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Mycopathologia

, Volume 160, Issue 1, pp 9–14 | Cite as

DNA fingerprinting pattern and susceptibility to antifungal drugs in Cryptococcus neoformans variety grubii isolates from Barcelona city and rural environmental samples

  • Yolanda Morera-López
  • Josep Maria Torres-Rodríguez
  • Teresa Jiménez-Cabello
  • Teresa Baró-Tomás
  • Concepción Alía-Aponte
  • Marcia S. Lázera
Article

Abstract

Cryptococcus neoformans var. grubii (serotype A) was isolated from 12 soil samples mixed with pigeon droppings (16.9%) from 71 soil samples in Barcelona and rural areas of Catalonia. C. neoformans was not isolated from indoor dust and Eucalyptus debris. PCR fingerprinting was performed in 22 representative isolates and all of them corresponded to the VNI pattern. Susceptibility testing for the 22 isolates of C. neoformans var. grubii showed that all of them were susceptible to amphotericin B. Three isolates presented MICs (Minimal Inhibitory Concentrations) ≥ 1 μg/ml to Itraconazole, five MICs  ≥ 1 μg/ml to ketoconazole and four were fluconazole resistant, (MICs  ≥ 64 μg/ml), while three of them were shown to have MICs  ≥ 1 μg/ml to voriconazole. In spite that all isolates presented the same DNA fingerprinting pattern, the susceptibility to antifungals is very variable. The possibility of acquiring cryptococcosis infection with primarily resistant environment strains is feasible.

Keywords

Barcelona Cryptococcus neoformans environmental isolates in vitro susceptibility PCR fingerprinting triazoles Barcelona 

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Copyright information

© Springer 2005

Authors and Affiliations

  • Yolanda Morera-López
    • 1
  • Josep Maria Torres-Rodríguez
    • 1
    • 2
  • Teresa Jiménez-Cabello
    • 1
  • Teresa Baró-Tomás
    • 1
    • 2
  • Concepción Alía-Aponte
    • 1
    • 2
  • Marcia S. Lázera
    • 3
  1. 1.Experimental and Clinical Mycology Research Unit (URMEC)Municipal Institute of Medical Research, IMAS, IMIMBarcelonaSpain
  2. 2.Universitat Autònoma de BarcelonaBarcelonaSpain
  3. 3.Mycology ServiceEvandro Chagas Institute of Clinical Research, IPEC/FIOCRUZRio de JaneiroBrasil

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