, Volume 176, Issue 1–2, pp 165–169 | Cite as

Antifungal Activities of Diphenyl Diselenide alone and in Combination with Fluconazole or Amphotericin B against Candida glabrata

  • Laura Bedin Denardi
  • Débora Alves Nunes Mario
  • Érico Silva de Loreto
  • Cristina Wayne Nogueira
  • Janio Morais Santurio
  • Sydney Hartz Alves


Here, we evaluated combinations of diphenyl diselenide [(PhSe)2] with fluconazole and amphotericin B in a checkerboard assay against clinical Candida glabrata strains. Minimal inhibitory concentration (geometric mean) ranged from 0.25 to >64 (5.16 μg/mL) for (PhSe)2, 1 to 32 (5.04 μg/mL) for fluconazole and 0.06 to 0.5 (0.18 μg/mL) for amphotericin B. Synergistic (76.66 %) and indifferent (23.34 %) interactions were observed for (PhSe)2 + amphotericin B combination. (PhSe)2 + fluconazole combination demonstrated indifferent (50 %) and antagonistic (40 %) interactions, whereas synergistic interactions were observed in 10 % of the isolates. New experimental in vivo protocols are necessary and will promote a better understanding of the antimicrobial activity of (PhSe)2 against C. glabrata and its use as an adjuvant therapy with antifungal agents.


Antifungal drug combination Candidaglabrata Diphenyl diselenide 



Érico Silva de Loreto is financially supported by fellowships from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil (PNPD-CAPES).


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Laura Bedin Denardi
    • 1
  • Débora Alves Nunes Mario
    • 1
  • Érico Silva de Loreto
    • 2
  • Cristina Wayne Nogueira
    • 3
  • Janio Morais Santurio
    • 2
  • Sydney Hartz Alves
    • 1
  1. 1.Programa de Pós-Graduação em Ciências Farmacêuticas, Centro de Ciências da SaúdeUniversidade Federal de Santa Maria (UFSM)Santa MariaBrazil
  2. 2.Programa de Pós-Graduação em Farmacologia, Centro de Ciências da SaúdeUniversidade Federal de Santa Maria (UFSM)Santa MariaBrazil
  3. 3.Programa de Pós-Graduação em Bioquímica Toxicológica, Centro de Ciências da SaúdeUniversidade Federal de Santa Maria (UFSM)Santa MariaBrazil

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