Advertisement

Mycopathologia

, Volume 162, Issue 5, pp 347–353 | Cite as

Impact on growth and aflatoxin B1 accumulation by Kluyveromyces isolates at different water activity conditions

  • Mariángeles La Penna
  • Miriam Etcheverry
Article

Abstract

This study showed the impact on germination, mycelial growth and aflatoxin B1 accumulation when interacting Aspergillus aflatoxigenic strains with Kluyveromyces isolates and the effect of water activity on this relationship. Isolates Y14 and Y16 reduced the percentage of germination of all Aspergillus strains and decrease germ tube elongation rate at majority of water activity assayed. Similarly they produced an increase of germination lag phase and lag phase of growth beside decreased growth rate of all Aspergillus strains. At water activities 0.994, 0.982, 0.955 and 0.937, no aflatoxins were produced in paired cultures with isolates Y25, Y22, Y16, and Y14, and Kluyveromyces isolates Y14 and Y16 impact both growth and aflatoxin accumulation at wide range of water activity.

Keywords

biocontrol Kluyveromyces isolates Aspergillus section Flavi aflatoxina B1 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Chulze, S, Bertinetti, C, Dalcero, A, Etcheverry, M, Farnochi, C, Torres, A, Rizzo, I, Varsavsky, E 1989Incidence of aflatoxin, zearalenone and deoxynivalenol on corn in ArgentinaMycotoxin Res5912Google Scholar
  2. 2.
    Torres, A, Ramirez, ML, Reynoso, MM, Rodriguez, M, Chulze, S 1997Natural Co-occurrence of Fusarium species (section Liseola) and Aspergillus flavus Group species, Fumonisin and Aflatoxin in Argentinian CornCereal Res Commun25389392Google Scholar
  3. 3.
    Egmond, HP 1995Mycotoxins: Regulations, quality assurance and reference materialsFood Addit Contam12321330PubMedGoogle Scholar
  4. 4.
    Coallier-Ascah, J, Idziak, E S 1995Interaction between Streptococcus lactis and Aspergillus flavus on production of AflatoxinAppl Environ Microbiol49163167Google Scholar
  5. 5.
    Picco, M, Nesci, A, Barros, G, Cavaglieri, L, Etcheverry, M 1999Aflatoxin B1 and Fumonisin B1 in mixed cultures of Aspergillus flavus and Fusarium proliferatum on maizeNat Toxins7331336CrossRefPubMedGoogle Scholar
  6. 6.
    Björnberg, A, Schnürer, J 1993Inhibition of the growth of grain-storage molds in vitro by the yeast Pichia anomala (Hansen) KurstmanCan J Microbiol39623628CrossRefGoogle Scholar
  7. 7.
    Mc Guire, RG 1994Application of Candida guillermondi in commercial citrus coatings for biocontrol of Penicillium digitatum on grapefruitsBiol Control417CrossRefGoogle Scholar
  8. 8.
    Petersson, S, Jonsson, N, Schnürer, J 1999Pichia anomala as a biocontrol agent during storage of high-moisture feed grain under airtight conditionsPostharvest Biol Technol15175184CrossRefGoogle Scholar
  9. 9.
    Leistner, L 1994Further developments in the utilization of hurdle technology for food preservationJ Food Eng22421432CrossRefGoogle Scholar
  10. 10.
    Leistner, L 1995Principles and application of hurdle technologyGould, GW eds. New Methods of Food PreservationBlackieGlasgow, UK121Google Scholar
  11. 11.
    Penna, M, Nesci, A, Etcheverry, M 2004In vitro studies on the potential for biological control on Aspergillus section Flavi by Kluyveromyces sppLett Appl Microbiol38257264CrossRefPubMedGoogle Scholar
  12. 12.
    Nesci, A, Etcheverry, M 2002Aspergillus section Flavi populations from field maize in ArgentinaLett Appl Microbiol34343348CrossRefPubMedGoogle Scholar
  13. 13.
    Dallyl, H, Fox, A 1980Spoilage of material of reduced water activity by xerophilic fungiGould, GHCorry, EL eds. Society of Applied Bacteriology Technical Series 15Academic PressLondon, UK129139Google Scholar
  14. 14.
    Pitt, J 1979The Genus Penicillium and its Teleomorphic States. Eupenicillium and TalaromycesAcademic PressLondon, UKGoogle Scholar
  15. 15.
    González, HHL, Resnik, SL, Vaamonde, G 1988Influence of inoculum size on growth rate and lag phase of fungi isolated from Argentine cornInt J Food Microbio4111117CrossRefGoogle Scholar
  16. 16.
    Trucksess, MW, Stack, ME, Nesheim, S, Albert, RH, Romer, TR 1994Multifunctional column coupled with liquid chromatography for determination of aflatoxin B1, B2, G1, G2 in corn, almonds, Brazil nuts, peanuts and pistschio nuts: Collaborative studyJ AOAC Int615121521Google Scholar
  17. 17.
    Cuero, R, Smith, JE, Lacey, J 1988Mycotoxin Formation by Aspergillus flavus and Fusarium graminearum in Irradiated Maize Grains in the presence of other FungiJ Food Prot51452456Google Scholar
  18. 18.
    Wicklow, DT, Hesseltine, CW, Shotwell, OL, Adams, GL 1980Interference competition and aflatoxin levels in cornPhytopathology70761764CrossRefGoogle Scholar
  19. 19.
    Marín, S, Sanchis, V, Saenz, R, Ramos, AJ, Viñas, I, Magan, N 1998Ecological determinants for germination and growth of some Aspergillus and Pencillium spp. from maize grainJ Appl Microbiol842536CrossRefPubMedGoogle Scholar
  20. 20.
    Ehrlich, K 1987Effect on aflatoxin production of competition between wild-type and mutant strains of Aspergillus parasiticus Mycopathología979396CrossRefPubMedGoogle Scholar
  21. 21.
    Ehrlich, K, Ciegler, A, Klich, M, Lee, L 1985Fungal competition and mycotoxin production on cornExperientia41691693CrossRefGoogle Scholar
  22. 22.
    El-Gendy, SM, Marth, EH 1981Growth and aflatoxin production by Aspergillus parasiticus in the presence of Lactobacillus casei J Food Prot44211212Google Scholar
  23. 23.
    Etcheverry, M, Magnoli, C, Dalcero, A, Chulze, S, Lecumberry, S 1998Aflatoxin B1, zearalenone and deoxynivalenol production by Aspergillus parasiticus and Fusarium graminearum in interactive cultures on irradiated corn kernelsMycopathología1423742CrossRefPubMedGoogle Scholar
  24. 24.
    Petersson, S, Schnürer, J 1995Biocontrol of mold in high moisture wheat stored in airtight conditions by Pichia anomala, Pichia guillermondi and Saccharomyces cerevisiae Appl Environ Microbiol6110271032PubMedGoogle Scholar
  25. 25.
    Petersson, S, Schnürer, J 1998Pichia anomala as a biocontrol agent of Penicillium roqueforti in high-moisture wheat, rye, barley, and oats stored under airtight conditionsCan J Microbiol44471476CrossRefGoogle Scholar
  26. 26.
    Petersson, S, Hansen, MW, Axberg, K, Hult, K, Schnürer, J 1998Ochratoxin A accumulation in cultures of Penicillium verrucosum with the antagonist yeast Picchia anomala and Saccharomyces cerevisiae Mycol Res10210031008CrossRefGoogle Scholar

Copyright information

© Springer 2006

Authors and Affiliations

  1. 1.Departamento de Microbiología e InmunologíaUniversidad Nacional de Río CuartoRío CuartoArgentina
  2. 2.Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)Buenos AiresArgentina

Personalised recommendations