, Volume 172, Issue 6, pp 439–446 | Cite as

Variation in Genotype and Higher Virulence of a Strain of Sporothrix schenckii Causing Disseminated Cutaneous Sporotrichosis

  • Zhenying Zhang
  • Xiaoming Liu
  • Xuelian Lv
  • Jingrong Lin


Sporotrichosis is usually a localized, lymphocutaneous disease, but its disseminated type was rarely reported. The main objective of this study was to identify specific DNA sequence variation and virulence of a strain of Sporothrix schenckii isolated from the lesion of disseminated cutaneous sporotrichosis. We confirmed this strain to be S. schenckii by® tubulin and chitin synthase gene sequence analysis in addition to the routine mycological and partial ITS and NTS sequencing. We found a 10-bp deletion in the ribosomal NTS region of this strain, in reference to the sequence of control strains isolated from fixed cutaneous sporotrichosis. After inoculated into immunosuppressed mice, this strain caused more extensive system involvement and showed stronger virulence than the control strain isolated from a fixed cutaneous sporotrichosis. Our study thus suggests that different clinical manifestation of sporotrichosis may be associated with variation in genotype and virulence of the strain, independent of effects due to the immune status of the host.


Sporothrix schenckii Disseminated cutaneous sporotrichosis Genotype Virulence 



This study is funded by National Natural Science Foundation of China.

Conflict of Interest


Supplementary material

11046_2011_9441_MOESM1_ESM.doc (42 kb)
Supplementary material 1 (DOC 42 kb)


  1. 1.
    Miyaji M. Defensive role of granuloma against Sporotrix sckenckii infection. Myopathologia. 1982;80:117–24.Google Scholar
  2. 2.
    Ishizak H, Kawasaki M, Aoki M. Mitochondrial DNA analysis of Sporotrix sckenckii in North and South America. Myopathologia. 1998;142:115–8.CrossRefGoogle Scholar
  3. 3.
    Kong X, Xiao T, Lin J, et al. Relationships among genotypes, virulence and clinical forms of Sporothrix schenckii infection. Clin Microbiol Infect. 2006;12:1077–81.PubMedCrossRefGoogle Scholar
  4. 4.
    Liu X, Lian C, Jin L, et al. Characterization of Sporothrix schenckii by random amplification of polymorphic DNA assay. Chin Med J. 2003;116:239–42.PubMedGoogle Scholar
  5. 5.
    Marimon R, Cano J, Gene J, et al. Sporothrix brasiliensis, S. globosa, and S. Mexicana, three new sporothrix species of clinical interest. J Clin Microbiol. 2007;45:3198–206.PubMedCrossRefGoogle Scholar
  6. 6.
    Liu X, Lin X. A case of disseminated sporotrichosis. J Dermatol. 2001;28:95–9.PubMedGoogle Scholar
  7. 7.
    Graham GC, Mayers P, Henry RJ. A simplified method for the preparation of fungal genomic DNA for PCR and RAPD analysis. Biotechniques. 1994;16:48–50.PubMedGoogle Scholar
  8. 8.
    Liu D, Coloe S, Baird R, et al. Application of PCR to the identification of dermatophyte fungi. J Med Microbiol. 2000;49:493–7.PubMedGoogle Scholar
  9. 9.
    Hajjeh R, McDonnell S, Reef S. Outbreak of sporotrichosis among tree nursery workers. J Invent Dermatal. 1997;176:499–504.CrossRefGoogle Scholar
  10. 10.
    Ishizaki H, Kawasaki M, Aoki M, et al. Mitochondrial DNA analysis of Sporotrix schenckii from China, Korea and Spain. Med Mycol. 2004;45:23–5.CrossRefGoogle Scholar
  11. 11.
    Carlotti A, Chaib F, Couble A, et al. Rapid identification and fingerprinting of Candida krusei by PCR-based amplification of the species-specific repetitive polymorphic sequence CKRS-1. J Clin Microbiol. 1997;35:1337–43.PubMedGoogle Scholar
  12. 12.
    Bain JM, Tavanti A, Davidson AD, et al. Multilocus sequence typing of the pathogenic ungus Aspergillus fumigatus. J Clin Microbiol. 2007;45:1469–77.PubMedCrossRefGoogle Scholar
  13. 13.
    Jackson CJ, Barton RC, Kelly SL, et al. Strains identification of Trichophyton ribrum by specific amplification of subrepeat elements in the ribosomal DNA nontranscribed spacer. J Clin Microbiol. 2000;38:4527–34.PubMedGoogle Scholar
  14. 14.
    Brito MM, Conceicao-Silva F, Morgado FN, et al. Comparison of virulence of different Sporothrix schenckii clinical isolates using experimental murine model. Med Mycol. 2007;45(8):721–9.PubMedCrossRefGoogle Scholar
  15. 15.
    Uenotsuchi T, Takeuchi S, Matsuda T, et al. Differential induction of Th1-prone immunity by human dendritic cells activated with Sporothrix schenckii of cutaneous and visceral origins to determine their different virulence. Int Immunol. 2006;18(12):1637–46.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Zhenying Zhang
    • 1
  • Xiaoming Liu
    • 1
  • Xuelian Lv
    • 2
  • Jingrong Lin
    • 1
  1. 1.Department of DermatologyThe 1st Affiliated Hospital of Dalian Medical UniversityDalianChina
  2. 2.Dalian Hospital of DermatologyDalianChina

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