Adhesive Properties and Hydrolytic Enzymes of Oral Candida albicans Strains
- 263 Downloads
Several virulence factors in Candida albicans strains such as production of hydrolytic enzymes and biofilm formation on surfaces and cells can contribute to their pathogenicity. For this, control of this opportunistic yeast is one of the factors reducing the nosocomial infection. The aim of this study was to investigate biofilm formation on polystyrene and polymethylmethacrylate and the production of hydrolytic enzymes in Candida albicans strains isolated from the oral cavity of patients suffering from denture stomatitis. All strains were identified by macroscopic, microscopic analysis and the ID 32 C system. Our results showed that 50% of the total strains produced phospholipase. Furthermore, protease activity was detected in seven (35%) strains. All Candida albicans strains were beta haemolytic. All C. albicans strains adhered to polystyrene 96-well microtiter plate at different degrees, and the metabolic activity of C. albicans biofilm formed on polymethylmethacrylate did not differ between tested strains. The atomic force micrographs demonstrated that biofilm of Candida albicans strains was organized in small colonies with budding cells.
KeywordsCandida albicans Hydrolytic enzymes Biofilm Polymethylmethacrylate
We gratefully acknowledge Prof. Stefania Zanetti (University of Sassari, Italy) for standard Candida albicans strains and Mr. Moez Abroud from the Centre de Recherche Technologique de Borj Cédria (Tunisia), for technical assistance with the AFM technique.
- 24.Ausubel FM, Brent R, Kingston RE. Current protocols in molecular biology. New York, John Wiley and sons; 1996, 2:13.11.1–13.13.8.Google Scholar
- 26.Aoki S, Ito-Kuwa S, Nakamura Y, Masuhara T. Comparative pathogenicity of wild-type strains and respiratory mutants of Candida albicans in mice. Zol Bakt. 1990;273:332–43.Google Scholar
- 29.Melo ASA, Padovan ACB, Serafim RC, Puzer L, Carmona AK, Juliano Neto L, et al. The Candida albicans AAA ATPase homologue of Saccharomyces cerevisiae Rix7p (YLL034c) is essential for proper morphology, biofilm formation and activity of secreted aspartylproteinases. Genet Mol Res. 2006;5:664–87.PubMedGoogle Scholar
- 38.Kretchmar M, Hube B, Bertsch T, et al. Germ tubes and proteinase activity contribute to virulence of Candida albicans in murine peritonitis. Infect Immun. 1999;67:6637–42.Google Scholar