Advertisement

Mycopathologia

, Volume 176, Issue 1–2, pp 95–99 | Cite as

Evaluation of Paracoccidioides brasiliensis Infection in Dairy Goats

  • Jacqueline B. Ferreira
  • Italmar T. Navarro
  • Roberta L. Freire
  • Gabriela G. Oliveira
  • Aline M. Omori
  • Donizeti R. Belitardo
  • Eiko N. Itano
  • Zoilo P. Camargo
  • Mario A. Ono
Article

Abstract

Paracoccidioides brasiliensis is the etiological agent of paracoccidioidomycosis, a systemic mycosis that affects mainly rural workers in Brazil and other Latin American countries. The participation of domestic and wild animal species in the ecoepidemiology of paracoccidioidomycosis is not well understood. The objective of this study was to evaluate P. brasiliensis infection in dairy goats. The humoral immune response to the gp43 antigen, the main antigen used for paracoccidioidomycosis serodiagnosis and seroepidemiology, was evaluated in two goats immunized with inactivated P. brasiliensis yeast cells. Both animals produced antibodies against the P. brasiliensis gp43 antigen, detected by ELISA, 2 weeks after immunization. A total of 202 goat serum samples were analyzed by ELISA and the immunodiffusion test using P. brasiliensis gp43 and exoantigen as antigens. The seropositivity observed by ELISA was 26.2 % although no reactivity was detected by immunodiffusion. The animals over 18 months of age showed significantly higher positivity (40 %) than animals aged 6–18 months (14.8 %) and 0–6 months (2.6 %). Taking into account that cross-reactivity may occur with other pathogens, the serum samples were also analyzed by ELISA using Histoplasma capsulatum exoantigen as antigen and the positivity observed was 14.3 %. The low correlation (0.267) observed between reactivity to P. brasiliensis gp43 and H. capsulatum exoantigen suggests co-infection rather than cross-reactivity. This is the first report showing serological evidence of P. brasiliensis infection in goats and reinforces that domestic animals are useful epidemiological markers of paracoccidioidomycosis.

Keywords

Epidemiology Paracoccidioidomycosis Caprine 

Notes

Acknowledgments

The authors thank the CNPq, CAPES and the Araucaria Foundation for financial support and the CNPq for the productivity fellowship granted to MA Ono, IT Navarro, EN Itano and ZP Camargo.

References

  1. 1.
    Brummer E, Castaneda E, Restrepo A. Paracoccidioidomycosis: an update. Clin Microbiol Rev. 1993;6:89–117.PubMedGoogle Scholar
  2. 2.
    El Negroni P. Paracoccidioides brasiliensis vive saprofiticamente en el suelo argentino. Prensa Méd Argent. 1966;53:2831–2.Google Scholar
  3. 3.
    Albornoz MB. Isolation of Paracoccidioides brasiliensis from rural soil in Venezuela. Sabouraudia. 1971;9:248–53.PubMedCrossRefGoogle Scholar
  4. 4.
    Silva-Vergara ML, Martinez R, Chadu A, Madeira M, Freitas-Silva G, Maffei CML. Isolation of Paracoccidioides brasiliensis strain from the soil of a coffee plantation in Ibiá, State of Minas Gerais, Brazil. Med Mycol. 1998;36:37–42.PubMedCrossRefGoogle Scholar
  5. 5.
    Oliveira GG, Silveira LH, Itano EN, Soares RM, Freire RF, Watanabe MAE, Ono MA. Serological evidence of Paracoccidioides brasiliensis infection in chickens from Paraná and Mato Grosso do Sul States, Brazil. Mycopathologia. 2011;171:197–202.PubMedCrossRefGoogle Scholar
  6. 6.
    Grose E, Tamsitt JR. Paracoccidioides brasiliensis recovered from intestinal tract of three bats (Artibeus lituratus) in Colombia, S.A. Sabouraudia. 1965;4:124–5.PubMedCrossRefGoogle Scholar
  7. 7.
    Naiff RD, Ferreira LCL, Barret TV, Naif MF, Arias JR. Paracoccidioidomicose enzoótica em tatus (Dasypus novemcinctus) no estado do Pará. Rev Inst Med Trop Sao Paulo. 1986;28:19–27.PubMedCrossRefGoogle Scholar
  8. 8.
    Bagagli E, Sano A, Coelho KI, Alquati S, Miyaji M, Camargo ZP, Gomes GM, Franco M, Montenegro MR. Isolation of Paracoccidioides brasiliensis from armadillos (Dasypus novemcinctus) captured in an area of paracoccidioidomycosis. Am J Trop Med Hyg. 1998;58:505–12.PubMedGoogle Scholar
  9. 9.
    Corredor GG, Castaño JH, Peralta LA, Díez S, Arango M, McEwen J, Restrepo A. Isolation of Paracoccidioides brasiliensis from the nine-banded armadillo Dasypus novemcinctus, in an endemic area for paracoccidioidomycosis in Colombia. Rev Iberoam Micol. 1999;16:216–20.PubMedGoogle Scholar
  10. 10.
    Farias MR, Condas LAZ, Ribeiro MG, Bosco SMG, Muro MD, Werner J, Theodoro RC, Bagagli E, Marques SA, Franco M. Paracoccidioidomycosis in a dog: case report of generalized lymphadenomegaly. Mycopathologia. 2011;172:147–52.PubMedCrossRefGoogle Scholar
  11. 11.
    Gonzales JF, Montiel NA, Maass RL. First report on the diagnosis of encephalic and urinary paracoccidioidomycosis in a cat. J Feline Med Surg. 2010;12:659–62.CrossRefGoogle Scholar
  12. 12.
    Mós EN, Fava-Netto C. Contribuição ao estudo da paracoccidoidomicose. I-Possível papel dos cães: estudo sorológico e anatomo-patológico. Rev Inst Med Trop Sao Paulo. 1974;16:154–9.Google Scholar
  13. 13.
    Ono MA, Bracarense APFRL, Morais HSA, Trapp SM, Belitardo DR, Camargo ZP. Canine paracoccidioidomycosis: a seroepidemiologic study. Med Mycol. 2001;39:277–82.PubMedGoogle Scholar
  14. 14.
    Silveira LH, Domingos IH, Kouchi K, Itano EN, Silva EA, Landgraf VO, Werneck SM, Camargo ZP, Ono MA. Serological detection of antibodies against Paracoccidioides brasiliensis in dogs with leishmaniasis. Mycopathologia. 2006;162:325–9.PubMedCrossRefGoogle Scholar
  15. 15.
    Fontana FF, Santos CTB, Esteves FM, Rocha A, Fernandes GF, Amaral CC, Domingues MA, Camargo ZP, Silva-Vergara ML. Seroepidemiological survey of paracoccidioidomycosis infection among urban and rural dogs from Uberaba, Minas Gerais, Brazil. Mycopathologia. 2010;169:159–65.PubMedCrossRefGoogle Scholar
  16. 16.
    Silveira LH, Paes RCS, Medeiros EV, Itano EM, Camargo ZP, Ono MA. Occurrence of antibodies to Paracoccidioides brasiliensis in dairy cattle from Mato Grosso do Sul, Brazil. Mycopathologia. 2008;165:367–71.PubMedCrossRefGoogle Scholar
  17. 17.
    Corte AC, Itano EN, Freire RL, Camargo ZP, Ono MA. Detection of antibodies to Paracoccidioides brasiliensis in horses from northern Region of Paraná State. Semina-Ciências Agrárias. 2009;30:441–6.Google Scholar
  18. 18.
    Oliveira GG, Navarro IT, Freire RL, Belitardo DR, Silveira LH, Camargo ZP, Itano EN, Ono MA. Serological survey of Paracoccidioidomycosis in sheep. Mycopathologia. 2012;173:63–8.PubMedCrossRefGoogle Scholar
  19. 19.
    Fernandes GF, Deps P, Tomimori-Yamashita J, Camargo ZP. IgM and IgG antibody response to Paracoccidioides brasiliensis in naturally infected wild armadillos (Dasypus novemcinctus). Med Mycol. 2004;42:363–8.PubMedCrossRefGoogle Scholar
  20. 20.
    Corte AC, Svoboda WK, Navarro IT, Freire RL, Malanski LS, Shiozawa MM, Ludwig G, Aguiar LM, Passos FC, Maron A, Camargo ZP, Itano EN, Ono MA. Paracoccidioidomycosis in wild monkeys from Paraná State, Brazil. Mycopathologia. 2007;164:225–8.PubMedCrossRefGoogle Scholar
  21. 21.
    Richini-Pereira VB, Bosco SM, Griese J, Theodoro RC, Macoris SA, Silva RJ, Barrozo L, Tavares PM, Zancopé-Oliveira RM, Bagagli E. Molecular detection of Paracoccidioides brasiliensis in road-killed wild animals. Med Mycol. 2008;46:35–40.PubMedCrossRefGoogle Scholar
  22. 22.
    Bittencourt JI, de Oliveira RM, Coutinho ZF. Paracoccidioidomycosis mortality in the State of Paraná, Brazil, 1980/1998. Cad Saude Publica. 2005;21:1856–64.PubMedCrossRefGoogle Scholar
  23. 23.
    Ono MA, Itano EN, Mizuno LT, Mizuno EH, Camargo ZP. Inhibition of Paracoccidioides brasiliensis by pesticides: is this a partial explanation for the difficulty in isolating this fungus from the soil? Med Mycol. 2002;40:493–9.PubMedGoogle Scholar
  24. 24.
    Camargo ZP, Unterkircher CS, Campoy SP, Travassos LR. Production of Paracoccidioides brasiliensis exoantigens for immunodiffusion tests. J Clin Microbiol. 1988;26:2147–51.PubMedGoogle Scholar
  25. 25.
    Puccia R, Travassos LR. The 43-kDa glycoprotein from the human pathogen Paracoccidioides brasiliensis and its deglycosylated form: excretion and susceptibility to proteolysis. Arch Biochem Biophys. 1991;289:298–302.PubMedCrossRefGoogle Scholar
  26. 26.
    Ono MA, Kishima MO, Itano EN, Bracarense AP, Camargo ZP. Experimental paracoccidioidomycosis in dogs. Med Mycol. 2003;41:265–8.PubMedCrossRefGoogle Scholar
  27. 27.
    Eisele RC, Juliani LC, Belitardo DR, Itano EN, Estevão D, Bracarense AP, Camargo ZP, Ono MA. Immune response in dogs experimentally infected with Paracoccidioides brasiliensis. Med Mycol. 2004;42:549–53.PubMedCrossRefGoogle Scholar
  28. 28.
    Camargo ZP, Baruzzi RG, Maeda SM, Floriano MC. Antigenic relationship between Loboa loboi and Paracoccidioides brasiliensis as shown by serological methods. Med Mycol. 1998;36:413–7.PubMedGoogle Scholar
  29. 29.
    Pereira M, Vianna G. A proposito de um caso de blastomycose (Pyohemia blastomycotica). Arch Bras Med. 1911;1:63–83.Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Jacqueline B. Ferreira
    • 1
  • Italmar T. Navarro
    • 2
  • Roberta L. Freire
    • 2
  • Gabriela G. Oliveira
    • 1
  • Aline M. Omori
    • 1
  • Donizeti R. Belitardo
    • 1
  • Eiko N. Itano
    • 1
  • Zoilo P. Camargo
    • 3
  • Mario A. Ono
    • 1
  1. 1.Departamento de Ciências Patológicas, Centro de Ciências BiológicasUniversidade Estadual de LondrinaLondrinaBrazil
  2. 2.Departamento de Medicina Veterinária PreventivaUniversidade Estadual de LondrinaLondrinaBrazil
  3. 3.Disciplina de Biologia CelularUniversidade Federal de São PauloSão PauloBrazil

Personalised recommendations