Susceptibilities to Amphotericin B, Fluconazole and Voriconazole of Trichosporon Clinical Isolates
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A total of 35 Trichosporon isolates were collected from the Taiwan Surveillance of Antimicrobial Resistance of Yeasts (TSARY) project from 1999 to 2006, and their identifications as well as drug susceptibilities were determined. The most frequently isolated species was T. asahii (62.9%), and the most common clinical sample that yielded Trichosporon isolates was urine (37.1%). The etiology of all seven invasive trichosporonosis was T. asahii. For the 22 T. asahii isolates, the MIC50 and MIC90 for amphotericin B were 0.25 and 1 μg/mL, respectively. Those for fluconazole were 2 and 4 μg/mL, respectively, and for voriconazole 0.031 and 0.063 μg/mL, respectively. When the intraclass correlation coefficients (ICCs) and agreements were calculated, we found that the MICs of fluconazole obtained from different methods were similar and the inter-method discrepancies were low. Nevertheless, no unanimous MIC of amphotericin B and voriconazole was obtained among different methods.
KeywordsAntifungal susceptibility testing CLSI EUCAST Trichosporon
The authors would like to thank Bristol Myers Squibb for supplying the reference powder of amphotericin B, and Pfizer for fluconazole and voriconazole. They also thank the TSARY hospitals, especially the following 17, for providing clinical Trichosporon isolates: Chang Gung Memorial Hospital at Keelung, Chang Gung Memorial Hospital at Linkou, Chi-Mei Foundation Hospital, Chung Shan Medical Dental College Hospital, Tainan Municipal Hospital, Kuan-Tien General Hospital, Lo-Hsu Foundation Inc. Lo-Tung Poh Ai Hospital, Kaohsiung Military Hospital, Hua-Lien Mennonite Church Hospital, National Cheng-Kung University Hospital, Hua-Lien Hospital, DOH, the Executive Yuan, Show Chwan Memorial Hospital, Tri Service General Hospital, Veterans General Hospital—Taichung, Veterans General Hospital-Kaohsiung, Asia East Memorial Hospital, and Buddhist Tzu-Chi General Hospital. This work was supported in part by the NHRI fellowship research grant to MST and by grants CL-098-PP-04 and ID-099-PP-04 from the NHRI to HJL.
Conflict of interest
The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.
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