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Mycopathologia

, Volume 176, Issue 3–4, pp 295–297 | Cite as

Reproducibility of Positive Results for the Detection of Serum Galactomannan by Platelia™ Aspergillus EIA

  • Kelly C. M. C. Pedroza
  • Sócrates B. de Matos
  • Daniel L. de Moura
  • Mônica B. B. Oliveira
  • Marco A. S. Araújo
  • Roberto J. M. Nascimento
  • Fernanda W. M. Lima
Article

Abstract

Galactomannan (GM) was recently included in consensus guidelines as an indirect mycological criterion for the diagnosis of invasive aspergillosis. Currently, there is an enzyme immunoassay available to detect GM in biological samples, the Platelia™ Aspergillus EIA. In this study, the reproducibility of positive results obtained using this assay was evaluated using serum samples from neutropenic patients. A trend toward lower values was observed, and 55 %(27/49) of positive results were negative after retesting. A low reproducibility of positive results for the detection of GM in serum was observed.

Keywords

Aspergillosis ELISA Galactomannan Reproducibility Serum 

Notes

Acknowledgments

FAPESB, Fundação de Amparo à Pesquisa do Estado da Bahia; CAPES, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; ISID/Federal University of Bahia.

References

  1. 1.
    Racil Z, Kocmanova I, Toskova M, Buresova L, Weinbergerova B, Lengerova M, et al. Galactomannan detection in bronchoalveolar lavage fluid for the diagnosis of invasive aspergillosis in patients with hematological diseases-the role of factors affecting assay performance. IJDI. 2011;15:874–81.Google Scholar
  2. 2.
    Hadrich I, Makni F, Cheikhrouhou F, Neji S, Amouri I, Sellami H, Trabelsi H, Bellaaj H, Elloumi M, Ayadi A. Clinical utility and prognostic value of galactomannan in neutropenic patients with invasive aspergillosis. Pathol Biol. 2012;60(6):357–61.Google Scholar
  3. 3.
    De Pauw B, Walsh TJ, Donnelly JP, Stevens DA, Edwards JE, Calandra T, et al. Revised definitions of invasive fungal disease from the European organization for research and treatment of cancer/invasive fungal infections cooperative group and the national institute of allergy and infectious diseases mycoses study group (EORTC/MSG) consensus group. Clin Infect Dis. 2008;46:1813–21.PubMedCrossRefGoogle Scholar
  4. 4.
    ANVISA. Resolução nº3.288, de 19 de outubro de 2007. Available at http://www.anvisa.gov.br/legis/suplemento/221007_suplemento_1.pdf. Accessed on 12-Oct-2012.
  5. 5.
    Aquino VR, Goldani EZ, Pasqualotto AC. Update on the contribution of galactomannan for the diagnosis of invasive aspergillosis. Mycopathologia. 2007;163:191–202.PubMedCrossRefGoogle Scholar
  6. 6.
    Asano-Mori Y, Kanda Y, Oshima K, Kako S, Shinohara A, Nakasone H, et al. False-positive Aspergillus galactomannan antigenaemia after haematopoietic stem cell transplantation. JAC. 2008;61:411–6.PubMedGoogle Scholar
  7. 7.
    Maertens JA, Klont R, Masson C, Theunissen K, Meersseman W, Lagrou K, et al. Optimization of the cutoff value for the Aspergillus double-sandwich enzyme immunoassay. CID. 2007;44:1329–33.CrossRefGoogle Scholar
  8. 8.
    Bizzini A, Marchetti O, Meylan P. Response to: lack of intra-laboratory reproducibility in using platelia Aspergillus enzyme immunoassay test for detection of Aspergillus galactomannan antigen. Transpl Infect Dis. 2012;14:218–9.PubMedCrossRefGoogle Scholar
  9. 9.
    Oren I, Avidor I, Sprecher H. Lack of intra-laboratory reproducibility in using platelia Aspergillus enzyme immunoassay test for detection of Aspergillus galactomannan antigen. Transpl Infect Dis. 2012;14:107–9.PubMedCrossRefGoogle Scholar
  10. 10.
    Furfaro E, Mikulska M, Miletich F, Viscoli C. Galactomannan: testing the same sample twice? Transpl Infect Dis. 2012;14:38–9.CrossRefGoogle Scholar
  11. 11.
    Nucci M, Nouer SA, Grazziutti M, Kumar NS, Barlogie B, Anaissie E. Probable invasive aspergillosis without prespecified radiologic findings: proposal for inclusion of a new category of aspergillosis and implications for studying novel therapies. CID. 2010;51:1273–80.CrossRefGoogle Scholar
  12. 12.
    Mennink-Kersten MASH, Donnelly JP, Verweij PE. Detection of circulating galactomannan for the diagnosis and management of invasive aspergillosis. Lancet Infect Dis. 2004;4(6):349–57.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Kelly C. M. C. Pedroza
    • 1
  • Sócrates B. de Matos
    • 1
  • Daniel L. de Moura
    • 1
  • Mônica B. B. Oliveira
    • 2
  • Marco A. S. Araújo
    • 2
  • Roberto J. M. Nascimento
    • 3
  • Fernanda W. M. Lima
    • 1
  1. 1.Immunology Service of Infectious Diseases (ISID)Federal University of BahiaSalvadorBrazil
  2. 2.Hematology ServiceFederal University of BahiaSalvadorBrazil
  3. 3.Laboratory of Immunology and Molecular BiologyFederal University of BahiaSalvadorBrazil

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