Mara Regina Stipp Balarin
  • Roberta Lemos Freire
  • Zoilo Pires de Camargo
  • Mario Augusto Ono
Article

Abstract

The objective of the present study was to evaluate infection of cats by Paracoccidioides brasiliensis. Serum samples of 136 cats from rural (n = 86) and urban areas (n = 50) were analyzed by indirect ELISA and immunodiffusion test using P. brasiliensis gp43 and exoantigen as antigens, respectively, and an overall reactivity of 31.6 % was observed by ELISA although no reactivity was detected by immunodiffusion. The positivity observed in animals living in rural areas (48.8 %) with free access to soil was significantly higher (P < 0.0001) than among urban animals (2 %) with limited access to soil, although no significant difference was observed in relation to age or sex. The high rates of positivity observed in cats from rural areas suggest that not diagnosed cases of this mycosis may be occurring in cats living in endemic areas for human paracoccidioidomycosis. This is the first report showing serological evidence of P. brasiliensis infection in cats.

Keywords

Epidemiology Paracoccidioides brasiliensis Feline Systemic mycosis 

Notes

Acknowledgments

The authors thank the CNPq, CAPES and the Araucaria Foundation for financial support, the CNPq for the productivity fellowship granted to MA Ono and ZP Camargo and the veterinarian Adilson Carlos Lúcio for help with collecting the blood samples.

References

  1. 1.
    Brummer E, Castañeda E, Restrepo A. Paracoccidioidomycosis: an update. Clin Microbiol Rev. 1993;6:89–117.PubMedGoogle Scholar
  2. 2.
    Wanke B, Londero AT. Epidemiology and paracoccidioidomycosis infection. In: Franco MF, Lacaz CS, Restrepo-Moreno A, Del Negro G, editors. Paracoccidioidomycosis. Boca Raton: CRC Press; 1994. p. 109–20.Google Scholar
  3. 3.
    Conti-Diaz IA, Alvarez BJ, Gezuele E, Gonzalez MH, Duarte J, Falcon J. Intradermal reaction survey with paracoccidioidin and histoplasmin in horses. Rev Inst Med Trop São Paulo. 1972;14:372–6.PubMedGoogle Scholar
  4. 4.
    Costa EO, Fava-Netto C. Contribution to the epidemiology of paracoccidioidomycosis and histoplasmosis in the State of São Paulo, Brazil. Paracoccidioidin and Histoplasmin intradermic tests in domestic animals. Sabouraudia. 1978;16:93–101.PubMedCrossRefGoogle Scholar
  5. 5.
    Corte AC, Itano EN, Freire RL, Camargo ZP, Ono MA. Detection of antibodies to Paracoccidioides brasiliensis in horses from northern region of Paraná State. Semina-Ciências Agrárias. 2009;30:441–6.Google Scholar
  6. 6.
    Silveira LH, Paes RC, Medeiros EV, Itano EN, Camargo ZP, Ono MA. Occurrence of antibodies to Paracoccidioides brasiliensis in dairy cattle from Mato Grosso do Sul, Brazil. Mycopathologia. 2008;165:367–71.PubMedCrossRefGoogle Scholar
  7. 7.
    Corte AC, Svoboda WK, Navarro IT, Freire RL, Malanski LS, Shiozawa MM, Ludwig G, Aguiar LM, Passos FC, Maron A, Camargo ZP, Itano EN, Ono MA. Paracoccidioidomycosis in wild monkeys from Paraná State, Brazil. Mycopathologia. 2007;64:225–8.CrossRefGoogle Scholar
  8. 8.
    Costa EO, Diniz LS, Fava Netto C, Arruda C, Dagli ML. Delayed hypersensitivity test with paracoccidioidin in captive Latin American wild mammals. J Med Vet Mycol. 1995;33:39–42.PubMedCrossRefGoogle Scholar
  9. 9.
    Mós EN, Fava-Netto C. Contribuição ao estudo da paracoccidoidomicose -Possível papel dos cães: Estudo sorológico e anatomo-patológico. Rev Inst Med Trop São Paulo. 1974;16:154–9.Google Scholar
  10. 10.
    Ono MA, Bracarense APFRL, Morais HSA, Trapp SM, Belitardo DR, Camargo ZP. Canine paracoccidioidomycosis: a seroepidemiologic study. Med Mycol. 2001;39:277–82.PubMedGoogle Scholar
  11. 11.
    Silveira LH, Domingos IH, Kouchi K, Itano EN, Silva EA, Landgraf VO, Werneck SM, Camargo ZP, Ono MA. Serological detection of antibodies against Paracoccidioides brasiliensis in dogs with leishmaniasis. Mycopathologia. 2006;162:325–9.PubMedCrossRefGoogle Scholar
  12. 12.
    Fontana FF, Santos CTB, Esteves FM, Rocha A, Fernandes GF, Amaral CC, Domingues MA, Camargo ZP, Silva-Vergara ML. Seroepidemiological survey of paracoccidioidomycosis infection among urban and rural dogs from Uberaba, Minas Gerais, Brazil. Mycopathologia. 2010;169:159–65.PubMedCrossRefGoogle Scholar
  13. 13.
    Corte AC, Gennari SM, Labruna MB, Camargo LMA, Itano EN, Freire RL, Camargo ZP, Ono MA. Paracoccidioides brasiliensis infection in dogs from Western Brazilian Amazon. Pesq Vet Bras. 2012;32(7):649–52.CrossRefGoogle Scholar
  14. 14.
    Oliveira GG, Silveira LH, Itano EN, Soares RM, Freire RF, Watanabe MAE, Ono MA. Serological evidence of Paracoccidioides brasiliensis in chickens from Paraná and Mato Grosso do Sul States, Brazil. Mycopathologia. 2011;171:197–202.PubMedCrossRefGoogle Scholar
  15. 15.
    Oliveira GG, Navarro IT, Freire RL, Belitardo DR, Silveira LH, Camargo ZP, Itano EN, Ono MA. Serological survey of paracoccidioidomycosis in sheep. Mycopathologia. 2012;173(1):63–8.PubMedCrossRefGoogle Scholar
  16. 16.
    Ferreira JB, Navarro IT, Freire RL, Oliveira GG, Omori AM, Belitardo DR, Itano EN, Camargo ZP, Ono MA. Evaluation of Paracoccidioides brasiliensis Infection in dairy goats. Mycopathologia. 2013;176:95–9.Google Scholar
  17. 17.
    Richini-Pereira VB, Bosco SM, Griese J, Theodoro RC, Macoris SA, Silva RJ, Barrozo L, Tavares PM, Zancopé-Oliveira RM, Bagagli E. Molecular detection of Paracoccidioides brasiliensis in road-killed wild animals. Med Mycol. 2008;46:35–40.PubMedCrossRefGoogle Scholar
  18. 18.
    Naiff RD, Ferreira LC, Barret TV, Naif MF, Arias JR. Paracoccidioidomicose enzoótica em tatus (Dasypus novemcinctus) no estado do Pará. Rev Inst Med Trop S Paulo. 1986;28:19–27.PubMedCrossRefGoogle Scholar
  19. 19.
    Bagagli E, Sano A, Coelho KI, Alquati S, Miyaji M, Camargo ZP, Gomes GM, Franco M, Montenegro MR. Isolation of Paracoccidioides brasiliensis from armadillos (Dasypus novemcinctus) captured in an area of paracoccidioidomycosis. Am J Trop Med Hyg. 1998;58:505–12.PubMedGoogle Scholar
  20. 20.
    Corredor GG, Castaño JH, Peralta LA, Díez S, Arango M, Mcwen J, Restrepo A. Isolation of Paracoccidioides brasiliensis from the nine-banded armadillo Dasypus novemcinctus, in an endemic area for paracoccidioidomycosis in Colombia. Rev Iberoam Micol. 1999;16:216–20.PubMedGoogle Scholar
  21. 21.
    Gonzales JF, Montiel NA, Maass RL. First report on the diagnosis of encephalic and urinary paracoccidioidomycosis in a cat. J Fel Med Surg. 2010;12:659–62.CrossRefGoogle Scholar
  22. 22.
    Farias MR, Condas LAZ, Ribeiro MG, Bosco SM, Muro MD, Werner J, Theodoro RC, Bagagli E, Marques SA, Franco M. Paracoccidioidomycosis in a dog: case report of generalized lymphadenomegaly. Mycopathologia. 2011;172(2):147–52.PubMedCrossRefGoogle Scholar
  23. 23.
    Trejo-Chávez A, Ramírez-Romero R, Ancer-Rodríguez J, Nevárez-Garza AM, Rodríguez-Tovar E. Disseminated paracoccidioidomycosis in a Southern two-toed sloth (Choloepus didactylus). J Comp Pathol. 2011;144:231–4.PubMedCrossRefGoogle Scholar
  24. 24.
    Batista AC, Shome SK, Santos FM. Pathogenicity of Paracoccidioides brasiliensis isolated from soil. Recife. Publ Univ Recife Inst Micol. 1962;372:1–27.Google Scholar
  25. 25.
    Negroni P. El Paracoccidioides brasiliensis vive saprofiticamente en el suelo argentino. Prensa Méd Argent. 1966;53:2831–2.Google Scholar
  26. 26.
    Albornoz MB. Isolation of Paracoccidioides brasiliensis from rural soil in Venezuela. Sabouraudia. 1971;9:248–53.PubMedCrossRefGoogle Scholar
  27. 27.
    Silva-Vergara ML, Martinez R, Chadu A, Madeira M, Freitas-Silva G, Maffei CM. Isolation of a Paracoccidioides brasiliensis strain from the soil of a coffee plantation in Ibiá, State of Minas Gerais, Brazil. Med Mycol. 1998;36:37–42.PubMedCrossRefGoogle Scholar
  28. 28.
    Camargo ZP, Unterkircher CS, Campoy SP, Travassos LR. Production of Paracoccidioides brasiliensis exoantigens for immunodiffusion tests. J Clin Microbiol. 1988;26:2147–51.PubMedGoogle Scholar
  29. 29.
    Puccia R, Travassos LR. The 43-kDa glycoprotein from the human pathogen Paracoccidioides brasiliensis and its deglycosylated form: excretion and susceptibility to proteolysis. Arch Biochem Biophys. 1991;289:298–302.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Gabriela Gonçalves de Oliveira
    • 1
  • Donizeti Rodrigues Belitardo
    • 1
  • Mara Regina Stipp Balarin
    • 2
  • Roberta Lemos Freire
    • 2
  • Zoilo Pires de Camargo
    • 3
  • Mario Augusto Ono
    • 1
  1. 1.Departamento de Ciências Patológicas, Centro de Ciências BiológicasUniversidade Estadual de LondrinaLondrinaBrazil
  2. 2.Departamento de Medicina Veterinária PreventivaUniversidade Estadual de LondrinaLondrinaBrazil
  3. 3.Disciplina de Biologia CelularUniversidade Federal de São PauloSão PauloBrazil

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