, Volume 168, Issue 2, pp 51–58 | Cite as

Mice Immunization with Radioattenuated Yeast Cells of Paracoccidiodes brasiliensis: Influence of the Number of Immunizations

  • Estefânia Mara do Nascimento Martins
  • Bernardo Sgarbi Reis
  • Maria Aparecida de Resende
  • Antero Silva Ribeiro de Andrade
  • Alfredo Miranda Goes


Paracoccidioides brasiliensis is the agent of paracoccidioidomycosis, the most prevalent mycosis in Latin America. Up to the moment no vaccine has been reported. The aim of this study was to evaluate the influence of the number of immunizations on the protection elicited by radioattenuated yeast cells of P. brasiliensis. BALB/c mice were divided into two groups that were immunized once (Group 1) or twice (Group 2), respectively. In each group, mice were divided into sub-groups that were challenged 30, 45, or 60 days after the second immunization. Organ colony-forming units (CFUs) was determined 90 days post-challenge. A significant reduction in CFUs recovery was verified in both groups, but it was higher in Group 2. Histologic alterations were observed only in Group 1. The cytokines IL-4, IL-10, and IFN-γ were produced in mice of Group 1. In Group 2, only IFN-γ was significantly detected. IgG2a predominance relative to IgG1 was also observed in Group 2. Altogether, our results indicated that mice immunized once developed a mixed Th1/Th2 response, which was less efficient in the infection control, while a trend to a Th1 pattern was obtained with two immunizations, promoting optimal elimination of P. brasiliensis yeast cells from mice tissues.


Paracoccidioides brasiliensis Vaccine Gamma irradiation Immunization 



This research was supported by Fundação de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG) (CBB580/06), International Atomic Energy Agency (IAEA) (13239/R0), and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).


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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Estefânia Mara do Nascimento Martins
    • 1
    • 2
  • Bernardo Sgarbi Reis
    • 3
  • Maria Aparecida de Resende
    • 2
  • Antero Silva Ribeiro de Andrade
    • 1
  • Alfredo Miranda Goes
    • 3
  1. 1.Laboratório de RadiobiologiaCentro de Desenvolvimento da Tecnologia Nuclear (CDTN)Belo HorizonteBrazil
  2. 2.Departamento de MicrobiologiaUniversidade Federal de Minas Gerais (UFMG)Belo HorizonteBrazil
  3. 3.Departamento de Bioquímica e ImunologiaUniversidade Federal de Minas Gerais (UFMG)Belo HorizonteBrazil

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