Immunohistochemical and immunocytochemical detection of SchS34 antigen in Stachybotrys chartarum spores and spore impacted mouse lungs
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The purpose of this study was to evaluate the distribution of a 34 kD antigen isolated from S. chartarum sensu lato in spores and in the mouse lung 48 h after intra-tracheal instillation of spores by immuno-histochemistry. This antigen was localized in spore walls, primarily in the outer and inner wall layers and on the external wall surfaces with modest labelling observed in cytoplasm. Immuno-histochemistry revealed that in spore impacted mouse lung, antigen was again observed in spore walls, along the outside surface of the outer wall and in the intercellular space surrounding spores. In lung granulomas the labelled antigen formed a diffusate, some 2–3× the size of the long axis of spores, with highest concentrations nearest to spores. Collectively, these observations indicated that this protein not only displayed a high degree of specificity with respect to its location in spores and wall fragments, but also that it slowly diffuses into surrounding lungs.
KeywordsStachybotrys chartarum Antigen Spores Mouse lung Immunohistochemistry Antigen processing
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We thank C. Leggiadro NRC Institute of Marine Biosciences for assistance and excellent technical support. Jiangping Xu prepared the antibodies. This study was supported by a Natural Sciences and Research Council discovery grant administered to TGR and an NSERC IRC to JDM.
- 1.Health Canada. Fungal contamination in public buildings: health effects and investigation methods. Health Canada, Ottawa, ON. 2004. ISBN 0-662-37432-0.Google Scholar
- 2.NAS. Damp indoor air spaces and health. Washington, DC: Institute of Medicine, National Academies Press; 2004.Google Scholar
- 3.WHO. Health and environmental briefing #42 for local health officials. World Health Organization European Centre for Environment and Health, Hermann-Ehlers-Strase, Bonn, Germany, 2004.Google Scholar
- 6.Schram-Bijkerk D; Doekes G, Douwes J, Boeve M, Riedler J, Ueblagger E, von Mutius E, Benz MR, Pershagen G, van Hage M, Scheynius A, Braun-Fahrlander C, Waser M, Brunekreef B. Bacterial and fungal agents in house dust and wheeze in children: the PARSIFAL study. Clin Exp Allergy 2005;35:1272–8.CrossRefGoogle Scholar
- 7.NAS. Clearing the air. Washington, DC: National Academy of Science, National Academy Press; 2000.Google Scholar
- 12.Xu J, Jensen JT, Liang Y, Miller JD. The biology and immogenicity of the 34 kDa antigen of Stachybotrys chartarum sensu lato. Int Biodeterior Biodegrad 2007 (in press).Google Scholar
- 13.Xu J, Liang Y, Miller JD. Characterization of monoclonal antibodies of SchS protein from Stachybotrys chartarum and their application in measurement of S. chartarum antigen by capture ELISA (in press).Google Scholar
- 14.MaCrae KC, Rand TG, Shaw RA, Mason C, Oulton MR, Hastings C, Cherlet T, Thliveris JA, Mantsch HH, MacDonald J, Scott JE. Analysis of pulmonary surfactant by Fourier-transform infrared spectroscopy following exposure to Stachybotrys chartarum (atra) spores. Chem Phys Lipids 2001;110:1–10.CrossRefGoogle Scholar
- 22.Hung LL, Miller JD, Dillon HK, editors. Field guide for the determination of biological contaminants in environmental samples, 2nd ed. American Industrial Hygiene Association, Fairfax, VA; 2005. p. 93–128.Google Scholar
- 23.Nolard N. Moulds and respiratory allergies. Expressions 1997;5:7–9.Google Scholar
- 25.Richard JL, Thurston JR. Effect of aflatoxin on phagocytosis of Aspergillus fumigatus spores by rabbit alveolar macrophages. Appl Micro.1975;30:44–7.Google Scholar
- 26.Sorenson WG. Fungal spores: hazardous to health? Environ Health Perspect 1999; 107 s.3:469–72.Google Scholar
- 29.Bozzola JJ, Russell LD. Electron microscopy. Boston, MA: Jones and Bartlett Publishers; 1992.Google Scholar
- 30.CCAC. Guide to the care and use of experimental animals, vol 1. Canadian Council on Animal Care, Bradda Printing Services Inc., Ottawa, ON; 1993.Google Scholar
- 34.Gregory L, Rand TG, Dearborn D Yike I, Vesper S. Immunocytochemical localization of a hemolysin-like protein in Stachybotrys chartarum spores and spore-impacted mouse and rat lung tissues. Mycopathologia 2002;56:77–85.Google Scholar