, Volume 166, Issue 1, pp 25–33 | Cite as

Fungicidal activity of human monocyte-derived multinucleated giant cells induced in vitro by Paracoccidioides brasiliensis antigen

  • Magda Paula Pereira do Nascimento
  • Angela Maria Victoriano de Campos Soares
  • Luciane Alarcão Dias-Melicio
  • Maria Rita Parise-Fortes
  • Rosana Aparecida Rodrigues Martins
  • Erika Takahashi Nakaira
  • Maria Terezinha Serrão Peraçoli


Multinucleated giant cells (MGC) are characteristic cells in granulomatous disorders such as paracoccidioidomycosis (PCM) and also are formed in vitro from peripheral blood mononuclear cells by several stimuli. In this study, the authors investigated in vitro formation of MGC derived from monocytes of healthy individuals, stimulated with Paracoccidioides brasiliensis antigen (PbAg), compared with other stimuli such as IFN-γ and supernatant of Con-A-stimulated peripheral blood mononuclear cells (CM-ConA). Besides, the fungicidal activity of monocytes and monocyte-derived MGC challenged with P. brasiliensis were compared, at a ratio of one fungus per 50 monocytes. Results demonstrated that PbAg, IFN-γ, and CM-ConA stimuli were able to induce MGC generation, with fusion indices significantly higher than control cultures. Striking results were observed when MGC induced by PbAg and IFN-γ presented higher fungicidal activity than monocytes, submitted to the same stimuli, showing a better capacity of these cells to kill P. brasiliensis. In summary, the results suggest that PbAg is able to induce MGC generation, and these cells presented higher fungicidal activity against P. brasiliensis than monocytes.


Fungicidal activity Multinucleated giant cells Paracoccidioides brasiliensis Interferon-γ 



Brain–heart infusion


Colony forming units


Conditioned medium


Concanavalina A


Foreign body giant cells


Fusion index

GPY medium

Glucose, peptone, yeast extract and agar medium




Langhans giant cells


Multinucleated giant cells




Monoclonal antibody


Paracoccidioides brasiliensis antigens


Paracoccidioides brasiliensis strain 18


Phosphate-buffered saline- ethylenediaminetetraacetic acid

RPMI 1640

Roswell Park Memorial Institute medium


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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Magda Paula Pereira do Nascimento
    • 1
  • Angela Maria Victoriano de Campos Soares
    • 1
  • Luciane Alarcão Dias-Melicio
    • 1
  • Maria Rita Parise-Fortes
    • 2
  • Rosana Aparecida Rodrigues Martins
    • 1
  • Erika Takahashi Nakaira
    • 1
  • Maria Terezinha Serrão Peraçoli
    • 1
  1. 1.Department of Microbiology and ImmunologyBiosciences Institute, São Paulo State University (UNESP)BotucatuBrazil
  2. 2.Department of Dermatology and RadiotherapyMedical School, São Paulo State UniversityBotucatuBrazil

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