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Antifungal Activities of Diphenyl Diselenide alone and in Combination with Fluconazole or Amphotericin B against Candida glabrata

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Abstract

Here, we evaluated combinations of diphenyl diselenide [(PhSe)2] with fluconazole and amphotericin B in a checkerboard assay against clinical Candida glabrata strains. Minimal inhibitory concentration (geometric mean) ranged from 0.25 to >64 (5.16 μg/mL) for (PhSe)2, 1 to 32 (5.04 μg/mL) for fluconazole and 0.06 to 0.5 (0.18 μg/mL) for amphotericin B. Synergistic (76.66 %) and indifferent (23.34 %) interactions were observed for (PhSe)2 + amphotericin B combination. (PhSe)2 + fluconazole combination demonstrated indifferent (50 %) and antagonistic (40 %) interactions, whereas synergistic interactions were observed in 10 % of the isolates. New experimental in vivo protocols are necessary and will promote a better understanding of the antimicrobial activity of (PhSe)2 against C. glabrata and its use as an adjuvant therapy with antifungal agents.

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Acknowledgments

Érico Silva de Loreto is financially supported by fellowships from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil (PNPD-CAPES).

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Authors and Affiliations

  1. Programa de Pós-Graduação em Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria (UFSM), Campus UFSM, Prédio 20, sala 4139, Santa Maria, RS, 97105-900, Brazil

    Laura Bedin Denardi, Débora Alves Nunes Mario & Sydney Hartz Alves

  2. Programa de Pós-Graduação em Farmacologia, Centro de Ciências da Saúde, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil

    Érico Silva de Loreto & Janio Morais Santurio

  3. Programa de Pós-Graduação em Bioquímica Toxicológica, Centro de Ciências da Saúde, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil

    Cristina Wayne Nogueira

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  1. Laura Bedin Denardi
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  2. Débora Alves Nunes Mario
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  3. Érico Silva de Loreto
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Correspondence to Laura Bedin Denardi.

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Denardi, L.B., Mario, D.A.N., de Loreto, É.S. et al. Antifungal Activities of Diphenyl Diselenide alone and in Combination with Fluconazole or Amphotericin B against Candida glabrata . Mycopathologia 176, 165–169 (2013). https://doi.org/10.1007/s11046-013-9672-x

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  • DOI: https://doi.org/10.1007/s11046-013-9672-x

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